ID V5E4E7_KALBG Unreviewed; 2383 AA.
AC V5E4E7;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 27-MAR-2024, entry version 60.
DE RecName: Full=Serine/threonine-protein kinase TOR {ECO:0000256|RuleBase:RU364109};
DE EC=2.7.11.1 {ECO:0000256|RuleBase:RU364109};
GN ORFNames=PSEUBRA_SCAF7g04563 {ECO:0000313|EMBL:EST05036.1};
OS Kalmanozyma brasiliensis (strain GHG001) (Yeast) (Pseudozyma brasiliensis).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Kalmanozyma.
OX NCBI_TaxID=1365824 {ECO:0000313|EMBL:EST05036.1, ECO:0000313|Proteomes:UP000019377};
RN [1] {ECO:0000313|Proteomes:UP000019377}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GHG001 {ECO:0000313|Proteomes:UP000019377};
RX PubMed=24356824; DOI=10.1128/genomea.00920-13;
RA Oliveira J.V.D.C., dos Santos R.A.C., Borges T.A., Riano-Pachon D.M.,
RA Goldman G.H.;
RT "Draft genome sequence of Pseudozyma brasiliensis sp. nov. strain GHG001, a
RT high producer of endo-1,4-xylanase isolated from an insect pest of
RT sugarcane.";
RL Genome Announc. 1:E0092013-E0092013(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-seryl-[protein] = ADP + H(+) + O-phospho-L-seryl-
CC [protein]; Xref=Rhea:RHEA:17989, Rhea:RHEA-COMP:9863, Rhea:RHEA-
CC COMP:11604, ChEBI:CHEBI:15378, ChEBI:CHEBI:29999, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:83421, ChEBI:CHEBI:456216; EC=2.7.11.1;
CC Evidence={ECO:0000256|ARBA:ARBA00001433};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-threonyl-[protein] = ADP + H(+) + O-phospho-L-
CC threonyl-[protein]; Xref=Rhea:RHEA:46608, Rhea:RHEA-COMP:11060,
CC Rhea:RHEA-COMP:11605, ChEBI:CHEBI:15378, ChEBI:CHEBI:30013,
CC ChEBI:CHEBI:30616, ChEBI:CHEBI:61977, ChEBI:CHEBI:456216;
CC EC=2.7.11.1; Evidence={ECO:0000256|ARBA:ARBA00000775,
CC ECO:0000256|RuleBase:RU364109};
CC -!- SIMILARITY: Belongs to the PI3/PI4-kinase family.
CC {ECO:0000256|ARBA:ARBA00011031, ECO:0000256|RuleBase:RU364109}.
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DR EMBL; KI545893; EST05036.1; -; Genomic_DNA.
DR RefSeq; XP_016290025.1; XM_016439235.1.
DR STRING; 1365824.V5E4E7; -.
DR GeneID; 27421944; -.
DR eggNOG; KOG0891; Eukaryota.
DR HOGENOM; CLU_000178_7_1_1; -.
DR OMA; MRQHSAK; -.
DR OrthoDB; 8448at2759; -.
DR Proteomes; UP000019377; Unassembled WGS sequence.
DR GO; GO:0032991; C:protein-containing complex; IEA:UniProt.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0106310; F:protein serine kinase activity; IEA:RHEA.
DR GO; GO:0004674; F:protein serine/threonine kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0044877; F:protein-containing complex binding; IEA:InterPro.
DR GO; GO:0035556; P:intracellular signal transduction; IEA:UniProt.
DR GO; GO:0010507; P:negative regulation of autophagy; IEA:UniProt.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR GO; GO:0051128; P:regulation of cellular component organization; IEA:UniProt.
DR GO; GO:0019219; P:regulation of nucleobase-containing compound metabolic process; IEA:UniProt.
DR CDD; cd05169; PIKKc_TOR; 1.
DR Gene3D; 1.20.120.150; FKBP12-rapamycin binding domain; 1.
DR Gene3D; 1.25.10.10; Leucine-rich Repeat Variant; 3.
DR Gene3D; 1.10.1070.11; Phosphatidylinositol 3-/4-kinase, catalytic domain; 1.
DR Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 1.
DR InterPro; IPR011989; ARM-like.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR003152; FATC_dom.
DR InterPro; IPR009076; FRB_dom.
DR InterPro; IPR036738; FRB_sf.
DR InterPro; IPR011009; Kinase-like_dom_sf.
DR InterPro; IPR024585; mTOR_dom.
DR InterPro; IPR000403; PI3/4_kinase_cat_dom.
DR InterPro; IPR036940; PI3/4_kinase_cat_sf.
DR InterPro; IPR018936; PI3/4_kinase_CS.
DR InterPro; IPR003151; PIK-rel_kinase_FAT.
DR InterPro; IPR014009; PIK_FAT.
DR InterPro; IPR026683; TOR_cat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR PANTHER; PTHR11139; ATAXIA TELANGIECTASIA MUTATED ATM -RELATED; 1.
DR PANTHER; PTHR11139:SF9; SERINE_THREONINE-PROTEIN KINASE MTOR; 1.
DR Pfam; PF11865; DUF3385; 1.
DR Pfam; PF02259; FAT; 1.
DR Pfam; PF02260; FATC; 1.
DR Pfam; PF08771; FRB_dom; 1.
DR Pfam; PF00454; PI3_PI4_kinase; 1.
DR SMART; SM01346; DUF3385; 1.
DR SMART; SM01343; FATC; 1.
DR SMART; SM00146; PI3Kc; 1.
DR SMART; SM01345; Rapamycin_bind; 1.
DR SUPFAM; SSF48371; ARM repeat; 2.
DR SUPFAM; SSF47212; FKBP12-rapamycin-binding domain of FKBP-rapamycin-associated protein (FRAP); 1.
DR SUPFAM; SSF56112; Protein kinase-like (PK-like); 1.
DR PROSITE; PS51189; FAT; 1.
DR PROSITE; PS51190; FATC; 1.
DR PROSITE; PS00915; PI3_4_KINASE_1; 1.
DR PROSITE; PS00916; PI3_4_KINASE_2; 1.
DR PROSITE; PS50290; PI3_4_KINASE_3; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|RuleBase:RU364109};
KW Kinase {ECO:0000256|ARBA:ARBA00022777, ECO:0000256|RuleBase:RU364109};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741,
KW ECO:0000256|RuleBase:RU364109};
KW Reference proteome {ECO:0000313|Proteomes:UP000019377};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Serine/threonine-protein kinase {ECO:0000256|ARBA:ARBA00022527,
KW ECO:0000256|RuleBase:RU364109};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU364109}.
FT DOMAIN 1253..1808
FT /note="FAT"
FT /evidence="ECO:0000259|PROSITE:PS51189"
FT DOMAIN 1984..2297
FT /note="PI3K/PI4K catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50290"
FT DOMAIN 2351..2383
FT /note="FATC"
FT /evidence="ECO:0000259|PROSITE:PS51190"
FT REGION 2266..2322
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2272..2304
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2383 AA; 268535 MW; 1FD4EF4D903133F9 CRC64;
MSVVGVHQND ALNRIFAGLR SRDEASRQAA GEELKSHVAF VVSELKGDSL STFNNDLHRR
IFELTHSQHV HEKLGGIIAI EDLIEQESED NSARLYRFYQ YLKPNLPCND ASVMIAASRA
LGRVAYHGGQ SLGEQFIEYE VLRALDFLQA GDRNESGRYA AVLIIREMAK KVPQQFHPYV
PRVLDRIWVA LRDVRVIVRE GAAEAMGACL GIIAAREKQM GSYFFESIYE EAEKGLKMSA
AEAIHGSLLA VQQLLQHSKT FMRNRFQRAC ELVFRLHKHR DPLIRRTITN LVPVLARYDP
HYFAEEHLGA VMSILTEQLR KEKDRSPKES AQTFETIGFV AAAMGPKMKP FIEPVLACVK
EGLQMRGKKN APPEGPIFLC IGNLATAVGP HLTRYMHDLL DLMFSCGLSI PLVTALDGIV
KAIPPLMKVV QDRLLDMLSM TLIGQPYRPL GAPASLRPSA NASRDVGAAQ TVESKGVETI
TVALQTLGRF DFQGHILNEF VRNCTLPYLE DDHAAVRQAA AETCADLFVN DPICRQTSMH
AIEVVNDVLD KLMTVGIADP DPELRWTVLS KFGAQEQFDR HLAQSEYVRS LFIALNDEKF
KVREVAIVII GRLAKHNPAY VMPSLRKALI QLLTELEYST VSRHKEEAAK LLTEVVRASQ
RLVKSYALPM LEVLLPKAND PSVGVAARVM ECLGELAKVG GEDLAPNVDQ LMRLAIDQLS
STAPGSSTAK RDAALKTLGL VASNTGHVVN PYLTYRNLLG TVVKILKTEQ SKPVRRETIR
VMGILGALDP YRYKLLEKNG DEGQDETSKG SGTDLFELAL AIGTSTDDYY QNIAIDALIT
ILKDPSLSTH HHAVIEAIMY MFKTQGLKCV TFLPQIIPAF LNVIRTCGTG LSEFYFQQLA
ILISIIKQHV RSHLEPIFEL VQENWNPNSS IQLTIVSLVE AVAKALEGEF KSYLPILLPN
MLQTLDGEIT SKRQPTLLRI LQAFYVFGSN IEEYLHLVLP VVVKMFERPD ASQALRRSAI
ITIGNLSRKV SFCDHASRVI HPLVRVLPTG STEIRNAVME TLSALVVQLG PSYAIFIPVV
NKVLVQNRIQ HPGYNQLVTK LLNGERLPQD LSANDNALGS KVDESPQAEA NKMTVNQQHL
KQAWDTSKVS TSEDWQEWLR RMAVEFMRES PSHALRACRS LADVYPALAY GLFNVAFVSC
WTELYEQYQS DLVKALETAF DAPEVPGDVV HMLLNLAEFM EHDDKALPIN IRVLGDRAYK
FHSYAKALHY KEAEFLTDPS PQVVESLIDI NTKLQQSDAA FGALTYAREH LDITHHEEWY
EKLHRWEEAL AAYDRKAMLD PDDYPVAFGK MRCLHALGEW EHLSDLVQQK WGRADMEDRR
HMAPLAAAAA WSLGQWDTMD DYISAMRSDS SERSFYRAIL HTHRSQRAAA NKQIAKARES
LDSELTALIS ESYGRAYDLM VRTQMLSELE EALAYKLDYK EQPDRQATIR STWMKRLKGC
QPEVEVWQRI LSVRSIVLTP ADDTETWIKF ANLCRKSGRM VLAEKTLNSL LGPERTNADP
RSPIGPRAPP PVIYAHLKFM WASGARIESL SYLQEFTMNL AEDLGVHTVD EHGNLVTQDW
QSSPRLGEFA RLLARCFFKQ GEWQMSLREN WVLDDNSNVI ESYRRATELD RNWYKAWHAW
ALANFEIISH HEERNDQITP QMIAASIVPS VQGFFRSIAL ASGNSLQDTL RLLTLWFKYG
HQEDVSQAVS EGFASVIVDT WLEVIPQIIA RITAPSPRVR RLIHNLLSDV GLAHPQALVY
PLTVAAKSPS HMRIQAAMGI MDNVREHSPV LVEQALLVSN ELIRVAILWH EMWHEGLEEA
SRLYFTEHNI DAMFATLEPL HDALEKGPET LRETSFAQTH GRDLAEAREC GRRFRQYGDI
SDLNQAWDLY YHVFKKITKQ LPAGNSVQLD LQYVSPKLLA MRDLELAVPG TYQSGKPIVC
ITRFEQIVLV IASKQHPRRL KMKGSDGKTY QYLLKGHEDL RQDERVMQLF GLVNTLLSID
SESYKRRLEI RRFPVIPLSP NTGMIGWVEH TDTLHVLIKE YREQHKILLN IEHRLMLQMA
PDYDHLTLMQ KVEVFEYALD NTPGQDLYRV LWLKSRNSES WLERRLAYTR SLAVSSVAGY
ILGLGDRHPS NLLLDRLTGQ IVHIDFGDCF EIACHRPKFP EKVPFRLTRM LVNAMEVGGI
KGTFKVTAEN TMRVLRDNKE SVLALLEAFV HDPLISWRLV ADEAAPDAQE NEAAPTETTT
TTTTAVNTTA NVGASGTVPA NQRESDTEEP AKGGAIAAPT GVPHDMRNQR ALEVVRRIQN
KLAGRDYNPS ESLSVAAQIE RLVQDATSKE NLCCAFVGWC SFW
//
