ID V5EBU6_KALBG Unreviewed; 658 AA.
AC V5EBU6;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:EST07906.1};
GN ORFNames=PSEUBRA_SCAF2g03003 {ECO:0000313|EMBL:EST07906.1};
OS Kalmanozyma brasiliensis (strain GHG001) (Yeast) (Pseudozyma brasiliensis).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Kalmanozyma.
OX NCBI_TaxID=1365824 {ECO:0000313|EMBL:EST07906.1, ECO:0000313|Proteomes:UP000019377};
RN [1] {ECO:0000313|Proteomes:UP000019377}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GHG001 {ECO:0000313|Proteomes:UP000019377};
RX PubMed=24356824; DOI=10.1128/genomea.00920-13;
RA Oliveira J.V.D.C., dos Santos R.A.C., Borges T.A., Riano-Pachon D.M.,
RA Goldman G.H.;
RT "Draft genome sequence of Pseudozyma brasiliensis sp. nov. strain GHG001, a
RT high producer of endo-1,4-xylanase isolated from an insect pest of
RT sugarcane.";
RL Genome Announc. 1:E0092013-E0092013(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycerol + an acyl-CoA = a triacyl-sn-glycerol
CC + CoA; Xref=Rhea:RHEA:10868, ChEBI:CHEBI:17815, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:58342, ChEBI:CHEBI:64615; EC=2.3.1.20;
CC Evidence={ECO:0000256|ARBA:ARBA00001039};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a fatty acyl-CoA + a long chain fatty alcohol = a wax ester +
CC CoA; Xref=Rhea:RHEA:38443, ChEBI:CHEBI:10036, ChEBI:CHEBI:17135,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:77636; EC=2.3.1.75;
CC Evidence={ECO:0000256|ARBA:ARBA00029340};
CC -!- PATHWAY: Glycerolipid metabolism; triacylglycerol biosynthesis.
CC {ECO:0000256|ARBA:ARBA00004771}.
CC -!- PATHWAY: Lipid metabolism. {ECO:0000256|ARBA:ARBA00005189}.
CC -!- SIMILARITY: Belongs to the long-chain O-acyltransferase family.
CC {ECO:0000256|ARBA:ARBA00009587}.
CC -!- SIMILARITY: In the N-terminal section; belongs to the long-chain O-
CC acyltransferase family. {ECO:0000256|ARBA:ARBA00024360}.
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DR EMBL; KI545862; EST07906.1; -; Genomic_DNA.
DR RefSeq; XP_016292895.1; XM_016437400.1.
DR AlphaFoldDB; V5EBU6; -.
DR STRING; 1365824.V5EBU6; -.
DR GeneID; 27420072; -.
DR eggNOG; ENOG502S5A5; Eukaryota.
DR HOGENOM; CLU_361371_0_0_1; -.
DR OMA; LMIKIHH; -.
DR OrthoDB; 1700647at2759; -.
DR UniPathway; UPA00282; -.
DR Proteomes; UP000019377; Unassembled WGS sequence.
DR GO; GO:0004144; F:diacylglycerol O-acyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR GO; GO:0019432; P:triglyceride biosynthetic process; IEA:UniProtKB-UniPathway.
DR InterPro; IPR045034; O-acyltransferase_WSD1-like.
DR InterPro; IPR009721; O-acyltransferase_WSD1_C.
DR InterPro; IPR004255; O-acyltransferase_WSD1_N.
DR PANTHER; PTHR31650:SF1; DIACYGLYCEROL O-ACYLTRANSFERASE TGS4-RELATED; 1.
DR PANTHER; PTHR31650; O-ACYLTRANSFERASE (WSD1-LIKE) FAMILY PROTEIN; 1.
DR Pfam; PF06974; WS_DGAT_C; 1.
DR Pfam; PF03007; WS_DGAT_cat; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315};
KW Glycerol metabolism {ECO:0000256|ARBA:ARBA00022798};
KW Lipid biosynthesis {ECO:0000256|ARBA:ARBA00022516};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Reference proteome {ECO:0000313|Proteomes:UP000019377};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 251..360
FT /note="O-acyltransferase WSD1-like N-terminal"
FT /evidence="ECO:0000259|Pfam:PF03007"
FT DOMAIN 498..632
FT /note="O-acyltransferase WSD1 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF06974"
FT REGION 1..126
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 215..235
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 44..68
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 80..96
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 658 AA; 74081 MW; 9ECEE027AA8D0D14 CRC64;
MTDGKPSHGR GTAAEQGAAL RAAADRETGQ THAHEHRSSR RSSRRVSAHE SPRTSERVIE
EDDARHHRKS SRRTSRRHAD HRHSKEDHRE REPSKRVGTA AAQGAAFRKG EEPAPPSAEQ
TREARERHIR AAYGETPKLG KGSPNKPPVY PMTGIDNLAL LMEDDSYTTS CFSIYMFKTE
LDLETVDNFF EVLAETYPKY RYVVDLDPKL SKKLEKQTLK GDPGSSPPRG DFDEGRRTRY
AKGLKAGSLF SPARWRFVPD FDLKENIEHV AQCPDGGDDK ALNKLAGHFI GRHYDYSKPI
WEAMVVNGLN TSDGGRSALM IKIHHCFSDG QGMIQSYHAA LGALEQGVGI RDIQNKVDTN
TSQNKRPGQR EDKPTVLGTI QHAGHLARGL YFRKRKSFLY QEKRKRAAGR LYCHSEGIDM
DDIKMIRKAY GTEKFNLTLN DVALAILARA LRIASERVDP SRRHKDKRVA IFVPISVRPK
GDWSLSNATT GSIAWLRFEK EAKPDFEEQL AQVHREMGRV KRSYGPKILY KVFDTFSKRR
ITYLPNYPLL RSIYYRAFRE YHVATNVPGP PKPVKFGDHE AYSYHVLPPS SPGKSTMAIG
MISYANDFSL AVSCDDVPEF KHLAEEICKA FQDAAAQIIE AAHEKLGSKK EGGATKAQ
//
