ID V5EYQ7_KALBG Unreviewed; 826 AA.
AC V5EYQ7;
DT 22-JAN-2014, integrated into UniProtKB/TrEMBL.
DT 22-JAN-2014, sequence version 1.
DT 27-MAR-2024, entry version 52.
DE RecName: Full=Palmitoyltransferase {ECO:0000256|RuleBase:RU079119};
DE EC=2.3.1.225 {ECO:0000256|RuleBase:RU079119};
GN ORFNames=PSEUBRA_SCAF13g01865 {ECO:0000313|EMBL:EST08943.1};
OS Kalmanozyma brasiliensis (strain GHG001) (Yeast) (Pseudozyma brasiliensis).
OC Eukaryota; Fungi; Dikarya; Basidiomycota; Ustilaginomycotina;
OC Ustilaginomycetes; Ustilaginales; Ustilaginaceae; Kalmanozyma.
OX NCBI_TaxID=1365824 {ECO:0000313|EMBL:EST08943.1, ECO:0000313|Proteomes:UP000019377};
RN [1] {ECO:0000313|Proteomes:UP000019377}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GHG001 {ECO:0000313|Proteomes:UP000019377};
RX PubMed=24356824; DOI=10.1128/genomea.00920-13;
RA Oliveira J.V.D.C., dos Santos R.A.C., Borges T.A., Riano-Pachon D.M.,
RA Goldman G.H.;
RT "Draft genome sequence of Pseudozyma brasiliensis sp. nov. strain GHG001, a
RT high producer of endo-1,4-xylanase isolated from an insect pest of
RT sugarcane.";
RL Genome Announc. 1:E0092013-E0092013(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=hexadecanoyl-CoA + L-cysteinyl-[protein] = CoA + S-
CC hexadecanoyl-L-cysteinyl-[protein]; Xref=Rhea:RHEA:36683, Rhea:RHEA-
CC COMP:10131, Rhea:RHEA-COMP:11032, ChEBI:CHEBI:29950,
CC ChEBI:CHEBI:57287, ChEBI:CHEBI:57379, ChEBI:CHEBI:74151;
CC EC=2.3.1.225; Evidence={ECO:0000256|ARBA:ARBA00001870,
CC ECO:0000256|RuleBase:RU079119};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- DOMAIN: The DHHC domain is required for palmitoyltransferase activity.
CC {ECO:0000256|RuleBase:RU079119}.
CC -!- SIMILARITY: Belongs to the DHHC palmitoyltransferase family.
CC AKR/ZDHHC17 subfamily. {ECO:0000256|ARBA:ARBA00010104}.
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DR EMBL; KI545855; EST08943.1; -; Genomic_DNA.
DR RefSeq; XP_016293932.1; XM_016434521.1.
DR AlphaFoldDB; V5EYQ7; -.
DR STRING; 1365824.V5EYQ7; -.
DR GeneID; 27417128; -.
DR eggNOG; KOG0509; Eukaryota.
DR HOGENOM; CLU_012510_0_0_1; -.
DR OMA; FWVGFRY; -.
DR OrthoDB; 33889at2759; -.
DR Proteomes; UP000019377; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0019706; F:protein-cysteine S-palmitoyltransferase activity; IEA:UniProtKB-EC.
DR Gene3D; 1.25.40.20; Ankyrin repeat-containing domain; 2.
DR InterPro; IPR002110; Ankyrin_rpt.
DR InterPro; IPR036770; Ankyrin_rpt-contain_sf.
DR InterPro; IPR001594; Palmitoyltrfase_DHHC.
DR PANTHER; PTHR24161; ANK_REP_REGION DOMAIN-CONTAINING PROTEIN-RELATED; 1.
DR PANTHER; PTHR24161:SF17; PALMITOYLTRANSFERASE HIP14; 1.
DR Pfam; PF12796; Ank_2; 2.
DR Pfam; PF01529; DHHC; 1.
DR SMART; SM00248; ANK; 5.
DR SUPFAM; SSF48403; Ankyrin repeat; 1.
DR PROSITE; PS50297; ANK_REP_REGION; 2.
DR PROSITE; PS50088; ANK_REPEAT; 4.
DR PROSITE; PS50216; DHHC; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000256|ARBA:ARBA00023315,
KW ECO:0000256|RuleBase:RU079119};
KW ANK repeat {ECO:0000256|ARBA:ARBA00023043, ECO:0000256|PROSITE-
KW ProRule:PRU00023}; Lipoprotein {ECO:0000256|ARBA:ARBA00023288};
KW Membrane {ECO:0000256|RuleBase:RU079119};
KW Palmitate {ECO:0000256|ARBA:ARBA00023139};
KW Reference proteome {ECO:0000313|Proteomes:UP000019377};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|RuleBase:RU079119};
KW Transmembrane {ECO:0000256|RuleBase:RU079119};
KW Transmembrane helix {ECO:0000256|RuleBase:RU079119}.
FT TRANSMEM 323..345
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU079119"
FT TRANSMEM 380..398
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU079119"
FT TRANSMEM 410..427
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU079119"
FT TRANSMEM 508..532
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU079119"
FT TRANSMEM 563..584
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU079119"
FT REPEAT 105..137
FT /note="ANK"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00023"
FT REPEAT 142..171
FT /note="ANK"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00023"
FT REPEAT 210..242
FT /note="ANK"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00023"
FT REPEAT 243..275
FT /note="ANK"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00023"
FT DOMAIN 461..598
FT /note="Palmitoyltransferase DHHC"
FT /evidence="ECO:0000259|Pfam:PF01529"
FT REGION 1..71
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 782..801
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 826 AA; 88901 MW; F52DBAA422CD0642 CRC64;
MAAPGTPLNS SATQAVATSS ANGLTDPLHS SSRSPQPTQQ NGSSVLDTLP SGSNGLGTRA
PSRAHTPGQD QMPLTIHTAA QRGDLPAIMR LVDSGRATVH DRDEDSITPL HWAAINAQLA
TCRYLLDHGA EVDALGGDLV ASPLQWAARN GHVYVQELLC SRGADPTITD SQGFNALHLT
VHSSAVMPLV FMLQQPSLSS PEGLDSTDSQ GHTALMWAAY QGDAISVDIL LKHGSDVHKR
DGAGLTAMHW AVVKGNRLCI RLLADAKADL LAKEDSGKTP RDMAMELKSI GAYRKAMADI
GLEEDGRRKQ RTFGASTDTK ARLAIMVVPF AALGFIFATF ASLPWYTATP FAAAEFFGMH
HIVTRVILDP QEHDFLQRSN YFLAIVSGSI VWVGWEWLHK LASGTPGYAA NNLVFALSIL
VCSWKLFRAA SISPGYAPLA PSALHRREVV TQLAQQGRLN GQTYCVACMA RKPMRSKHCK
LCKRCVARHD HHCPWVANCI GIDNHRQFLL FVGALVMGIL QFIYLSVVYY SVNAPPYTPL
PDSTYETCHL PAFLCTATTY DPFLLGVTLW AALQLTWTVI LLVAQGWQIA RQMTTLEVSN
LGRFGFMGGK GGQSYAGQTN FIAQHSAQAR AQPGSTVEGA TDRLQGIQKA QLADNGEEGS
GSSSRPHAHS KVDFVKRVCS SGGGWLLSIV GLDLYTRGKA GEGLKRASAA ANPFDHGPLA
NCKDFWSKGQ DLNVDYATLY DLPAEISPGH CEVMPFLVDT VTGRGWGGYA RAGNVYSLLR
SDAEEGDEEE EEGREQGMGR RWSMWGSVKG AAGGGLLPTT NAGHAA
//
