ID V6KS34_STRRC Unreviewed; 531 AA.
AC V6KS34;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE RecName: Full=Tryptophan halogenase {ECO:0008006|Google:ProtNLM};
GN ORFNames=M878_11230 {ECO:0000313|EMBL:EST34206.1};
OS Streptomyces roseochromogenus subsp. oscitans DS 12.976.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1352936 {ECO:0000313|EMBL:EST34206.1, ECO:0000313|Proteomes:UP000017984};
RN [1] {ECO:0000313|EMBL:EST34206.1, ECO:0000313|Proteomes:UP000017984}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DS 12.976 {ECO:0000313|EMBL:EST34206.1,
RC ECO:0000313|Proteomes:UP000017984};
RX PubMed=24407645;
RA Ruckert C., Kalinowski J., Heide L., Apel A.K.;
RT "Draft Genome Sequence of Streptomyces roseochromogenes subsp. oscitans DS
RT 12.976, Producer of the Aminocoumarin Antibiotic Clorobiocin.";
RL Genome Announc. 2:e01147-e01113(2014).
CC -!- SIMILARITY: Belongs to the flavin-dependent halogenase family.
CC Bacterial tryptophan halogenase subfamily.
CC {ECO:0000256|ARBA:ARBA00038396}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EST34206.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AWQX01000085; EST34206.1; -; Genomic_DNA.
DR RefSeq; WP_023546221.1; NZ_CM002285.1.
DR AlphaFoldDB; V6KS34; -.
DR SMR; V6KS34; -.
DR STRING; 1352936.M878_11230; -.
DR PATRIC; fig|1352936.5.peg.2384; -.
DR HOGENOM; CLU_022247_0_0_11; -.
DR OrthoDB; 8868802at2; -.
DR Proteomes; UP000017984; Chromosome.
DR GO; GO:0004497; F:monooxygenase activity; IEA:InterPro.
DR GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR Gene3D; 3.50.50.60; FAD/NAD(P)-binding domain; 1.
DR InterPro; IPR036188; FAD/NAD-bd_sf.
DR InterPro; IPR006905; Flavin_halogenase.
DR InterPro; IPR033856; Trp_halogen.
DR PANTHER; PTHR43747; FAD-BINDING PROTEIN; 1.
DR PANTHER; PTHR43747:SF4; FLAVIN-DEPENDENT TRYPTOPHAN HALOGENASE; 1.
DR Pfam; PF04820; Trp_halogenase; 1.
DR PIRSF; PIRSF011396; Trp_halogenase; 1.
DR SUPFAM; SSF51905; FAD/NAD(P)-binding domain; 1.
PE 3: Inferred from homology;
KW FAD {ECO:0000256|PIRSR:PIRSR011396-2};
KW Flavoprotein {ECO:0000256|PIRSR:PIRSR011396-2};
KW Nucleotide-binding {ECO:0000256|PIRSR:PIRSR011396-2};
KW Reference proteome {ECO:0000313|Proteomes:UP000017984}.
FT ACT_SITE 80
FT /evidence="ECO:0000256|PIRSR:PIRSR011396-1"
FT BINDING 80
FT /ligand="7-chloro-L-tryptophan"
FT /ligand_id="ChEBI:CHEBI:58713"
FT /evidence="ECO:0000256|PIRSR:PIRSR011396-2"
FT BINDING 349
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR011396-2"
FT BINDING 358
FT /ligand="L-tryptophan"
FT /ligand_id="ChEBI:CHEBI:57912"
FT /evidence="ECO:0000256|PIRSR:PIRSR011396-2"
FT BINDING 362
FT /ligand="FAD"
FT /ligand_id="ChEBI:CHEBI:57692"
FT /evidence="ECO:0000256|PIRSR:PIRSR011396-2"
SQ SEQUENCE 531 AA; 60280 MW; A3D135557B7793BE CRC64;
MSDNRIRKIL VLGGGTTGWM SAAYLAKALG SSAEVTVLEA PSVPRIGVGE ATIPNLQKVF
FDFLGLSEEE WMPECNASFK LGIRYINWRT PGSGEALGRE HQGGKDHFDH VFGVLPSHEN
LPLSHYWTHK KLTGQTDEPF DQACYTQPQI FDRNLSPRYL DGTRWASYAW HFDAQKVADF
LGRFSVEKLG VQHIQDKFVG ADVDQRGHIV AVKTEEGRRL EADLFIDCSG FRSLLMNGVM
KEPFLDMSDH LLNDRAVATQ IPHDDENNGV EPFTSAIAMS AGWTWKIPML GRFGTGYVYS
SRFTSQDEAT QEFCRMWGID PETHPLNHVK FRVGRNRRAW VKNCIGLGLA NQFVEPLEST
GIYFVYAALY QLVKHFPDKS FDPVLVNRFN HEVEEMFDDT RDFIQGHFSF APRNDTPYWR
ACKELELAPR FVEKVKEYKS GLGVNLPVTD EAGYYGNFEA EFRNFWSNAN YYCILAGLGE
LPDNPMPLLA HQPESIASAE AVFEEIKKRR EELLATLPST HEYLLKLHGK A
//
