ID V6KTN7_STRRC Unreviewed; 703 AA.
AC V6KTN7;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 51.
DE RecName: Full=DNA topoisomerase (ATP-hydrolyzing) {ECO:0000256|ARBA:ARBA00012895};
DE EC=5.6.2.2 {ECO:0000256|ARBA:ARBA00012895};
GN ORFNames=M878_05430 {ECO:0000313|EMBL:EST35502.1};
OS Streptomyces roseochromogenus subsp. oscitans DS 12.976.
OC Bacteria; Actinomycetota; Actinomycetes; Kitasatosporales;
OC Streptomycetaceae; Streptomyces.
OX NCBI_TaxID=1352936 {ECO:0000313|EMBL:EST35502.1, ECO:0000313|Proteomes:UP000017984};
RN [1] {ECO:0000313|EMBL:EST35502.1, ECO:0000313|Proteomes:UP000017984}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DS 12.976 {ECO:0000313|EMBL:EST35502.1,
RC ECO:0000313|Proteomes:UP000017984};
RX PubMed=24407645;
RA Ruckert C., Kalinowski J., Heide L., Apel A.K.;
RT "Draft Genome Sequence of Streptomyces roseochromogenes subsp. oscitans DS
RT 12.976, Producer of the Aminocoumarin Antibiotic Clorobiocin.";
RL Genome Announc. 2:e01147-e01113(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP-dependent breakage, passage and rejoining of double-
CC stranded DNA.; EC=5.6.2.2; Evidence={ECO:0000256|ARBA:ARBA00000185};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|ARBA:ARBA00001946};
CC -!- SIMILARITY: Belongs to the type II topoisomerase GyrB family.
CC {ECO:0000256|ARBA:ARBA00010708}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:EST35502.1}.
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DR EMBL; AWQX01000050; EST35502.1; -; Genomic_DNA.
DR RefSeq; WP_023545088.1; NZ_CM002285.1.
DR AlphaFoldDB; V6KTN7; -.
DR STRING; 1352936.M878_05430; -.
DR PATRIC; fig|1352936.5.peg.1165; -.
DR HOGENOM; CLU_006146_4_1_11; -.
DR OrthoDB; 9802808at2; -.
DR Proteomes; UP000017984; Chromosome.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0034335; F:DNA negative supercoiling activity; IEA:UniProt.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006265; P:DNA topological change; IEA:InterPro.
DR CDD; cd16928; HATPase_GyrB-like; 1.
DR CDD; cd00822; TopoII_Trans_DNA_gyrase; 1.
DR Gene3D; 3.30.230.10; -; 1.
DR Gene3D; 3.40.50.670; -; 1.
DR Gene3D; 3.30.565.10; Histidine kinase-like ATPase, C-terminal domain; 1.
DR InterPro; IPR002288; DNA_gyrase_B_C.
DR InterPro; IPR003594; HATPase_C.
DR InterPro; IPR036890; HATPase_C_sf.
DR InterPro; IPR020568; Ribosomal_Su5_D2-typ_SF.
DR InterPro; IPR014721; Ribsml_uS5_D2-typ_fold_subgr.
DR InterPro; IPR001241; Topo_IIA.
DR InterPro; IPR013760; Topo_IIA-like_dom_sf.
DR InterPro; IPR000565; Topo_IIA_B.
DR InterPro; IPR013759; Topo_IIA_B_C.
DR InterPro; IPR013506; Topo_IIA_bsu_dom2.
DR InterPro; IPR018522; TopoIIA_CS.
DR InterPro; IPR006171; TOPRIM_domain.
DR PANTHER; PTHR45866:SF1; DNA GYRASE SUBUNIT B, MITOCHONDRIAL; 1.
DR PANTHER; PTHR45866; DNA GYRASE/TOPOISOMERASE SUBUNIT B; 1.
DR Pfam; PF00204; DNA_gyraseB; 1.
DR Pfam; PF00986; DNA_gyraseB_C; 1.
DR Pfam; PF02518; HATPase_c; 1.
DR Pfam; PF01751; Toprim; 1.
DR PRINTS; PR01159; DNAGYRASEB.
DR PRINTS; PR00418; TPI2FAMILY.
DR SMART; SM00387; HATPase_c; 1.
DR SMART; SM00433; TOP2c; 1.
DR SUPFAM; SSF55874; ATPase domain of HSP90 chaperone/DNA topoisomerase II/histidine kinase; 1.
DR SUPFAM; SSF54211; Ribosomal protein S5 domain 2-like; 1.
DR SUPFAM; SSF56719; Type II DNA topoisomerase; 1.
DR PROSITE; PS00177; TOPOISOMERASE_II; 1.
DR PROSITE; PS50880; TOPRIM; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW DNA-binding {ECO:0000256|ARBA:ARBA00023125};
KW Isomerase {ECO:0000256|ARBA:ARBA00023235, ECO:0000313|EMBL:EST35502.1};
KW Magnesium {ECO:0000256|ARBA:ARBA00022842};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000017984};
KW Topoisomerase {ECO:0000256|ARBA:ARBA00023029}.
FT DOMAIN 476..590
FT /note="Toprim"
FT /evidence="ECO:0000259|PROSITE:PS50880"
SQ SEQUENCE 703 AA; 76779 MW; ABC4BDA337FC2EB6 CRC64;
MSTLPAPAAG SRPQDGSDYT ARHLMVLEGL EAVRKRPGMY IGSSDSRGLM HCLWEIIDNA
VDEALAGACD YIEVILHDDG SVEVTDNGRG IPVDTEPRTG LSGVEIAYTK LHAGGKFGGG
SYAASGGLHG VGASVVNALS ARLDVEVDRH GRTHAISFRR GTPGTYTGPG PDAPFTPAQG
LRQTTKIPKS RTGTRVRYWA DRQSFLKDAK LSLENLHQRA RQTAFLVPGL TIVVRDEFGL
GEGGSKGEES FRFDGGISEF CEFLASDTPV CDILRFSGQG TFKETVPVLD EHGQMTPTEV
TRELGVDVAL RWGTGYDTTV RSFVNIIATP KGGTHVAGFE QAVGKTLNEV LRAKKLLRVA
EDDIAKDDAL EGLTAVVTVR LAEPQFEGQT KEVLGTSAAR RIVNNVISKE LKAFLTSTKR
DAAQQARVVM EKAVAAARTR IAARQHKDAQ RRKSALESSS LPAKLADCRS DDVDRSELFI
VEGDSALGTA KLARNSEFQA LLPIRGKILN VQRSSVSDML KNAECGAIIQ VIGAGSGRTF
DIDTARYGKI IMMTDADVDG SHIRTLLLTL FHRYMRPMVE AGRVFAAVPP LHRIELVQPR
KGQERYVYTY SDRELRDKLM EFQSKGIRYK DSVQRYKGLG EMDADQLAET TMDPRHRTLR
RINLTDLEAA EGVFDLLMGN DVAPRKEFIT SSAATLDRSR IDV
//
