ID V6U372_GIAIN Unreviewed; 616 AA.
AC V6U372;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 23.
DE RecName: Full=FAD synthase {ECO:0000256|ARBA:ARBA00012393};
DE EC=2.7.7.2 {ECO:0000256|ARBA:ARBA00012393};
DE AltName: Full=FAD pyrophosphorylase {ECO:0000256|ARBA:ARBA00031145};
DE AltName: Full=FMN adenylyltransferase {ECO:0000256|ARBA:ARBA00031871};
GN ORFNames=GSB_150799 {ECO:0000313|EMBL:ESU45082.1};
OS Giardia intestinalis (Giardia lamblia).
OC Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX NCBI_TaxID=5741 {ECO:0000313|EMBL:ESU45082.1, ECO:0000313|Proteomes:UP000018040};
RN [1] {ECO:0000313|Proteomes:UP000018040}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=GS {ECO:0000313|Proteomes:UP000018040};
RA Adam R., Dahlstrom E., Martens C., Bruno D., Barbian K., Porcella S.F.,
RA Nash T.;
RT "Genome sequencing of Giardia lamblia Genotypes A2 and B isolates (DH and
RT GS) and comparative analysis with the genomes of Genotypes A1 and E (WB and
RT Pig).";
RL Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ESU45082.1, ECO:0000313|Proteomes:UP000018040}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GS {ECO:0000313|EMBL:ESU45082.1,
RC ECO:0000313|Proteomes:UP000018040};
RX PubMed=24307482; DOI=10.1093/gbe/evt197;
RA Adam R.D., Dahlstrom E.W., Martens C.A., Bruno D.P., Barbian K.D.,
RA Ricklefs S.M., Hernandez M.M., Narla N.P., Patel R.B., Porcella S.F.,
RA Nash T.E.;
RT "Genome sequencing of Giardia lamblia genotypes A2 and B isolates (DH and
RT GS) and comparative analysis with the genomes of genotypes A1 and E (WB and
RT Pig).";
RL Genome Biol. Evol. 5:2498-2511(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + FMN + H(+) = diphosphate + FAD; Xref=Rhea:RHEA:17237,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:57692, ChEBI:CHEBI:58210; EC=2.7.7.2;
CC Evidence={ECO:0000256|ARBA:ARBA00001332};
CC -!- PATHWAY: Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step
CC 1/1. {ECO:0000256|ARBA:ARBA00004726}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ESU45082.1}.
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DR EMBL; AHHH01000011; ESU45082.1; -; Genomic_DNA.
DR AlphaFoldDB; V6U372; -.
DR EnsemblProtists; ESU45082; ESU45082; GSB_150799.
DR VEuPathDB; GiardiaDB:DHA2_150166; -.
DR VEuPathDB; GiardiaDB:GL50581_818; -.
DR VEuPathDB; GiardiaDB:GL50803_0016269; -.
DR VEuPathDB; GiardiaDB:QR46_0418; -.
DR Proteomes; UP000018040; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016779; F:nucleotidyltransferase activity; IEA:UniProtKB-KW.
DR CDD; cd01713; PAPS_reductase; 1.
DR Gene3D; 3.40.50.620; HUPs; 1.
DR InterPro; IPR002500; PAPS_reduct.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR PANTHER; PTHR23293:SF9; FAD SYNTHASE; 1.
DR PANTHER; PTHR23293; FAD SYNTHETASE-RELATED FMN ADENYLYLTRANSFERASE; 1.
DR Pfam; PF01507; PAPS_reduct; 1.
DR SUPFAM; SSF52402; Adenine nucleotide alpha hydrolases-like; 1.
PE 4: Predicted;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW FAD {ECO:0000256|ARBA:ARBA00022827};
KW Flavoprotein {ECO:0000256|ARBA:ARBA00022630};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 230..308
FT /note="Phosphoadenosine phosphosulphate reductase"
FT /evidence="ECO:0000259|Pfam:PF01507"
FT REGION 361..384
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 616 AA; 68603 MW; D1C366B7753C8B14 CRC64;
MSSHHIYPEF LFSSPKKEET ILLNPDSVIP SGSEWFPECK SILEFAQKAT NSTNVFIQNR
ISPSSTTVNI SSISHQADCL SSTHSTNVTE HIANNNSTSS TQLSSRLSDS PTTNDALELQ
FAQKINQALI VIRMAMRQYR KLAFSFNGGK DNTVVLYLIR AACLQAVLEE NSDFEGTTPH
ELLRSRFVFF YVHNEVQIKQ VMKFMCLIDH EQNLGTVVYA GTGFKNCIIN FYNDYRPDAV
FMGVRSTDPN GKTTIFSHCS PSWPQFMRVC PILYWSYVDV WDFLKRFRIH YCTLYDQGYT
SLGAATHTIK HPTLRDGTIM HRRIASMVNE PQFSTISGNT HTGKQTVQQI THIFTSKVSL
APHGSNSNPH DRSNQQNWNG SPTIDDKCTS AKVLVKQLMN QAPSVKRPKN EHCGSKTLLL
TNYLSRGVTS ASPTKDTVAN TTTSPSTNLR LKTSASIPTL ESTTDEEIEQ APLSTITPSF
AVDSSNSSMI SQINVQNEYV QQPFVGCEPR TCHRLVSNPD INLSASEEYS ETGSIKSYIG
DTGHEYYGSV TPQDICTYSG PRLVIDELIA RGVPTELLPP QFDPSEESGE IEDDICSASA
IFLPVSEGED ERVNRT
//
