ID V6U4Q0_GIAIN Unreviewed; 813 AA.
AC V6U4Q0;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE RecName: Full=ubiquitinyl hydrolase 1 {ECO:0000256|ARBA:ARBA00012759};
DE EC=3.4.19.12 {ECO:0000256|ARBA:ARBA00012759};
GN ORFNames=GSB_150226 {ECO:0000313|EMBL:ESU45809.1};
OS Giardia intestinalis (Giardia lamblia).
OC Eukaryota; Metamonada; Diplomonadida; Hexamitidae; Giardiinae; Giardia.
OX NCBI_TaxID=5741 {ECO:0000313|EMBL:ESU45809.1, ECO:0000313|Proteomes:UP000018040};
RN [1] {ECO:0000313|Proteomes:UP000018040}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=GS {ECO:0000313|Proteomes:UP000018040};
RA Adam R., Dahlstrom E., Martens C., Bruno D., Barbian K., Porcella S.F.,
RA Nash T.;
RT "Genome sequencing of Giardia lamblia Genotypes A2 and B isolates (DH and
RT GS) and comparative analysis with the genomes of Genotypes A1 and E (WB and
RT Pig).";
RL Submitted (FEB-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ESU45809.1, ECO:0000313|Proteomes:UP000018040}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GS {ECO:0000313|EMBL:ESU45809.1,
RC ECO:0000313|Proteomes:UP000018040};
RX PubMed=24307482; DOI=10.1093/gbe/evt197;
RA Adam R.D., Dahlstrom E.W., Martens C.A., Bruno D.P., Barbian K.D.,
RA Ricklefs S.M., Hernandez M.M., Narla N.P., Patel R.B., Porcella S.F.,
RA Nash T.E.;
RT "Genome sequencing of Giardia lamblia genotypes A2 and B isolates (DH and
RT GS) and comparative analysis with the genomes of genotypes A1 and E (WB and
RT Pig).";
RL Genome Biol. Evol. 5:2498-2511(2013).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC residue protein attached to proteins as an intracellular targeting
CC signal).; EC=3.4.19.12; Evidence={ECO:0000256|ARBA:ARBA00000707};
CC -!- SIMILARITY: Belongs to the peptidase C19 family.
CC {ECO:0000256|ARBA:ARBA00009085}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ESU45809.1}.
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DR EMBL; AHHH01000001; ESU45809.1; -; Genomic_DNA.
DR AlphaFoldDB; V6U4Q0; -.
DR EnsemblProtists; ESU45809; ESU45809; GSB_150226.
DR VEuPathDB; GiardiaDB:DHA2_150247; -.
DR VEuPathDB; GiardiaDB:GL50581_450; -.
DR VEuPathDB; GiardiaDB:GL50803_00102710; -.
DR VEuPathDB; GiardiaDB:QR46_0877; -.
DR Proteomes; UP000018040; Unassembled WGS sequence.
DR GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:UniProtKB-EC.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0019538; P:protein metabolic process; IEA:UniProt.
DR Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR Gene3D; 1.10.8.10; DNA helicase RuvA subunit, C-terminal domain; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 2.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR001394; Peptidase_C19_UCH.
DR InterPro; IPR015940; UBA.
DR InterPro; IPR009060; UBA-like_sf.
DR InterPro; IPR041432; UBP13_Znf-UBP_var.
DR InterPro; IPR018200; USP_CS.
DR InterPro; IPR028889; USP_dom.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR InterPro; IPR001607; Znf_UBP.
DR PANTHER; PTHR24006; UBIQUITIN CARBOXYL-TERMINAL HYDROLASE; 1.
DR PANTHER; PTHR24006:SF758; UBIQUITIN CARBOXYL-TERMINAL HYDROLASE 36; 1.
DR Pfam; PF00443; UCH; 1.
DR Pfam; PF02148; zf-UBP; 1.
DR Pfam; PF17807; zf-UBP_var; 1.
DR SMART; SM00290; ZnF_UBP; 1.
DR SUPFAM; SSF54001; Cysteine proteinases; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR SUPFAM; SSF46934; UBA-like; 1.
DR PROSITE; PS50030; UBA; 1.
DR PROSITE; PS00973; USP_2; 1.
DR PROSITE; PS50235; USP_3; 1.
DR PROSITE; PS50271; ZF_UBP; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000313|EMBL:ESU45809.1};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00502}.
FT DOMAIN 150..269
FT /note="UBP-type"
FT /evidence="ECO:0000259|PROSITE:PS50271"
FT DOMAIN 317..810
FT /note="USP"
FT /evidence="ECO:0000259|PROSITE:PS50235"
FT DOMAIN 633..673
FT /note="UBA"
FT /evidence="ECO:0000259|PROSITE:PS50030"
SQ SEQUENCE 813 AA; 91694 MW; 5E681088075399D9 CRC64;
MIRPCEGYVA STECAYCFRN KYNSQGLYLC MHCNFCTCLD HVNIHQEKTS HMHYLHILYT
LRNKERDITA QDRVDMLLNN GTEEIVDLET KIYQFPGRQA VLSVPDEMMN CYFFLLDGED
NPDRTLLRRK PGMALPSPSN QSSIYDQLAS SCEHVIEVLT GQPHTQQSRD SCFSSCASCD
ISNNLWLCLS CGHVGCGRAQ AYGEMGGNGH ALAHYNSNCD HCVALKLTSL SASVCEAYCY
KCDLSVDAIF TVADMRSMLQ TYLDTFFGHS IASLMTRTEG AGMSLKQQTE RIDELVDRYG
LAKSMQSSQL SLLGVLSGFR NTGNTCYASA VISCLRASGV LPRRTKELEL EALHHFRICT
ASDPINCSVC QLYRLYVWGF RTINYTATDI STHGYLKTDL PYFIMFSDPH VTPLIRLLTP
DFAADENIGF QHDSADYLNS LLDFFRQNGL GPVRTVALPA ARRELRCASC RGHVYKPEDR
QEWGISLTCS LFDTVVECEV GTEVSMQDSL RSELAGYAST IDGLRCPLCS AINVHTEAPK
VLLSSSYLAK NEDDFPEILI VKAIRQYFDV SKMRSYKLLT RLVDANELKI NFLVSTRTDE
DEDMCNTAAW DRAFSTLESG TPQLGTQRDQ ADNIDEQLSV MMDMMIADKE TCVRALRQCS
GDVRLAVDAI LSNEVADDGQ RERCQESLSP QTQTSTLSKD QVVEAVRSLR QEQKVDIEMN
AAGVSYTERD KMNLTYKLVA FVLHRGTELD SGHYLAYVRV DLFSEADLAE MQLSRFVDRD
KDWMVFNDEE VTLTQDPPIQ MAYIYFYRKA HTQ
//
