ID V7AQ28_PHAVU Unreviewed; 387 AA.
AC V7AQ28;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=RING-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012483};
DE EC=2.3.2.27 {ECO:0000256|ARBA:ARBA00012483};
GN ORFNames=PHAVU_010G129300g {ECO:0000313|EMBL:ESW07430.1};
OS Phaseolus vulgaris (Kidney bean) (French bean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Phaseolus.
OX NCBI_TaxID=3885 {ECO:0000313|EMBL:ESW07430.1, ECO:0000313|Proteomes:UP000000226};
RN [1] {ECO:0000313|Proteomes:UP000000226}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. G19833 {ECO:0000313|Proteomes:UP000000226};
RX PubMed=24908249; DOI=10.1038/ng.3008;
RA Schmutz J., McClean P.E., Mamidi S., Wu G.A., Cannon S.B., Grimwood J.,
RA Jenkins J., Shu S., Song Q., Chavarro C., Torres-Torres M., Geffroy V.,
RA Moghaddam S.M., Gao D., Abernathy B., Barry K., Blair M., Brick M.A.,
RA Chovatia M., Gepts P., Goodstein D.M., Gonzales M., Hellsten U.,
RA Hyten D.L., Jia G., Kelly J.D., Kudrna D., Lee R., Richard M.M.,
RA Miklas P.N., Osorno J.M., Rodrigues J., Thareau V., Urrea C.A., Wang M.,
RA Yu Y., Zhang M., Wing R.A., Cregan P.B., Rokhsar D.S., Jackson S.A.;
RT "A reference genome for common bean and genome-wide analysis of dual
RT domestications.";
RL Nat. Genet. 46:707-713(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.27; Evidence={ECO:0000256|ARBA:ARBA00000900};
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DR EMBL; CM002297; ESW07430.1; -; Genomic_DNA.
DR RefSeq; XP_007135436.1; XM_007135374.1.
DR AlphaFoldDB; V7AQ28; -.
DR STRING; 3885.V7AQ28; -.
DR EnsemblPlants; ESW07430; ESW07430; PHAVU_010G129300g.
DR GeneID; 18618288; -.
DR Gramene; ESW07430; ESW07430; PHAVU_010G129300g.
DR KEGG; pvu:PHAVU_010G129300g; -.
DR eggNOG; KOG1571; Eukaryota.
DR OMA; WNRWKEW; -.
DR OrthoDB; 5406876at2759; -.
DR PhylomeDB; V7AQ28; -.
DR Proteomes; UP000000226; Chromosome 10.
DR GO; GO:0009941; C:chloroplast envelope; IEA:EnsemblPlants.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR GO; GO:0006996; P:organelle organization; IEA:InterPro.
DR GO; GO:0016567; P:protein ubiquitination; IEA:InterPro.
DR CDD; cd23145; RING-HC_SPL2-like; 1.
DR Gene3D; 3.30.40.10; Zinc/RING finger domain, C3HC4 (zinc finger); 1.
DR InterPro; IPR022170; MUL1-like.
DR InterPro; IPR044247; SPL2-like.
DR InterPro; IPR001841; Znf_RING.
DR InterPro; IPR013083; Znf_RING/FYVE/PHD.
DR PANTHER; PTHR47355; E3 UBIQUITIN-PROTEIN LIGASE SPL2; 1.
DR PANTHER; PTHR47355:SF1; E3 UBIQUITIN-PROTEIN LIGASE SPL2; 1.
DR Pfam; PF12483; GIDE; 1.
DR Pfam; PF13920; zf-C3HC4_3; 1.
DR SMART; SM00184; RING; 1.
DR SUPFAM; SSF57850; RING/U-box; 1.
DR PROSITE; PS50089; ZF_RING_2; 1.
PE 4: Predicted;
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Reference proteome {ECO:0000313|Proteomes:UP000000226};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786};
KW Zinc {ECO:0000256|ARBA:ARBA00022833};
KW Zinc-finger {ECO:0000256|ARBA:ARBA00022771, ECO:0000256|PROSITE-
KW ProRule:PRU00175}.
FT TRANSMEM 272..293
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 335..375
FT /note="RING-type"
FT /evidence="ECO:0000259|PROSITE:PS50089"
SQ SEQUENCE 387 AA; 42976 MW; FEB12E13179F25A8 CRC64;
MSHQEQALVS LLSQLALSFD GAVLGFALAY AAIRTLVKFS ATSAALRKLR RAPYLSVSDL
RSLLADTPSD ANSNSDGGAI VIVRGNVDAK SAVDGSWKTL RPGVLVSRES GDRGVILQRT
QTCIYNEWKG LFGWTSDLRA IFARSWRQQE STSLRKVPFV LIDVGWRPST EYVVVNMDGS
RHPLPLTTVY HKLQPINASP YTFLQALFGH EYPVGLLDEE KILPVGKDVT AVGLVSLNNG
VAEIKSCKDL PYFLSDLSKD QMIVDLSIKT KILFWGGIAL GSMSVGILGY AVVRNWNKWK
QWKLQRQLQQ QRQAVSDVEP QVDDEIEDVP DGQLCVICLM RRRRSVFIPC GHLVCCQGCA
ISVEHEVAPK CPVCRQEIRN SVRIFES
//
