ID V7B603_PHAVU Unreviewed; 463 AA.
AC V7B603;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 24-JAN-2024, entry version 35.
DE RecName: Full=digalactosyldiacylglycerol synthase {ECO:0000256|ARBA:ARBA00024055};
DE EC=2.4.1.241 {ECO:0000256|ARBA:ARBA00024055};
GN ORFNames=PHAVU_008G069100g {ECO:0000313|EMBL:ESW11906.1};
OS Phaseolus vulgaris (Kidney bean) (French bean).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Fabales; Fabaceae; Papilionoideae; 50 kb inversion clade;
OC NPAAA clade; indigoferoid/millettioid clade; Phaseoleae; Phaseolus.
OX NCBI_TaxID=3885 {ECO:0000313|EMBL:ESW11906.1, ECO:0000313|Proteomes:UP000000226};
RN [1] {ECO:0000313|EMBL:ESW11906.1}
RP NUCLEOTIDE SEQUENCE.
RA Schmutz J., McClean P., Shu S., Cregan P., Rokhsar D., Jackson S.;
RL Submitted (APR-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000000226}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. G19833 {ECO:0000313|Proteomes:UP000000226};
RX PubMed=24908249; DOI=10.1038/ng.3008;
RA Schmutz J., McClean P.E., Mamidi S., Wu G.A., Cannon S.B., Grimwood J.,
RA Jenkins J., Shu S., Song Q., Chavarro C., Torres-Torres M., Geffroy V.,
RA Moghaddam S.M., Gao D., Abernathy B., Barry K., Blair M., Brick M.A.,
RA Chovatia M., Gepts P., Goodstein D.M., Gonzales M., Hellsten U.,
RA Hyten D.L., Jia G., Kelly J.D., Kudrna D., Lee R., Richard M.M.,
RA Miklas P.N., Osorno J.M., Rodrigues J., Thareau V., Urrea C.A., Wang M.,
RA Yu Y., Zhang M., Wing R.A., Cregan P.B., Rokhsar D.S., Jackson S.A.;
RT "A reference genome for common bean and genome-wide analysis of dual
RT domestications.";
RL Nat. Genet. 46:707-713(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-3-O-(beta-D-galactosyl)-sn-glycerol + UDP-alpha-
CC D-galactose = 1,2-diacyl-3-O-[alpha-D-galactosyl-(1->6)-beta-D-
CC galactosyl]-sn-glycerol + H(+) + UDP; Xref=Rhea:RHEA:10520,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:17615, ChEBI:CHEBI:28396,
CC ChEBI:CHEBI:58223, ChEBI:CHEBI:66914; EC=2.4.1.241;
CC Evidence={ECO:0000256|ARBA:ARBA00023914};
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004370}.
CC Plastid, chloroplast outer membrane {ECO:0000256|ARBA:ARBA00024013}.
CC -!- SIMILARITY: Belongs to the glycosyltransferase group 1 family.
CC Glycosyltransferase 4 subfamily. {ECO:0000256|ARBA:ARBA00009481}.
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DR EMBL; CM002295; ESW11906.1; -; Genomic_DNA.
DR EMBL; CM002295; ESW11907.1; -; Genomic_DNA.
DR RefSeq; XP_007139912.1; XM_007139850.1.
DR RefSeq; XP_007139913.1; XM_007139851.1.
DR AlphaFoldDB; V7B603; -.
DR STRING; 3885.V7B603; -.
DR EnsemblPlants; ESW11906; ESW11906; PHAVU_008G069100g.
DR EnsemblPlants; ESW11907; ESW11907; PHAVU_008G069100g.
DR GeneID; 18622084; -.
DR Gramene; ESW11906; ESW11906; PHAVU_008G069100g.
DR Gramene; ESW11907; ESW11907; PHAVU_008G069100g.
DR KEGG; pvu:PHAVU_008G069100g; -.
DR eggNOG; ENOG502QQEJ; Eukaryota.
DR OMA; FHHGKKW; -.
DR OrthoDB; 276624at2759; -.
DR Proteomes; UP000000226; Chromosome 8.
DR GO; GO:0009707; C:chloroplast outer membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0046481; F:digalactosyldiacylglycerol synthase activity; IEA:UniProtKB-EC.
DR CDD; cd01635; Glycosyltransferase_GTB-type; 1.
DR Gene3D; 3.40.50.2000; Glycogen Phosphorylase B; 2.
DR InterPro; IPR044525; DGDG1/2.
DR InterPro; IPR001296; Glyco_trans_1.
DR PANTHER; PTHR46132; DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTIC; 1.
DR PANTHER; PTHR46132:SF1; DIGALACTOSYLDIACYLGLYCEROL SYNTHASE 2, CHLOROPLASTIC; 1.
DR Pfam; PF00534; Glycos_transf_1; 1.
DR SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE 3: Inferred from homology;
KW Glycosyltransferase {ECO:0000256|ARBA:ARBA00022676};
KW Membrane {ECO:0000256|ARBA:ARBA00022805};
KW Plastid {ECO:0000256|ARBA:ARBA00022805};
KW Plastid outer membrane {ECO:0000256|ARBA:ARBA00022805};
KW Reference proteome {ECO:0000313|Proteomes:UP000000226};
KW Transferase {ECO:0000256|ARBA:ARBA00022676}.
FT DOMAIN 216..335
FT /note="Glycosyl transferase family 1"
FT /evidence="ECO:0000259|Pfam:PF00534"
SQ SEQUENCE 463 AA; 52648 MW; 46B85153B7959FCB CRC64;
MDKKEHIAIF TTASLPWLTG TAVNPLFRAA YLCKSGERDV TLVIPWLSLK DQGLVYPNKI
TFASPNEHEK YIRKWLEERV GFSSGFSIQF YPGKFSIEKR SILAVGDISE IIPDEVADIA
VLEEPEHLTW YHHGKRWKTK FRLVIGIIHT NYLEYVKREK NGIMQAFLLK YLNSWVVGIY
CHKVIRLSAA TQDYTESIIC NVHGVNPKFL EIGEKKREQQ QKGIKAFTKG AYFIGKMIWS
KGYRELLQLL KDHKKELSAL EVDLFGSGED SDEVHKAAEK LQLTVRLNPA RDHADALFHD
YKLFLNPSTT DVVCTTTAET LAMGKIVVCA NHPSNQFFKQ FTNCWTYDNS EEFVRLTLKA
LAEEPAQLTD AERHELSWDA ATERFLKAVG LDKPSEKKLS RSSSNFMAAS INLQQTVDEA
SAFVHHVVSG FEVSRRIFGA IPDSLEPDEE ISKELGLTNA VRK
//
