ID V7HX07_9LACO Unreviewed; 693 AA.
AC V7HX07;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 40.
DE RecName: Full=Glycine--tRNA ligase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
DE EC=6.1.1.14 {ECO:0000256|HAMAP-Rule:MF_00255};
DE AltName: Full=Glycyl-tRNA synthetase beta subunit {ECO:0000256|HAMAP-Rule:MF_00255};
DE Short=GlyRS {ECO:0000256|HAMAP-Rule:MF_00255};
GN Name=glyS {ECO:0000256|HAMAP-Rule:MF_00255};
GN ORFNames=LEQ_1867c {ECO:0000313|EMBL:ETA74407.1};
OS Ligilactobacillus equi DPC 6820.
OC Bacteria; Bacillota; Bacilli; Lactobacillales; Lactobacillaceae;
OC Ligilactobacillus.
OX NCBI_TaxID=1392007 {ECO:0000313|EMBL:ETA74407.1, ECO:0000313|Proteomes:UP000018559};
RN [1] {ECO:0000313|EMBL:ETA74407.1, ECO:0000313|Proteomes:UP000018559}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DPC 6820 {ECO:0000313|EMBL:ETA74407.1,
RC ECO:0000313|Proteomes:UP000018559};
RX PubMed=24435863;
RA O'Donnell M.M., Harris H.M., O'Toole P.W., Ross R.P.;
RT "The Genome of the Predominant Equine Lactobacillus Species, Lactobacillus
RT equi, Is Reflective of Its Lifestyle Adaptations to an Herbivorous Host.";
RL Genome Announc. 2:e01155-e01113(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + glycine + tRNA(Gly) = AMP + diphosphate + glycyl-
CC tRNA(Gly); Xref=Rhea:RHEA:16013, Rhea:RHEA-COMP:9664, Rhea:RHEA-
CC COMP:9683, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57305,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78522, ChEBI:CHEBI:456215;
CC EC=6.1.1.14; Evidence={ECO:0000256|ARBA:ARBA00000201,
CC ECO:0000256|HAMAP-Rule:MF_00255};
CC -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC {ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|ARBA:ARBA00004496,
CC ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00008226, ECO:0000256|HAMAP-Rule:MF_00255}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ETA74407.1}.
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DR EMBL; AWWH01000074; ETA74407.1; -; Genomic_DNA.
DR RefSeq; WP_023859382.1; NZ_AWWH01000074.1.
DR AlphaFoldDB; V7HX07; -.
DR PATRIC; fig|1392007.3.peg.777; -.
DR Proteomes; UP000018559; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004820; F:glycine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:InterPro.
DR GO; GO:0006426; P:glycyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR HAMAP; MF_00255; Gly_tRNA_synth_beta; 1.
DR InterPro; IPR008909; DALR_anticod-bd.
DR InterPro; IPR015944; Gly-tRNA-synth_bsu.
DR InterPro; IPR006194; Gly-tRNA-synth_heterodimer.
DR NCBIfam; TIGR00211; glyS; 1.
DR PANTHER; PTHR30075:SF2; GLYCINE--TRNA LIGASE, CHLOROPLASTIC_MITOCHONDRIAL 2; 1.
DR PANTHER; PTHR30075; GLYCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF05746; DALR_1; 1.
DR Pfam; PF02092; tRNA_synt_2f; 1.
DR PRINTS; PR01045; TRNASYNTHGB.
DR SUPFAM; SSF109604; HD-domain/PDEase-like; 1.
DR PROSITE; PS50861; AA_TRNA_LIGASE_II_GLYAB; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146,
KW ECO:0000256|HAMAP-Rule:MF_00255};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|HAMAP-
KW Rule:MF_00255}; Cytoplasm {ECO:0000256|HAMAP-Rule:MF_00255};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000256|HAMAP-Rule:MF_00255};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|HAMAP-
KW Rule:MF_00255};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917, ECO:0000256|HAMAP-
KW Rule:MF_00255}; Reference proteome {ECO:0000313|Proteomes:UP000018559}.
FT DOMAIN 586..683
FT /note="DALR anticodon binding"
FT /evidence="ECO:0000259|Pfam:PF05746"
SQ SEQUENCE 693 AA; 76932 MW; F846A8F91D7E0DA8 CRC64;
MAHTFLLEIG LEEIPAHVVT PSVNQLVAKV ENYLKEERLA FDSVLPFSTP RRLAVKVTGL
ADKQEDISTE AKGPAKKIAQ DAEGNWTKAA QGFARGQGLT TDDLFFKELK GVEYVYANKF
EAGKSALEVL AGMKEVAMSL KFPTMMRWGT NDFEYVRPIR WLVALLDNEV VNFDILGTKT
GRVSQGHRFL GKEVTLNSTD DYPEALLAES VIADATKRKN MIRGQINDLA QENGWKVDID
EDLLEEVNNL VEWPTVFAGN FDEKYLAVPD VVLITSMKDN QRYFYVTDAA GKLLPHFIAI
RNGKDAFLNN VIAGNEKVLT ARLEDAKFFF EEDQKQSLQH YVDRLDKVMF HDKIGTLAQK
MQRVGLLAQF LGQKLGLSDQ ELADLARASQ IYKFDLVTGM VGEFSELQGV MGEIYANLMG
ENAVVAQAIR EEYMPTSAEG VLPASTVGSV LSIADKLDSL QAFFSAGMVP SGSNDPYALR
RQTLGIIRIA LANNWQLSVD DLMDAVAFAQ GKAHDLYANI DPATNAQALR DFVADRLHQI
LANDHYRHDV LETVVANMAN TFVDVSQAAK TLTAHLDDAD FKETIEALTR VARLAAKADL
SQTLTVDESL FENDSEKALF AAYQVQKEAM EAANDYDAKY QAIHALKDAI SAYFEATMVM
VEDTAVKNNR LHQLVLVKQL TQNFGDLAQL NVK
//
