ID V7I080_9CLOT Unreviewed; 314 AA.
AC V7I080;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 43.
DE SubName: Full=N-acetylneuraminate lyase {ECO:0000313|EMBL:ETA79298.1};
GN ORFNames=T472_0217765 {ECO:0000313|EMBL:ETA79298.1};
OS Youngiibacter fragilis 232.1.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Clostridiaceae;
OC Youngiibacter.
OX NCBI_TaxID=994573 {ECO:0000313|EMBL:ETA79298.1, ECO:0000313|Proteomes:UP000017747};
RN [1] {ECO:0000313|EMBL:ETA79298.1, ECO:0000313|Proteomes:UP000017747}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=232.1 {ECO:0000313|EMBL:ETA79298.1,
RC ECO:0000313|Proteomes:UP000017747};
RX PubMed=24459265;
RA Wawrik C.B., Callaghan A.V., Stamps B.W., Wawrik B.;
RT "Genome Sequence of Youngiibacter fragilis, the Type Strain of the Genus
RT Youngiibacter.";
RL Genome Announc. 2:e01183-13(2014).
CC -!- SIMILARITY: Belongs to the DapA family.
CC {ECO:0000256|PIRNR:PIRNR001365}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ETA79298.1}.
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DR EMBL; AXUN02000219; ETA79298.1; -; Genomic_DNA.
DR RefSeq; WP_023384072.1; NZ_AXUN02000219.1.
DR AlphaFoldDB; V7I080; -.
DR STRING; 994573.T472_0217765; -.
DR eggNOG; COG0329; Bacteria.
DR OrthoDB; 9782828at2; -.
DR Proteomes; UP000017747; Unassembled WGS sequence.
DR GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR002220; DapA-like.
DR InterPro; IPR020624; Schiff_base-form_aldolases_CS.
DR PANTHER; PTHR42849; N-ACETYLNEURAMINATE LYASE; 1.
DR PANTHER; PTHR42849:SF1; N-ACETYLNEURAMINATE LYASE; 1.
DR Pfam; PF00701; DHDPS; 1.
DR PIRSF; PIRSF001365; DHDPS; 1.
DR PRINTS; PR00146; DHPICSNTHASE.
DR SMART; SM01130; DHDPS; 1.
DR SUPFAM; SSF51569; Aldolase; 1.
DR PROSITE; PS00665; DHDPS_1; 1.
PE 3: Inferred from homology;
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|PIRNR:PIRNR001365};
KW Reference proteome {ECO:0000313|Proteomes:UP000017747}.
FT ACT_SITE 140
FT /note="Proton donor/acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001365-1"
FT ACT_SITE 166
FT /note="Schiff-base intermediate with substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR001365-1"
FT BINDING 52
FT /ligand="pyruvate"
FT /ligand_id="ChEBI:CHEBI:15361"
FT /evidence="ECO:0000256|PIRSR:PIRSR001365-2"
FT BINDING 208
FT /ligand="pyruvate"
FT /ligand_id="ChEBI:CHEBI:15361"
FT /evidence="ECO:0000256|PIRSR:PIRSR001365-2"
SQ SEQUENCE 314 AA; 34265 MW; 49908D13F2EECF31 CRC64;
MANFNISEIK GVIPALLTPF TAEEELDEKG LRALISHLIG EGVHGLYLVG STGEGFLMSQ
DERKKAVEIA MDEVKGRIPV IVHVGAISTR LSKELTVHAY ESGADAVSSV PPFYYKFSEE
QIIGYYRDLA EASPLPMIIY NIPLAGIMGY GTLVKLAKIP GVKGIKYTST THFEITQLKS
DIGADFMVYS GADEMALSGL LAGSDGIIGS TYNAIPDTFL AIYDAFVKGD IERANNVMKQ
GVEVIMQMLK YGSLMSLLKA INRWAGVPAG YARKPFSNFT EAEEASIRKD FVALKKRLSL
TDVKFIDTIE KNEA
//
