ID V8CCB9_9HELI Unreviewed; 486 AA.
AC V8CCB9;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=Nickel/cobalt efflux system {ECO:0000256|RuleBase:RU362101};
GN ORFNames=HMPREF2086_00383 {ECO:0000313|EMBL:ETD25048.1};
OS Helicobacter macacae MIT 99-5501.
OC Bacteria; Campylobacterota; Epsilonproteobacteria; Campylobacterales;
OC Helicobacteraceae; Helicobacter.
OX NCBI_TaxID=1357400 {ECO:0000313|EMBL:ETD25048.1, ECO:0000313|Proteomes:UP000018731};
RN [1] {ECO:0000313|EMBL:ETD25048.1, ECO:0000313|Proteomes:UP000018731}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MIT 99-5501 {ECO:0000313|EMBL:ETD25048.1,
RC ECO:0000313|Proteomes:UP000018731};
RX PubMed=25212613;
RA Shen Z., Sheh A., Young S.K., Abouelliel A., Ward D.V., Earl A.M.,
RA Fox J.G.;
RT "Draft genome sequences of six enterohepatic helicobacter species isolated
RT from humans and one from rhesus macaques.";
RL Genome Announc. 2:e00857-e00814(2014).
CC -!- FUNCTION: Efflux system for nickel and cobalt.
CC {ECO:0000256|ARBA:ARBA00002510}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000256|RuleBase:RU362101};
CC Multi-pass membrane protein {ECO:0000256|RuleBase:RU362101}. Membrane
CC {ECO:0000256|ARBA:ARBA00004141}; Multi-pass membrane protein
CC {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the NiCoT transporter (TC 2.A.52) family.
CC {ECO:0000256|RuleBase:RU362101}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ETD25048.1}.
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DR EMBL; AZJI01000001; ETD25048.1; -; Genomic_DNA.
DR RefSeq; WP_023927063.1; NZ_KI669454.1.
DR AlphaFoldDB; V8CCB9; -.
DR STRING; 1357400.HMPREF2086_00383; -.
DR PATRIC; fig|1357400.3.peg.527; -.
DR eggNOG; COG2215; Bacteria.
DR HOGENOM; CLU_573339_0_0_7; -.
DR OrthoDB; 9812956at2; -.
DR Proteomes; UP000018731; Unassembled WGS sequence.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0015099; F:nickel cation transmembrane transporter activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006824; P:cobalt ion transport; IEA:UniProtKB-KW.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR011541; Ni/Co_transpt_high_affinity.
DR PANTHER; PTHR40659; NICKEL/COBALT EFFLUX SYSTEM RCNA; 1.
DR PANTHER; PTHR40659:SF1; NICKEL_COBALT EFFLUX SYSTEM RCNA; 1.
DR Pfam; PF03824; NicO; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
DR PROSITE; PS50222; EF_HAND_2; 1.
PE 3: Inferred from homology;
KW Cell membrane {ECO:0000256|ARBA:ARBA00022475};
KW Cobalt {ECO:0000256|ARBA:ARBA00023285};
KW Cobalt transport {ECO:0000256|ARBA:ARBA00022426};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|RuleBase:RU362101};
KW Nickel {ECO:0000256|ARBA:ARBA00022596};
KW Nickel transport {ECO:0000256|ARBA:ARBA00023112};
KW Reference proteome {ECO:0000313|Proteomes:UP000018731};
KW Signal {ECO:0000256|SAM:SignalP};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692,
KW ECO:0000256|RuleBase:RU362101};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|RuleBase:RU362101};
KW Transport {ECO:0000256|ARBA:ARBA00022448, ECO:0000256|RuleBase:RU362101}.
FT SIGNAL 1..19
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 20..486
FT /note="Nickel/cobalt efflux system"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004767496"
FT TRANSMEM 257..281
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 302..324
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 336..357
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 394..417
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 423..446
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT TRANSMEM 466..485
FT /note="Helical"
FT /evidence="ECO:0000256|RuleBase:RU362101"
FT DOMAIN 57..92
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
SQ SEQUENCE 486 AA; 54407 MW; 7E265AD2ECC592D8 CRC64;
MWRFIFCFGL AISKIYSCAL CMAYIPNVVV NVISIDFDET SKGGKKPNAI VAFEWEFEKE
FNQTMFHTYD KNANGVIEPS EITQIKQSLT SYILPRAQLT KALIKQGDKL ERAKFTLQSD
EMFIRDTLLI YRYKIKLPLQ NANTFSFQVN DNEGFFNFTF SQHKKTFMLN ERLWASNTID
NVIIFREQET KGLESKNLDS SQNPNTQNLA SDFAKQSQQT FWQNLRSDLW QKIATLNAKI
LSNIKSYIYD EQGKTSFFSI LFLVAISFGY GVFHAAAPGH AKLLTSSYFL SHKSSYSKAF
SFALKVGVVH ILSAFLLVGL GLFVLKVVVQ SLANQANIII TQVSSVIIVL LACILLVRKM
RHSSACECKT CNLSKSASSD KSNEPKNQAK RFDFAQWSAI IAAGIVPCPT MIIVLLLCFE
AGFFSALLSA ICIALGMSAV VFISAIFAHK VRLSATSQTI SRALEYFALI CMLCLGIFMF
ANAGVL
//
