ID V9FG21_PHYPR Unreviewed; 479 AA.
AC V9FG21;
DT 19-FEB-2014, integrated into UniProtKB/TrEMBL.
DT 19-FEB-2014, sequence version 1.
DT 24-JAN-2024, entry version 27.
DE RecName: Full=malate synthase {ECO:0000256|ARBA:ARBA00012636};
DE EC=2.3.3.9 {ECO:0000256|ARBA:ARBA00012636};
GN ORFNames=F443_06030 {ECO:0000313|EMBL:ETI50409.1};
OS Phytophthora parasitica P1569.
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=1317065 {ECO:0000313|EMBL:ETI50409.1, ECO:0000313|Proteomes:UP000018721};
RN [1] {ECO:0000313|EMBL:ETI50409.1, ECO:0000313|Proteomes:UP000018721}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=P1569 {ECO:0000313|EMBL:ETI50409.1,
RC ECO:0000313|Proteomes:UP000018721};
RG The Broad Institute Genomics Platform;
RA Russ C., Tyler B., Panabieres F., Shan W., Tripathy S., Grunwald N.,
RA Machado M., Johnson C.S., Arredondo F., Hong C., Coffey M., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Abouelleil A., Alvarado L.,
RA Chapman S.B., Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Ireland A., Larimer J., McCowan C., Murphy C.,
RA Pearson M., Poon T.W., Priest M., Roberts A., Saif S., Shea T., Sykes S.,
RA Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Phytophthora parasitica P1569.";
RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00001699};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ETI50409.1}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; ANIZ01001028; ETI50409.1; -; Genomic_DNA.
DR AlphaFoldDB; V9FG21; -.
DR EnsemblProtists; ETI50409; ETI50409; F443_06030.
DR HOGENOM; CLU_018928_3_0_1; -.
DR OrthoDB; 177378at2759; -.
DR Proteomes; UP000018721; Unassembled WGS sequence.
DR GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR Gene3D; 3.20.20.360; Malate synthase, domain 3; 1.
DR Gene3D; 1.20.1220.12; Malate synthase, domain III; 1.
DR InterPro; IPR044856; Malate_synth_C_sf.
DR InterPro; IPR011076; Malate_synth_sf.
DR InterPro; IPR006252; Malate_synthA.
DR InterPro; IPR001465; Malate_synthase_TIM.
DR InterPro; IPR048355; MS_C.
DR InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR PANTHER; PTHR42902; MALATE SYNTHASE; 1.
DR PANTHER; PTHR42902:SF2; MALATE SYNTHASE; 1.
DR Pfam; PF20659; MS_C; 1.
DR Pfam; PF01274; MS_TIM-barrel; 1.
DR SUPFAM; SSF51645; Malate synthase G; 1.
PE 4: Predicted;
KW Glyoxylate bypass {ECO:0000256|ARBA:ARBA00022435};
KW Reference proteome {ECO:0000313|Proteomes:UP000018721};
KW Transferase {ECO:0000256|ARBA:ARBA00022679};
KW Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532}.
FT DOMAIN 99..320
FT /note="Malate synthase TIM barrel"
FT /evidence="ECO:0000259|Pfam:PF01274"
FT DOMAIN 353..445
FT /note="Malate synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF20659"
FT ACT_SITE 102
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR601465-50"
FT ACT_SITE 386
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR601465-50"
SQ SEQUENCE 479 AA; 54099 MW; DDB8A4512C21F964 CRC64;
MPHFLEETRA IREDETWRVD PQPNALMDRR VDIGDVSPAN RKFLLRALNS GAQGVQVDFD
DGHCPTWSNT LQGHFNVLQA ARGLLEVSGQ TTVANPALLV IRPRAWNMDE PHMVVNGRVV
PGALFDFGMH LFHNGKHLLN NGTGPFLYLP KLEGYKEAAL WRRIFEYTEK KLEFARGRIK
ATVLIENIFA AFEMDEILFE LRHYSSGLNC GMWDYTASIV VNFRHRPAFL LPDRQQFVSM
KSDFLRYYMD LLIATCKRRH APATTGMVPF VLAELPPGFS QAEAIEKTRT GKTMEALAGS
DGALVYDLAL VKPGAEVFTS ARERTTKAGF SYVAVDERLF TEKLLTLPKG DVTLQSVEMN
VHVALLYVLH WLYGQGTVVV NGCVEDSATA EISRAQLWQW VHHRVTITGT PQQINASLIG
KILRKLCHEE NRDQRHPPQY MEVAQHLVLL LSTMRSPPAF ITTFLQEQEA LTKALNTTK
//
