ID V9NCA3_MANES Unreviewed; 449 AA.
AC V9NCA3;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 24-JAN-2024, entry version 42.
DE RecName: Full=Purple acid phosphatase {ECO:0000256|RuleBase:RU361203};
DE EC=3.1.3.2 {ECO:0000256|RuleBase:RU361203};
GN Name=PAP22 {ECO:0000313|EMBL:AGL44400.1};
GN ORFNames=MANES_08G024000 {ECO:0000313|EMBL:OAY42888.1};
OS Manihot esculenta (Cassava) (Jatropha manihot).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; fabids; Malpighiales; Euphorbiaceae; Crotonoideae; Manihoteae;
OC Manihot.
OX NCBI_TaxID=3983 {ECO:0000313|EMBL:AGL44400.1};
RN [1] {ECO:0000313|EMBL:AGL44400.1}
RP NUCLEOTIDE SEQUENCE.
RA Wang H., Zhang P.;
RT "Identification of cassava purple acid phosphatases related to phosphate
RT starvation signalling.";
RL Submitted (JUL-2012) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:OAY42888.1, ECO:0000313|Proteomes:UP000091857}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. AM560-2 {ECO:0000313|Proteomes:UP000091857};
RC TISSUE=Leaf {ECO:0000313|EMBL:OAY42888.1};
RA Bredeson J.V., Prochnik S.E., Lyons J.B., Schmutz J., Grimwood J.,
RA Vrebalov J., Bart R.S., Amuge T., Ferguson M.E., Green R., Putnam N.,
RA Stites J., Rounsley S., Rokhsar D.S.;
RT "WGS assembly of Manihot esculenta.";
RL Submitted (FEB-2016) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a phosphate monoester + H2O = an alcohol + phosphate;
CC Xref=Rhea:RHEA:15017, ChEBI:CHEBI:15377, ChEBI:CHEBI:30879,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:67140; EC=3.1.3.2;
CC Evidence={ECO:0000256|ARBA:ARBA00000032,
CC ECO:0000256|RuleBase:RU361203};
CC -!- COFACTOR:
CC Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC Evidence={ECO:0000256|ARBA:ARBA00001962};
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- SIMILARITY: Belongs to the metallophosphoesterase superfamily. Purple
CC acid phosphatase family. {ECO:0000256|ARBA:ARBA00008723,
CC ECO:0000256|RuleBase:RU361203}.
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DR EMBL; JX402070; AGL44400.1; -; mRNA.
DR EMBL; CM004394; OAY42888.1; -; Genomic_DNA.
DR AlphaFoldDB; V9NCA3; -.
DR STRING; 3983.V9NCA3; -.
DR EnsemblPlants; OAY42888; OAY42888; MANES_08G024000.
DR Gramene; OAY42888; OAY42888; MANES_08G024000.
DR OMA; SAGCHTP; -.
DR OrthoDB; 5485592at2759; -.
DR Proteomes; UP000091857; Chromosome lg8.
DR GO; GO:0003993; F:acid phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:InterPro.
DR CDD; cd00839; MPP_PAPs; 1.
DR Gene3D; 3.60.21.10; -; 1.
DR Gene3D; 2.60.40.380; Purple acid phosphatase-like, N-terminal; 1.
DR InterPro; IPR004843; Calcineurin-like_PHP_ApaH.
DR InterPro; IPR029052; Metallo-depent_PP-like.
DR InterPro; IPR041792; MPP_PAP.
DR InterPro; IPR039331; PPA-like.
DR InterPro; IPR008963; Purple_acid_Pase-like_N.
DR InterPro; IPR015914; Purple_acid_Pase_N.
DR InterPro; IPR025733; Purple_acid_PPase_C_dom.
DR PANTHER; PTHR22953; ACID PHOSPHATASE RELATED; 1.
DR PANTHER; PTHR22953:SF7; PURPLE ACID PHOSPHATASE 22; 1.
DR Pfam; PF00149; Metallophos; 1.
DR Pfam; PF14008; Metallophos_C; 1.
DR Pfam; PF16656; Pur_ac_phosph_N; 1.
DR SUPFAM; SSF56300; Metallo-dependent phosphatases; 1.
DR SUPFAM; SSF49363; Purple acid phosphatase, N-terminal domain; 1.
PE 2: Evidence at transcript level;
KW Glycoprotein {ECO:0000256|ARBA:ARBA00023180};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU361203};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Reference proteome {ECO:0000313|Proteomes:UP000091857};
KW Signal {ECO:0000256|ARBA:ARBA00022729, ECO:0000256|RuleBase:RU361203};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|RuleBase:RU361203"
FT CHAIN 21..449
FT /note="Purple acid phosphatase"
FT /evidence="ECO:0000256|RuleBase:RU361203"
FT /id="PRO_5011327442"
FT DOMAIN 53..140
FT /note="Purple acid phosphatase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16656"
FT DOMAIN 149..339
FT /note="Calcineurin-like phosphoesterase"
FT /evidence="ECO:0000259|Pfam:PF00149"
FT DOMAIN 354..413
FT /note="Iron/zinc purple acid phosphatase-like C-terminal"
FT /evidence="ECO:0000259|Pfam:PF14008"
SQ SEQUENCE 449 AA; 50935 MW; 66C1DCEF9C913519 CRC64;
MEKLFPFGLS LLLATTSVFP HFPFLSHASE NVYSRPSARS VIFTRHKGSE SEPQQVHISL
VGKDHMRVTW ITDDKHVESR VEYGKQPGKY NAIATGEQTS YRYFFYSSGK IHHVKIGPLE
SATTYYYRCG GYGPEFSFKT PPSAFPIEFA VVGDLGQTEW TKSTLEHVGS RDYDVFLLPG
DLSYADSHQP LWDSFGRLVE PYATQRPWMV TEGNHEIETV PIIFPHGFKA YNARWRMPYE
ESGSASNLYY SFEVGGVHVI MLGSYTDFAS DSDQYKWLVG DLGGVDRKKT PWVVVLLHAP
WYNTNLAHQG EGEEMRKAME DLLYEARVDV VFAGHVHAYE RFTRIYNNKA DPCGPMYITI
GDGGNREGLA LTFKNPGSSL SLYREPSFGH GRLRILDEKR AHWSWQRNND SDSVEADEVW
LDNLTTSKAC WASVDAQQQQ PSSSANEEL
//
