ID VP13F_DICDI Reviewed; 4246 AA.
AC Q54KX3;
DT 03-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 24-MAY-2005, sequence version 1.
DT 27-MAR-2024, entry version 85.
DE RecName: Full=Intermembrane lipid transfer protein vps13F {ECO:0000250|UniProtKB:Q96RL7};
DE AltName: Full=Putative vacuolar protein sorting-associated protein 13F;
GN Name=vps13F; ORFNames=DDB_G0287055;
OS Dictyostelium discoideum (Social amoeba).
OC Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC Dictyosteliaceae; Dictyostelium.
OX NCBI_TaxID=44689;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=AX4;
RX PubMed=15875012; DOI=10.1038/nature03481;
RA Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT "The genome of the social amoeba Dictyostelium discoideum.";
RL Nature 435:43-57(2005).
CC -!- FUNCTION: Mediates the transfer of lipids between membranes at
CC organelle contact sites. {ECO:0000250|UniProtKB:Q07878}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Peripheral membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the VPS13 family. {ECO:0000305}.
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DR EMBL; AAFI02000096; EAL63892.1; -; Genomic_DNA.
DR RefSeq; XP_637397.1; XM_632305.1.
DR SMR; Q54KX3; -.
DR STRING; 44689.Q54KX3; -.
DR PaxDb; 44689-DDB0304549; -.
DR ABCD; Q54KX3; 5 sequenced antibodies.
DR EnsemblProtists; EAL63892; EAL63892; DDB_G0287055.
DR GeneID; 8625928; -.
DR KEGG; ddi:DDB_G0287055; -.
DR dictyBase; DDB_G0287055; vps13F.
DR eggNOG; KOG1809; Eukaryota.
DR HOGENOM; CLU_223857_0_0_1; -.
DR InParanoid; Q54KX3; -.
DR OMA; GPTNILR; -.
DR PhylomeDB; Q54KX3; -.
DR PRO; PR:Q54KX3; -.
DR Proteomes; UP000002195; Chromosome 4.
DR GO; GO:0019898; C:extrinsic component of membrane; IBA:GO_Central.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; IMP:dictyBase.
DR GO; GO:0016045; P:detection of bacterium; IMP:dictyBase.
DR GO; GO:0045053; P:protein retention in Golgi apparatus; IBA:GO_Central.
DR GO; GO:0006623; P:protein targeting to vacuole; IBA:GO_Central.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR GO; GO:0051593; P:response to folic acid; IMP:dictyBase.
DR InterPro; IPR015412; Atg2/VPS13_C.
DR InterPro; IPR026847; VPS13.
DR InterPro; IPR026854; VPS13-like_N.
DR InterPro; IPR049424; VPS13_C.
DR InterPro; IPR031645; VPS13_DH-like.
DR InterPro; IPR031646; VPS13_extend_chorein.
DR InterPro; IPR031642; VPS13_mid_RBG.
DR PANTHER; PTHR16166:SF97; INTERMEMBRANE LIPID TRANSFER PROTEIN VPS13F; 1.
DR PANTHER; PTHR16166; VACUOLAR PROTEIN SORTING-ASSOCIATED PROTEIN VPS13; 1.
DR Pfam; PF09333; ATG2-VPS13_C; 1.
DR Pfam; PF12624; Chorein_N; 1.
DR Pfam; PF21679; VPS13_C; 1.
DR Pfam; PF16909; VPS13_DH-like; 1.
DR Pfam; PF16908; VPS13_ext_chorein; 1.
DR Pfam; PF16910; VPS13_mid_rpt; 3.
DR SUPFAM; SSF81995; beta-sandwich domain of Sec23/24; 1.
PE 3: Inferred from homology;
KW Membrane; Protein transport; Reference proteome; Transport.
FT CHAIN 1..4246
FT /note="Intermembrane lipid transfer protein vps13F"
FT /id="PRO_0000366144"
FT DOMAIN 2..113
FT /note="Chorein N-terminal"
FT /evidence="ECO:0000255"
FT REGION 141..271
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 401..420
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 591..759
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 914..944
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 964..1014
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1217..1251
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1356..1379
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1395..1436
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1622..1683
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2101..2131
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2211..2237
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2471..2513
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2704..2756
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3421..3449
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3611..3652
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 3794..3813
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 168..203
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 218..271
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 591..681
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 682..704
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 735..759
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 925..944
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 981..1009
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1361..1379
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1395..1414
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1415..1433
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1622..1636
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1637..1683
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3430..3449
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 3794..3809
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 4246 AA; 481243 MW; E9158B5661097DA3 CRC64;
MFESIVSNLL TRYLKEYIKA LNTDNLNISI WSGNVSLENL ELKSRALEKF NLPFTIKEGF
LGKLSLKIPW NNLKTEPVIV VIDQLYITAS PKSTTVWNEE DEDLLLQKKL KKLKIYEALK
EEKRKKQQQL KEKREIRNNL IKEKRLQKKQ IKNQLKQQLR QRKKDAKNAS PVNSNNNNNN
NSNLVSESNI PSSSSSSSSS LYDDDDNSSK DANKSDDTDM DVDDDDEFQE ATEGDYDNEE
EQDDHDEEDD LSDDDDDDDD EEDDYEMEDE DDVYKTLVDN QGNENDSQSG GLIDSSSFAE
KLRNKIVDNL QIMIYNVHIR FEDSVTNPTT PYCFGVTLEH LLAQSSNEEW KPSFIHTPHT
LVHKLITLQN LSIYWDADND KPDLNYKSIS EFKSLMTKLI PKKSSPSTTT TTPPISPPPK
ETQLIKPQHN YLLHPVCGSL KLLINKSIIP NKYIPLYTAN FEFDEINLEL ESKQYSGILA
LVDWFTTQKK GERYRKFKTK ESGGVLKTAK DRWEFAGKCI LADVRDKIRK WTPQFFDKRR
KDRLDYIHLY KLKKRKKTLS DLNTTKLNSL EKEYSFEDLV YFRSLADAQL KAEDNHQQQQ
KQQQQQQQQQ SNISPKIISS NKNNNNDSSS GSSSNNNNSS TTSTTSTTTS SSSSSNSTDS
KDIMKSSGDK NVNNNNNMGD NENKDNIDKK EENKNDDQDN KNKNKNKIIT TISHKKNKSK
DDQNSGGWFK WFGWGSSNKS KQQQQQQDES EDQDSSILGT LDITDQQKRE LYSTIEYDEH
QKSKNIVYPK DYVKTRINFT MNRGSVAFRL QPPNSPITHF SSKSPPSSKG NADLMIELVR
MSVAVDKFVG SLYVQAILET IYVRDFFTKD TQFPILMKPI VVKNNTFVNS SSRTLPYSNS
EGVEDINKDY LSIVSSSPSP VSSPSRDKRL EEETKQEDEK EKKLNVDKNL KCSLSSIEKV
SCDKKSKDDC SSGGSGGVDN GGEEGDDKYS TKGTTESREE SDGSGDYENN TNDEKQPLFS
MTLQQNPLNS ESDFSIFIEA LPLEVVYNKS MMDAILEFFG NAPSDTLKEI EEAARNQIKI
FKDKTTLRIQ HELENHKSID LDININAPHV LIPESFTIPS APILVLDLGS FSVQSVVTHK
DDTLSEEIEI SSILKNDFSI IQQQQQQQQQ QQQQQQQQQQ QQQQQQQQQQ QIPIQPQSQQ
QQQQQQQQQQ QSQQQQQAQQ QAQQQQQSQH PSSNDDNSSS NGGCSVDIKS PSVNTSIISD
NSVYDEENVT KYLYDLFELK LNNIQFYIAS QGESLQLVEK FDINFKIYKC IIKSQTLLSK
LKLFGGLPSL NMLLSQKSIE ILINIISTLQ NDPPEISTHF SSKKNAEDEN NNSNLDRVDF
ETVIHDQEVD IEEYYNGVNS DDDNNDDEND KTTQDDQDDK EKSSGNDDSL KKKSKKPKLK
KEGSFILESM DLEHTQDQLP ILMIYNTILE VSFSVTTVSL QVVEDDKPFI RMILSDISAQ
VLKRTFDTSI KLSLAKLSIE DLYQDFKNES FKYLANSSID NNQQPLQSEL ENSSGIEREE
HLISISISSV QRNSPQYSGI SQQIDVSFNT LFVNINKKLV IKIMNLVDFV EKTIVETVMK
RREKRKRLEK DKDNQPLPTS ESNQQSIPQK QQQQQQQQQQ QQQQQQQQQQ QPPPPLQKQK
SQSHINLGKA FENSTFKIDK DNSIVIITTK MKSLVVNLNE DDQSYFALTV SDLSINFDID
LESSMMRFKL GNLTISDISK EASKDLNPLF GTDGEHIIEF YVYTLKNSKP LETITTTATA
KITLDNETKE TISTTLFEKV KEKPPSSPLK DSTSILQDEN EIFDTKIGVR MSSVKLIVVR
SLIDRIIDYF EEFAPNQIDQ ILADSAKGAV EIMSTRRINQ QKRILFEVDV NTPRIIFPKN
HYSPYVIIAD LGHISVFNKY KSNLTNSNGG DNTILSSEQV FVYASSVNFH ACKFQNGKVL
ESTIEPISNK INVHVNIESF LNPSLLEEDQ LDEPKQKVDG EISPINLKIS DQQYSLTIAI
IKSLIEPTIK QKKRKKRNHR RIESQLNLDN LYQNFLNVAS SNQDFKSVDV PIGLPLPTPI
LESLHDHKSS SSSSSSSPPP PPPPSSSQQQ QQQIIKSLDS IKDQITFHLP HISLEFFRKN
KESVVSFEID EFECSLIEYD NGNEYSNFSL GSIDLTDTRK STVNHFRRII HHSKEKNQQS
ADGSGLSPPL SKDEKEKKEM KKELQVYVFE KRGGDTQVKL ILDHFRGVIV PESLISMIDF
IMPGIKALSS LSEISPVSSP VLNVDTMNNN PHLFGRDPLK STVKAMLEEK NKKRMTTNTV
VHIETSNIDL SFVDNPATRE SRLLIARGSL FGKVTIQKSD SLTYKDIQLC IDEFILFKCR
ADNFDQTIES IIDPLSITTN ISMVEGKDYL PIDILIAVDP MHISISFQDL LLFLSLSKNI
ESLINSLNNL QQQHQQQNNN SNNNNNNNNN NNNNNNNNNN NNNNNNNDDK SKQEQQILRV
NFQSPSVELT VINDYGNKYC GIIDLKIRDI SVEVAQNRVL FAMFIAANYF NVSKSVWEPM
IEEWGCKFGI ETSEIGTKDQ SKNIVISSSG KLNLNITKSL FDTFTTIYPL IEIAMDISKA
DSTQSAYDLG YKTGVNNNLL QHYKSQNYHP FLLKNQTGLE IQYQISIICA SSFGDTQSND
FVGLSTSTSP STSTTTTSTS TSTSTSTSTS TSTSTSTSTS TSTSNPTTSS KTQTTKNKIL
EDGGEIYLDL KETLYSNGNT NQEKDKFRSI NNAQKYRDIT SISLFRINIV VDGFEAISNL
PIHKTGIYIC SLIPKVNVSS STKSLPSQQL VFEVRVSDFT KELIVRSNVL IVNETDHTLE
YLDKPTHTLR KVCPFILEPK QSKSLSICGG NSSSSSSSSS SSTTDMIFSF RPMIHQSFSS
SANLGNNQQR WTHPLNCNNG LLHPGTLLFD CPPNKFYCLQ IDQHLSNFQL EDQQINPLTG
GTIPQHQPSY HHISNQQYYE NSMVNDENQD NQSSSSMNRI IQKTNHIIKI VPPLVIYNQF
IIPIDITLHD QESNRTVGKE SIQENQTKGI YCVNVERLIM IKVHMKGFGS SGFIQINQSS
QNYINKQPRK LISTTSDQTF FVNIFQNELF GSIHVCLYPT FSIINQIGLN LSFKLNNLSD
NNNNNNNNNN NNNNTIHDQP VIIASVFNSQ TFSNLLTKNT PKNKTTTTTT TTAATTKPIQ
GTIDYKKDPK KWYNDKNISQ ISMFSPIPSN VGTSNNTSYL VLQVDGNSQW SQPISIQPNK
QEPITIIKEQ KESPTSSKIT KLSLQFLIST SIANESLTTN ITVDPQFILV NNLAQPLYYC
QKDSNLYKNY CLNPNETIPF HFFDDQLLKK IAFRLSPNND WSGCFNINKS ISFNIKLDKP
IDDDQDSDSD SGSSNSSSPS ISSSSTSPLN LSSSSAFSNC SSNNNNNNGG GGGSSDDQFY
LPMIQMVQEK GIYFVVVNPE TKDYPPYRVE NLISVPIAIH QVDTNEQPIP IFSNQSKRYV
WSNPMGTKRL GITIPNTNYK KSVSLDKLQY HQPYQVKHSN KTLDIKLDII ANGPTNILRL
TDLNDHNSIS KTLNNNNNNN NNNNNNNLNS VTKDNNNNNN QNNNNQNNNN QNNNNLNFEI
CIGGISVSLI DGHPKELMYI TLRGLQLRFK QYSTEQNINL SIHSFKVDNQ LYHTPYPVMI
QSYNSNNLKP TKHSFNVSLI RSGQYQPKEL HQVGIEYIKE LNFDIQPFDV EIDETILFHL
YNFIKSIKLP NNQNNNNNQN NNNQNNDFNN IDEYDEKEKN DGKIIKSIKL PYSNLPIPHS
IDSKRLYIEV LNLPLVDVTI SYCLSPHSRP LIGPWASVFS TIANIDKASL HLGSWKLEHQ
FVTANALALS LFSHYKWEVS KILLYSDVFG APLSLVENIT RGINDFIYES KEGLNNPENF
GKGLVKGTKS LVSNSIYGIF NSASKLTNTV GSAIAPFSMD ENYLAQRDRH TQPKHAIDGV
SNGIGRFTQG LAHGLSGLFD QPVKEFQDQG FSGLVKGFGK GILGTVVKPT VGAIDMINAA
TQGIRNSYLD PASSKSRSRP PRFIGPDAII KPFSFEKSFL QQVIKTTNTH QSLLSSKEWY
IIHFKVYDEK KTPYYLVIST QKFYLFDFKF NFILSIKLWD LDLSLKPKDD RYLSIQEIKK
DDESPRNPTY VLLLDQDGKS ISYSLLNSMH NIISTIKLDQ TYFVSN
//
