ID VSR4_ARATH Reviewed; 628 AA.
AC Q56ZQ3; O80979;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 19-JUL-2005, sequence version 2.
DT 27-MAR-2024, entry version 128.
DE RecName: Full=Vacuolar-sorting receptor 4;
DE Short=AtVSR4;
DE AltName: Full=BP80-like protein a;
DE Short=AtBP80a;
DE AltName: Full=Epidermal growth factor receptor-like protein 2b;
DE Short=AtELP2b;
DE Flags: Precursor;
GN Name=VSR4; Synonyms=BP80A, ELP2B; OrderedLocusNames=At2g14720;
GN ORFNames=F26C24.14;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10617197; DOI=10.1038/45471;
RA Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D.,
RA Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V.,
RA Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S.,
RA Cronin L.A., Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J.,
RA Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M.,
RA Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O.,
RA Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.;
RT "Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana.";
RL Nature 402:761-768(1999).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 549-628.
RC STRAIN=cv. Columbia;
RA Totoki Y., Seki M., Ishida J., Nakajima M., Enju A., Kamiya A.,
RA Narusaka M., Shin-i T., Nakagawa M., Sakamoto N., Oishi K., Kohara Y.,
RA Kobayashi M., Toyoda A., Sakaki Y., Sakurai T., Iida K., Akiyama K.,
RA Satou M., Toyoda T., Konagaya A., Carninci P., Kawai J., Hayashizaki Y.,
RA Shinozaki K.;
RT "Large-scale analysis of RIKEN Arabidopsis full-length (RAFL) cDNAs.";
RL Submitted (MAR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP TISSUE SPECIFICITY.
RX PubMed=10561538; DOI=10.1016/s0005-2728(99)00087-0;
RA Laval V., Chabannes M., Carriere M., Canut H., Barre A., Rouge P.,
RA Pont-Lezica R., Galaud J.-P.;
RT "A family of Arabidopsis plasma membrane receptors presenting animal beta-
RT integrin domains.";
RL Biochim. Biophys. Acta 1435:61-70(1999).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=11079568;
RX DOI=10.1002/1522-2683(20001001)21:16<3488::aid-elps3488>3.0.co;2-3;
RA Prime T.A., Sherrier D.J., Mahon P., Packman L.C., Dupree P.;
RT "A proteomic analysis of organelles from Arabidopsis thaliana.";
RL Electrophoresis 21:3488-3499(2000).
RN [7]
RP TISSUE SPECIFICITY.
RX PubMed=12493849; DOI=10.1093/jxb/erg018;
RA Laval V., Masclaux F., Serin A., Carriere M., Roldan C., Devic M.,
RA Pont-Lezica R.F., Galaud J.-P.;
RT "Seed germination is blocked in Arabidopsis putative vacuolar sorting
RT receptor (atbp80) antisense transformants.";
RL J. Exp. Bot. 54:213-221(2003).
RN [8]
RP GENE FAMILY, AND NOMENCLATURE.
RX PubMed=14657332; DOI=10.1073/pnas.2530568100;
RA Shimada T., Fuji K., Tamura K., Kondo M., Nishimura M., Hara-Nishimura I.;
RT "Vacuolar sorting receptor for seed storage proteins in Arabidopsis
RT thaliana.";
RL Proc. Natl. Acad. Sci. U.S.A. 100:16095-16100(2003).
CC -!- FUNCTION: Vacuolar-sorting receptor (VSR) involved in clathrin-coated
CC vesicles sorting from Golgi apparatus to vacuoles. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Single-pass type I
CC membrane protein {ECO:0000250}. Golgi apparatus membrane {ECO:0000250};
CC Single-pass type I membrane protein {ECO:0000250}. Cytoplasmic vesicle,
CC clathrin-coated vesicle membrane {ECO:0000250}; Single-pass type I
CC membrane protein {ECO:0000250}. Prevacuolar compartment membrane
CC {ECO:0000250}; Single-pass type I membrane protein {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed at low levels in seeds, seedlings, roots,
CC stems, leaves, flowers and siliques. {ECO:0000269|PubMed:10561538,
CC ECO:0000269|PubMed:12493849}.
CC -!- DOMAIN: The tyrosine-based internalization signal may be involved in
CC trafficking at the TGN. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the VSR (BP-80) family. {ECO:0000305}.
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DR EMBL; AC004705; AAC24185.1; -; Genomic_DNA.
DR EMBL; AC005398; AAM15052.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06326.1; -; Genomic_DNA.
DR EMBL; CP002685; AEC06327.1; -; Genomic_DNA.
DR EMBL; AY062744; AAL32822.1; -; mRNA.
DR EMBL; BT008390; AAP37749.1; -; mRNA.
DR EMBL; AK220910; BAD94353.1; -; mRNA.
DR PIR; T02604; T02604.
DR RefSeq; NP_179079.1; NM_127036.5.
DR RefSeq; NP_849954.1; NM_179623.2.
DR AlphaFoldDB; Q56ZQ3; -.
DR SMR; Q56ZQ3; -.
DR STRING; 3702.Q56ZQ3; -.
DR GlyCosmos; Q56ZQ3; 3 sites, No reported glycans.
DR PaxDb; 3702-AT2G14720-2; -.
DR ProteomicsDB; 242742; -.
DR EnsemblPlants; AT2G14720.1; AT2G14720.1; AT2G14720.
DR EnsemblPlants; AT2G14720.2; AT2G14720.2; AT2G14720.
DR GeneID; 815960; -.
DR Gramene; AT2G14720.1; AT2G14720.1; AT2G14720.
DR Gramene; AT2G14720.2; AT2G14720.2; AT2G14720.
DR KEGG; ath:AT2G14720; -.
DR Araport; AT2G14720; -.
DR TAIR; AT2G14720; VSR4.
DR eggNOG; ENOG502QSX2; Eukaryota.
DR HOGENOM; CLU_031082_1_0_1; -.
DR InParanoid; Q56ZQ3; -.
DR OMA; GINSCDD; -.
DR OrthoDB; 305396at2759; -.
DR PhylomeDB; Q56ZQ3; -.
DR PRO; PR:Q56ZQ3; -.
DR Proteomes; UP000006548; Chromosome 2.
DR ExpressionAtlas; Q56ZQ3; baseline and differential.
DR Genevisible; Q56ZQ3; AT.
DR GO; GO:0030665; C:clathrin-coated vesicle membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005768; C:endosome; HDA:TAIR.
DR GO; GO:0005794; C:Golgi apparatus; HDA:TAIR.
DR GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0005739; C:mitochondrion; HDA:TAIR.
DR GO; GO:0000325; C:plant-type vacuole; HDA:TAIR.
DR GO; GO:0005802; C:trans-Golgi network; HDA:TAIR.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0006623; P:protein targeting to vacuole; IGI:TAIR.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR CDD; cd00054; EGF_CA; 1.
DR CDD; cd02125; PA_VSR; 1.
DR Gene3D; 3.50.30.30; -; 1.
DR Gene3D; 2.10.25.10; Laminin; 3.
DR InterPro; IPR026823; cEGF.
DR InterPro; IPR001881; EGF-like_Ca-bd_dom.
DR InterPro; IPR000742; EGF-like_dom.
DR InterPro; IPR018097; EGF_Ca-bd_CS.
DR InterPro; IPR046450; PA_dom_sf.
DR InterPro; IPR003137; PA_domain.
DR PANTHER; PTHR22702; PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN; 1.
DR PANTHER; PTHR22702:SF1; PROTEASE-ASSOCIATED DOMAIN-CONTAINING PROTEIN 1; 1.
DR Pfam; PF12662; cEGF; 1.
DR Pfam; PF02225; PA; 1.
DR SMART; SM00179; EGF_CA; 1.
DR SUPFAM; SSF52025; PA domain; 1.
DR PROSITE; PS00010; ASX_HYDROXYL; 1.
DR PROSITE; PS01186; EGF_2; 1.
DR PROSITE; PS01187; EGF_CA; 1.
PE 2: Evidence at transcript level;
KW Calcium; Cytoplasmic vesicle; Disulfide bond; EGF-like domain;
KW Glycoprotein; Golgi apparatus; Membrane; Protein transport;
KW Reference proteome; Repeat; Signal; Transmembrane; Transmembrane helix;
KW Transport.
FT SIGNAL 1..24
FT /evidence="ECO:0000255"
FT CHAIN 25..628
FT /note="Vacuolar-sorting receptor 4"
FT /id="PRO_0000036466"
FT TOPO_DOM 25..569
FT /note="Lumenal"
FT /evidence="ECO:0000255"
FT TRANSMEM 570..590
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 591..628
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 56..168
FT /note="PA"
FT DOMAIN 416..466
FT /note="EGF-like 1"
FT DOMAIN 469..516
FT /note="EGF-like 2"
FT DOMAIN 517..559
FT /note="EGF-like 3; calcium-binding"
FT /evidence="ECO:0000255"
FT MOTIF 610..613
FT /note="Tyrosine-based internalization motif"
FT /evidence="ECO:0000250"
FT CARBOHYD 148
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 294
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 434
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 420..438
FT /evidence="ECO:0000250"
FT DISULFID 427..447
FT /evidence="ECO:0000250"
FT DISULFID 449..465
FT /evidence="ECO:0000250"
FT DISULFID 473..493
FT /evidence="ECO:0000250"
FT DISULFID 480..501
FT /evidence="ECO:0000250"
FT DISULFID 503..515
FT /evidence="ECO:0000250"
FT DISULFID 545..558
FT /evidence="ECO:0000250"
SQ SEQUENCE 628 AA; 69812 MW; E8FD3D33194DCF0E CRC64;
MKQLLCYLPW LLLLSLVVSP FNEARFVVEK NSLSVTSPES IKGTHDSAIG NFGIPQYGGS
MAGTVVYPKE NQKSCKEFSD FSISFKSQPG ALPTFLLVDR GDCFFALKVW NAQKAGASAV
LVADNVDEPL ITMDTPEEDV SSAKYIENIT IPSALVTKGF GEKLKKAISG GDMVNLNLDW
REAVPHPDDR VEYELWTNSN DECGVKCDML MEFVKDFKGA AQILEKGGFT QFRPHYITWY
CPHAFTLSRQ CKSQCINKGR YCAPDPEQDF SSGYDGKDVV VENLRQLCVY KVANETGKPW
VWWDYVTDFQ IRCPMKEKKY NKDCAESVIK SLGIDSRKID KCMGDPDADL DNPVLKEEQD
AQVGKGTRGD VTILPTLVVN NRQYRGKLEK SAVLKALCSG FEESTEPAIC LSTDMETNEC
LDNNGGCWQD KSANITACKD TFRGKVCVCP IVDGVRFKGD GYSHCEPSGP GRCTINNGGC
WHEERDGHAF SACVDKDSVK CECPPGFKGD GVKKCEDINE CKEKKACQCP ECSCKNTWGS
YECSCSGDLL YMRDHDTCIS KTGSQVKSAW AAVWLIMLSL GLAAAGAYLV YKYRLRQYMD
SEIRAIMAQY MPLDSQPEVP NHTNDERA
//
