ID W0AJG2_9SPHN Unreviewed; 531 AA.
AC W0AJG2;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 24-JAN-2024, entry version 38.
DE RecName: Full=Isocitrate lyase {ECO:0000256|ARBA:ARBA00017446};
DE AltName: Full=Isocitrase {ECO:0000256|ARBA:ARBA00031022};
DE AltName: Full=Isocitratase {ECO:0000256|ARBA:ARBA00031921};
GN ORFNames=NX02_24125 {ECO:0000313|EMBL:AHE56433.1};
OS Sphingomonas sanxanigenens DSM 19645 = NX02.
OC Bacteria; Pseudomonadota; Alphaproteobacteria; Sphingomonadales;
OC Sphingomonadaceae; Sphingomonas.
OX NCBI_TaxID=1123269 {ECO:0000313|EMBL:AHE56433.1, ECO:0000313|Proteomes:UP000018851};
RN [1] {ECO:0000313|EMBL:AHE56433.1, ECO:0000313|Proteomes:UP000018851}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NX02 {ECO:0000313|EMBL:AHE56433.1,
RC ECO:0000313|Proteomes:UP000018851};
RA Ma T., Huang H., Wu M., Li X., Li G.;
RT "Completed genome of Sphingomonas sanxanigenens NX02.";
RL Submitted (JUL-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-threo-isocitrate = glyoxylate + succinate;
CC Xref=Rhea:RHEA:13245, ChEBI:CHEBI:15562, ChEBI:CHEBI:30031,
CC ChEBI:CHEBI:36655; EC=4.1.3.1;
CC Evidence={ECO:0000256|ARBA:ARBA00023531};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR001362-3};
CC Note=Can also use Mn(2+) ion. {ECO:0000256|PIRSR:PIRSR001362-3};
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DR EMBL; CP006644; AHE56433.1; -; Genomic_DNA.
DR RefSeq; WP_025294545.1; NZ_CP006644.1.
DR AlphaFoldDB; W0AJG2; -.
DR STRING; 1123269.NX02_24125; -.
DR KEGG; ssan:NX02_24125; -.
DR PATRIC; fig|1123269.5.peg.4724; -.
DR eggNOG; COG2224; Bacteria.
DR HOGENOM; CLU_038243_0_0_5; -.
DR OrthoDB; 8629576at2; -.
DR Proteomes; UP000018851; Chromosome.
DR GO; GO:0004451; F:isocitrate lyase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR CDD; cd00377; ICL_PEPM; 1.
DR Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR InterPro; IPR039556; ICL/PEPM.
DR InterPro; IPR006254; Isocitrate_lyase.
DR InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR PANTHER; PTHR21631:SF3; BIFUNCTIONAL GLYOXYLATE CYCLE PROTEIN; 1.
DR PANTHER; PTHR21631; ISOCITRATE LYASE/MALATE SYNTHASE; 1.
DR Pfam; PF00463; ICL; 3.
DR PIRSF; PIRSF001362; Isocit_lyase; 3.
DR SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
PE 4: Predicted;
KW Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000313|EMBL:AHE56433.1};
KW Magnesium {ECO:0000256|PIRSR:PIRSR001362-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR001362-3};
KW Reference proteome {ECO:0000313|Proteomes:UP000018851}.
FT ACT_SITE 221
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-1"
FT BINDING 183
FT /ligand="Mg(2+)"
FT /ligand_id="ChEBI:CHEBI:18420"
FT /evidence="ECO:0000256|PIRSR:PIRSR001362-3"
SQ SEQUENCE 531 AA; 59213 MW; 95966BA63779F68A CRC64;
MTYQTHIAET GQLIQSHQGT WDGISAEAVA RMRLQNRFRT GLDIARYTAA IMRRDMAAYD
ADPAAYTQSL GCWHGFIGQQ KMISIKKHFG TTKRRYLYLS GWMIAALRSD FGPLPDQSMH
EKTSVPALIE ELYTFLRQAD ARELGMMFRE LDAARAAGNR IEEQRLLQAI DNYETHVVPI
IADIDAGFGN AEATYLLAKK MIEAGACALQ IENQVSDEKQ CGHQDGKVTV PHEDFLAKVR
ACRYAFLELG VEDGVIVTRT DSLGAGLTKQ IAFSKEPGDL GDQYNAFLDC AEVTDLSTVN
GDVIINRDGK LMRPKRLPSN LFQFREGTGE DRCVLDCITS LQNGADLLWI ETEKPHIEQI
ASMVDRIREV IPNAKLVYNN SPSFNWTLNF RQQVFDAWAA EGRDVSAYDR ARLMSVDYDG
TDLAAEADER IRTFQKDAAA RAGIFHHLIT LPTYHTAALS TDNLAREYFG EAGMLGYVLN
VQRQEIRQGI ACVKHQNMSG SDIGDDHKEY FAGEAALKAG GAHNTMNQFA A
//
