ID   W0HXI5_9GAMM            Unreviewed;       617 AA.
AC   W0HXI5;
DT   19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT   19-MAR-2014, sequence version 1.
DT   27-MAR-2024, entry version 38.
DE   SubName: Full=Protease 4 {ECO:0000313|EMBL:AHF76873.1};
GN   Name=sppA {ECO:0000313|EMBL:AHF76873.1};
GN   ORFNames=Sant_1820 {ECO:0000313|EMBL:AHF76873.1};
OS   Sodalis praecaptivus.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Bruguierivoracaceae; Sodalis.
OX   NCBI_TaxID=1239307 {ECO:0000313|EMBL:AHF76873.1, ECO:0000313|Proteomes:UP000019028};
RN   [1] {ECO:0000313|EMBL:AHF76873.1, ECO:0000313|Proteomes:UP000019028}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=HS1 {ECO:0000313|EMBL:AHF76873.1,
RC   ECO:0000313|Proteomes:UP000019028};
RX   PubMed=24407854; DOI=10.1093/gbe/evt210;
RA   Oakeson K.F., Gil R., Clayton A.L., Dunn D.M., von Niederhausern A.C.,
RA   Hamil C., Aoyagi A., Duval B., Baca A., Silva F.J., Vallier A.,
RA   Jackson D.G., Latorre A., Weiss R.B., Heddi A., Moya A., Dale C.;
RT   "Genome degeneration and adaptation in a nascent stage of symbiosis.";
RL   Genome Biol. Evol. 6:76-93(2014).
CC   -!- SIMILARITY: Belongs to the peptidase S49 family.
CC       {ECO:0000256|ARBA:ARBA00008683}.
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DR   EMBL; CP006569; AHF76873.1; -; Genomic_DNA.
DR   RefSeq; WP_025422000.1; NZ_CP006569.1.
DR   AlphaFoldDB; W0HXI5; -.
DR   KEGG; sod:Sant_1820; -.
DR   PATRIC; fig|1239307.3.peg.1998; -.
DR   HOGENOM; CLU_008856_1_1_6; -.
DR   OrthoDB; 9764363at2; -.
DR   Proteomes; UP000019028; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:InterPro.
DR   GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0006465; P:signal peptide processing; IEA:InterPro.
DR   CDD; cd07019; S49_SppA_1; 1.
DR   CDD; cd07018; S49_SppA_67K_type; 1.
DR   Gene3D; 6.20.330.10; -; 1.
DR   InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR   InterPro; IPR004634; Pept_S49_pIV.
DR   InterPro; IPR004635; Pept_S49_SppA.
DR   InterPro; IPR002142; Peptidase_S49.
DR   InterPro; IPR033854; S49_SppA_1.
DR   InterPro; IPR047217; S49_SppA_67K_type_N.
DR   NCBIfam; TIGR00705; SppA_67K; 1.
DR   NCBIfam; TIGR00706; SppA_dom; 1.
DR   PANTHER; PTHR33209; PROTEASE 4; 1.
DR   PANTHER; PTHR33209:SF1; SERINE PROTEASE SPPA, CHLOROPLASTIC; 1.
DR   Pfam; PF01343; Peptidase_S49; 2.
DR   PIRSF; PIRSF001217; Protease_4_SppA; 1.
DR   SUPFAM; SSF52096; ClpP/crotonase; 2.
PE   3: Inferred from homology;
KW   Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW   Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW   Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000313|EMBL:AHF76873.1};
KW   Reference proteome {ECO:0000313|Proteomes:UP000019028};
KW   Serine protease {ECO:0000256|ARBA:ARBA00022825};
KW   Transmembrane {ECO:0000256|SAM:Phobius};
KW   Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT   TRANSMEM        21..43
FT                   /note="Helical"
FT                   /evidence="ECO:0000256|SAM:Phobius"
FT   DOMAIN          139..288
FT                   /note="Peptidase S49"
FT                   /evidence="ECO:0000259|Pfam:PF01343"
FT   DOMAIN          390..541
FT                   /note="Peptidase S49"
FT                   /evidence="ECO:0000259|Pfam:PF01343"
FT   ACT_SITE        207
FT                   /note="Proton donor/acceptor"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001217-1"
FT   ACT_SITE        407
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001217-1"
SQ   SEQUENCE   617 AA;  67194 MW;  C7B7FA68148590DF CRC64;
     MRTLWRIISG PFKWGWRLLN FIREFILNVF LVVLIIVVVG IYLQVRHTPP TPTKGALVMD
     LTGTVVDKPA MNNKVRQLGR ELLGASSNRL QENSLFDVVD TLRQAKGDAN ITGLVLSLKD
     FAGADQASLQ YIGKALREFR DSGKPIYAVG DSYSQAQYFL ASYANKIYLT PQGAVDLHGI
     ATNNLYYKTL LDKLKVNSHI FRVGTYKSAV EPFLRDNMSD DAREADSRWV GQLWQHYLTT
     AAANRQITPQ QLFPGAQAML DGLRAVQGNT AQFALNNKWV DEVASRSAID DALTKAFGWN
     KRDKAFNGIS IYDYQPATPS QQGGQIAVIF ANGAIIDGPE TPGSVGGDTT ADQIRDARLD
     PDIKAIVFRV NSPGGSVTAS EVIRSELAAA RAAGKPVVVS MGGVAASGGY WVSTPANYII
     ASASTLTGSI GIFGVINTVE SSLDSIGVHT DGVATSPLAD VSMTKTLPSE FSQMMQLNVE
     NGYHNFIDLV AQSRHKTSEE VDKIGQGHVW IGSDAAQNGL VDQLGDFDDA VTKAAELAQL
     KQYQLNWYLD EPGLLDLLFT QTSASVYAVL PSVIQSWLPA PVTQVVDRLK MDAGLGFNWN
     DPQNRYAFCL SCEQVVR
//