ID W0THB2_KLUMD Unreviewed; 540 AA.
AC W0THB2;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 38.
DE SubName: Full=Vacuolar calcium ion transporter {ECO:0000313|EMBL:BAO41499.1};
GN Name=VCX1 {ECO:0000313|EMBL:BAO41499.1};
GN ORFNames=KLMA_60208 {ECO:0000313|EMBL:BAO41499.1};
OS Kluyveromyces marxianus (strain DMKU3-1042 / BCC 29191 / NBRC 104275)
OS (Yeast) (Candida kefyr).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Kluyveromyces.
OX NCBI_TaxID=1003335 {ECO:0000313|EMBL:BAO41499.1, ECO:0000313|Proteomes:UP000065495};
RN [1] {ECO:0000313|EMBL:BAO41499.1, ECO:0000313|Proteomes:UP000065495}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DMKU3-1042 / BCC 29191 / NBRC 104275
RC {ECO:0000313|Proteomes:UP000065495};
RX PubMed=25834639; DOI=10.1186/s13068-015-0227-x;
RA Lertwattanasakul N., Kosaka T., Hosoyama A., Suzuki Y., Rodrussamee N.,
RA Matsutani M., Murata M., Fujimoto N., Suprayogi, Tsuchikane K., Limtong S.,
RA Fujita N., Yamada M.;
RT "Genetic basis of the highly efficient yeast Kluyveromyces marxianus:
RT complete genome sequence and transcriptome analyses.";
RL Biotechnol. Biofuels 8:47-47(2015).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|ARBA:ARBA00004141}; Multi-
CC pass membrane protein {ECO:0000256|ARBA:ARBA00004141}.
CC -!- SIMILARITY: Belongs to the Ca(2+):cation antiporter (CaCA) (TC 2.A.19)
CC family. {ECO:0000256|ARBA:ARBA00008170}.
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DR EMBL; AP012218; BAO41499.1; -; Genomic_DNA.
DR AlphaFoldDB; W0THB2; -.
DR VEuPathDB; FungiDB:KLMA_60208; -.
DR OrthoDB; 2032402at2759; -.
DR Proteomes; UP000065495; Chromosome 6.
DR GO; GO:0005774; C:vacuolar membrane; IEA:UniProt.
DR GO; GO:0015369; F:calcium:proton antiporter activity; IEA:UniProt.
DR Gene3D; 1.20.1420.30; NCX, central ion-binding region; 1.
DR InterPro; IPR004713; CaH_exchang.
DR InterPro; IPR004837; NaCa_Exmemb.
DR InterPro; IPR044880; NCX_ion-bd_dom_sf.
DR PANTHER; PTHR31503:SF20; CA(2+)_H(+) EXCHANGER, PUTATIVE (EUROFUNG)-RELATED; 1.
DR PANTHER; PTHR31503; VACUOLAR CALCIUM ION TRANSPORTER; 1.
DR Pfam; PF01699; Na_Ca_ex; 2.
PE 3: Inferred from homology;
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}.
FT TRANSMEM 240..266
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 278..302
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 314..332
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 425..449
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 455..475
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 495..514
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 520..538
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 183..332
FT /note="Sodium/calcium exchanger membrane region"
FT /evidence="ECO:0000259|Pfam:PF01699"
FT DOMAIN 392..538
FT /note="Sodium/calcium exchanger membrane region"
FT /evidence="ECO:0000259|Pfam:PF01699"
FT REGION 18..66
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 349..384
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 21..38
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 40..66
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 540 AA; 59446 MW; 24CB0275485AA42B CRC64;
MNEAEELKSL ASGLSEASII QRPHLEDDKE EIDPNDNGNG KHHITISPTN TTGNCVPSRR
SPMRTLTTPG KYQQELALPE RYLERKPTVE VEAKGFYYFV SFMKQLRITL FDGTLNKLDA
FYTNMWSWPV FKNMMTFYQE HKDTKGLGTC LQVLRCCVET RTLVFLFLFP AGIVCYKQDK
DVGSVICNLL ALMYFAKLLS NATEYASEHF GPVFGALLNA TFGNLVELVI SGTTVSKDDA
VLTITSLIGS VLSNNMLVCG TCFFFGGLDG MQMHAKNAIA NHMLFLSCIT LALSLTSVIA
VIDSGKSLKF KENTSQIGAT LIFVCYIVYL IASANQEFKT LSNEKSKEEE RLLADTESSA
SDSQLQSLHT AEPGEEEEEE ETKNLPPKLV CFITLAIATA GLGPTGNFFV ESLKRLTDDK
PISKVFIGLI VLPLAGSLPE HISSLIAAYH GGMDLSVSLA VGSSNQILGM VLPIIQFISS
RYPKTGFTLY FEPYVAGYLF VSTFVCCFTL IHGLLFSVNV LHGTLLLVTY GIIVYLTFAN
//
