ID W0V6K2_9BURK Unreviewed; 743 AA.
AC W0V6K2;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 24-JAN-2024, entry version 37.
DE RecName: Full=Isocitrate dehydrogenase [NADP] {ECO:0000256|PIRNR:PIRNR009407};
DE EC=1.1.1.42 {ECO:0000256|PIRNR:PIRNR009407};
DE AltName: Full=Oxalosuccinate decarboxylase {ECO:0000256|PIRNR:PIRNR009407};
GN ORFNames=GJA_2273 {ECO:0000313|EMBL:CDG82907.1};
OS Janthinobacterium agaricidamnosum NBRC 102515 = DSM 9628.
OC Bacteria; Pseudomonadota; Betaproteobacteria; Burkholderiales;
OC Oxalobacteraceae; Janthinobacterium.
OX NCBI_TaxID=1349767 {ECO:0000313|EMBL:CDG82907.1, ECO:0000313|Proteomes:UP000027604};
RN [1] {ECO:0000313|EMBL:CDG82907.1, ECO:0000313|Proteomes:UP000027604}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=NBRC 102515 / DSM 9628 {ECO:0000313|Proteomes:UP000027604};
RX PubMed=25883287; DOI=10.1128/genomeA.00277-15;
RA Graupner K., Lackner G., Hertweck C.;
RT "Genome Sequence of Mushroom Soft-Rot Pathogen Janthinobacterium
RT agaricidamnosum.";
RL Genome Announc. 3:e00277-e00277(2015).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=D-threo-isocitrate + NADP(+) = 2-oxoglutarate + CO2 + NADPH;
CC Xref=Rhea:RHEA:19629, ChEBI:CHEBI:15562, ChEBI:CHEBI:16526,
CC ChEBI:CHEBI:16810, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.1.1.42;
CC Evidence={ECO:0000256|PIRNR:PIRNR009407};
CC -!- COFACTOR:
CC Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC Evidence={ECO:0000256|PIRSR:PIRSR009407-3};
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|PIRSR:PIRSR009407-3};
CC Note=Binds 1 Mg(2+) or Mn(2+) ion per subunit.
CC {ECO:0000256|PIRSR:PIRSR009407-3};
CC -!- SIMILARITY: Belongs to the monomeric-type IDH family.
CC {ECO:0000256|PIRNR:PIRNR009407}.
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DR EMBL; HG322949; CDG82907.1; -; Genomic_DNA.
DR RefSeq; WP_038491877.1; NZ_HG322949.1.
DR AlphaFoldDB; W0V6K2; -.
DR STRING; 1349767.GJA_2273; -.
DR KEGG; jag:GJA_2273; -.
DR PATRIC; fig|1349767.4.peg.4021; -.
DR eggNOG; COG2838; Bacteria.
DR HOGENOM; CLU_025308_1_0_4; -.
DR OrthoDB; 9807643at2; -.
DR Proteomes; UP000027604; Chromosome I.
DR GO; GO:0004450; F:isocitrate dehydrogenase (NADP+) activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR InterPro; IPR004436; Isocitrate_DH_NADP_mono.
DR NCBIfam; TIGR00178; monomer_idh; 1.
DR PANTHER; PTHR36999; ISOCITRATE DEHYDROGENASE [NADP]; 1.
DR PANTHER; PTHR36999:SF1; ISOCITRATE DEHYDROGENASE [NADP]; 1.
DR Pfam; PF03971; IDH; 1.
DR PIRSF; PIRSF009407; IDH_monmr; 1.
DR SUPFAM; SSF53659; Isocitrate/Isopropylmalate dehydrogenase-like; 1.
PE 3: Inferred from homology;
KW Glyoxylate bypass {ECO:0000256|PIRNR:PIRNR009407};
KW Magnesium {ECO:0000256|PIRSR:PIRSR009407-3};
KW Metal-binding {ECO:0000256|PIRSR:PIRSR009407-3};
KW NADP {ECO:0000256|PIRNR:PIRNR009407};
KW Oxidoreductase {ECO:0000256|PIRNR:PIRNR009407,
KW ECO:0000313|EMBL:CDG82907.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000027604};
KW Tricarboxylic acid cycle {ECO:0000256|PIRNR:PIRNR009407}.
FT BINDING 134..141
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT BINDING 147
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT BINDING 352
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT BINDING 550
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-2"
FT BINDING 551
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT BINDING 555
FT /ligand="Mn(2+)"
FT /ligand_id="ChEBI:CHEBI:29035"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-3"
FT SITE 257
FT /note="Critical for catalysis"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-1"
FT SITE 422
FT /note="Critical for catalysis"
FT /evidence="ECO:0000256|PIRSR:PIRSR009407-1"
SQ SEQUENCE 743 AA; 81914 MW; F25E8CE234A9101C CRC64;
MATQKSKIIY TLTDEAPMLA TYSLLPIIKK FTAPAGVDVV ASDISVASRV LAEFPEFLTD
EQKVPNTLAE LGKLTQNADT NIIKLPNISA SVAQLMACVR ELQGRGYKLP DYPEDPKTDE
DKALKARYGK CIGSAVNPVL REGNSDRRAP KAVKEFARKH PHSMGEWSQA SRTHVSHMHH
GDFYHGEKSL TLDSARDVKM ELITASGKTI VLKPKVSLKA GEIIDSMFMS KKALLEFYEK
ELDDAYKTGV MFSLHVKATM MKVSHPIVFG HCVRIFYKDA FTKHAALFEQ LGVNVNNGMV
NLYDKIETLP ASQKDEILKD LHACHANRPE LAMVDSAKGI TNFHSPNDVI VDASMPAMIR
IGGKMWGADG RPKDAKAVMP ESTFARIYQE MINFCKWHGN FDPRTMGTVP NVGLMAQQAE
EYGSHDKTFE VAEAGVANIT DLATGEVLLS QNVEEGDIWR MCQVKDAPIR DWVKLAVTRA
RNSGMPAVFW LDPYRPHENE VIKKVQTYLK DHDTNGLDIQ IMSQVRAMRY TLERVARGLD
TISVTGNILR DYLTDLFPIM ELGTSAKMLS IVPLMAGGGM YETGAGGSAP KHVKQLVEEN
HLRWDSLGEF LALAVSLEDM GIKTGNAKAK LLARTLDEAT GKLLDNNRSP SSRTGELDNR
GSHFYLALYW AQALAAQSDD AELKAHFAPL AKVLAENEAK IVEELKAVQG NAMDIGGYYF
PDPAKADAVM RPSASLNAAL ATI
//
