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Database: UniProt
Entry: W1JB90_9GAMM
LinkDB: W1JB90_9GAMM
Original site: W1JB90_9GAMM 
ID   W1JB90_9GAMM            Unreviewed;       107 AA.
AC   W1JB90;
DT   19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT   19-MAR-2014, sequence version 1.
DT   24-JAN-2024, entry version 56.
DE   RecName: Full=Iron-binding protein IscA {ECO:0000256|ARBA:ARBA00014591, ECO:0000256|HAMAP-Rule:MF_01429};
DE   AltName: Full=Iron-sulfur cluster assembly protein {ECO:0000256|ARBA:ARBA00032050, ECO:0000256|HAMAP-Rule:MF_01429};
GN   Name=iscA {ECO:0000256|HAMAP-Rule:MF_01429,
GN   ECO:0000313|EMBL:CDL86780.1};
GN   ORFNames=XCR1_60027 {ECO:0000313|EMBL:CDL86780.1};
OS   Xenorhabdus cabanillasii JM26.
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales;
OC   Morganellaceae; Xenorhabdus.
OX   NCBI_TaxID=1427517 {ECO:0000313|EMBL:CDL86780.1, ECO:0000313|Proteomes:UP000019197};
RN   [1] {ECO:0000313|EMBL:CDL86780.1, ECO:0000313|Proteomes:UP000019197}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=JM26 {ECO:0000313|EMBL:CDL86780.1,
RC   ECO:0000313|Proteomes:UP000019197};
RA   Gualtieri M., Ogier J.C., Pages S., Givaudan A., Gaudriault S.;
RT   "Draft genome sequence and annotation of the entomopathogenic bacterium,
RT   Xenorhabdus cabanillasi strain JM26.";
RL   Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Is able to transfer iron-sulfur clusters to apo-ferredoxin.
CC       Multiple cycles of [2Fe2S] cluster formation and transfer are observed,
CC       suggesting that IscA acts catalytically. Recruits intracellular free
CC       iron so as to provide iron for the assembly of transient iron-sulfur
CC       cluster in IscU in the presence of IscS, L-cysteine and the thioredoxin
CC       reductase system TrxA/TrxB. {ECO:0000256|HAMAP-Rule:MF_01429}.
CC   -!- COFACTOR:
CC       Name=Fe cation; Xref=ChEBI:CHEBI:24875;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01429};
CC       Note=Binds 2 iron ions per dimer. The dimer may bind additional iron
CC       ions. {ECO:0000256|HAMAP-Rule:MF_01429};
CC   -!- SUBUNIT: Homodimer; may form tetramers and higher multimers.
CC       {ECO:0000256|HAMAP-Rule:MF_01429}.
CC   -!- SIMILARITY: Belongs to the HesB/IscA family.
CC       {ECO:0000256|ARBA:ARBA00006718, ECO:0000256|HAMAP-Rule:MF_01429}.
CC   -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC       whole genome shotgun (WGS) entry which is preliminary data.
CC       {ECO:0000313|EMBL:CDL86780.1}.
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DR   EMBL; CBXE010000453; CDL86780.1; -; Genomic_DNA.
DR   RefSeq; WP_038267861.1; NZ_NJGH01000006.1.
DR   AlphaFoldDB; W1JB90; -.
DR   OrthoDB; 9801228at2; -.
DR   Proteomes; UP000019197; Unassembled WGS sequence.
DR   GO; GO:0051537; F:2 iron, 2 sulfur cluster binding; IEA:UniProt.
DR   GO; GO:0005506; F:iron ion binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0016226; P:iron-sulfur cluster assembly; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.60.300.12; HesB-like domain; 1.
DR   HAMAP; MF_01429; Fe_S_insert_IscA; 1.
DR   InterPro; IPR000361; FeS_biogenesis.
DR   InterPro; IPR016092; FeS_cluster_insertion.
DR   InterPro; IPR017870; FeS_cluster_insertion_CS.
DR   InterPro; IPR035903; HesB-like_dom_sf.
DR   InterPro; IPR011302; IscA_proteobacteria.
DR   NCBIfam; TIGR00049; iron-sulfur cluster assembly accessory protein; 1.
DR   NCBIfam; TIGR02011; IscA; 1.
DR   PANTHER; PTHR10072:SF41; IRON-SULFUR CLUSTER ASSEMBLY 1 HOMOLOG, MITOCHONDRIAL; 1.
DR   PANTHER; PTHR10072; IRON-SULFUR CLUSTER ASSEMBLY PROTEIN; 1.
DR   Pfam; PF01521; Fe-S_biosyn; 1.
DR   SUPFAM; SSF89360; HesB-like domain; 1.
DR   PROSITE; PS01152; HESB; 1.
PE   3: Inferred from homology;
KW   Iron {ECO:0000256|HAMAP-Rule:MF_01429};
KW   Metal-binding {ECO:0000256|HAMAP-Rule:MF_01429}.
FT   DOMAIN          1..103
FT                   /note="FeS cluster biogenesis"
FT                   /evidence="ECO:0000259|Pfam:PF01521"
FT   BINDING         35
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_01429"
FT   BINDING         99
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_01429"
FT   BINDING         101
FT                   /ligand="Fe cation"
FT                   /ligand_id="ChEBI:CHEBI:24875"
FT                   /evidence="ECO:0000256|HAMAP-Rule:MF_01429"
SQ   SEQUENCE   107 AA;  11520 MW;  1D9E9A2E2299411F CRC64;
     MSISLTESAA RRVLAFLDNR GKGVGLRLGV RTSGCSGMAY VLEFADAIDD EDQVFEDKGV
     KVIVDGKSIV YLDGTELDFV KEGLNEGFKF NNPNVSSECG CGESFNV
//
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