ID W1N701_9GAMM Unreviewed; 734 AA.
AC W1N701;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE RecName: Full=DNA 3'-5' helicase {ECO:0000256|ARBA:ARBA00034808};
DE EC=5.6.2.4 {ECO:0000256|ARBA:ARBA00034808};
DE AltName: Full=DNA 3'-5' helicase II {ECO:0000256|ARBA:ARBA00034923};
GN Name=uvrD {ECO:0000313|EMBL:ERL50951.1};
GN ORFNames=BJB45_20365 {ECO:0000313|EMBL:ERL50951.1};
OS Halomonas huangheensis.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Oceanospirillales;
OC Halomonadaceae; Halomonas.
OX NCBI_TaxID=1178482 {ECO:0000313|EMBL:ERL50951.1, ECO:0000313|Proteomes:UP000019113};
RN [1] {ECO:0000313|EMBL:ERL50951.1, ECO:0000313|Proteomes:UP000019113}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=BJGMM-B45 {ECO:0000313|EMBL:ERL50951.1,
RC ECO:0000313|Proteomes:UP000019113};
RA Miao C., Wan Y., Jin W.;
RT "draft genome of Halomonas huanghegensis, strain BJGMM-B45T.";
RL Submitted (AUG-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + H2O = ADP + H(+) + phosphate; Xref=Rhea:RHEA:13065,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:456216; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034618};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Couples ATP hydrolysis with the unwinding of duplex DNA by
CC translocating in the 3'-5' direction.; EC=5.6.2.4;
CC Evidence={ECO:0000256|ARBA:ARBA00034617};
CC -!- SIMILARITY: Belongs to the helicase family. UvrD subfamily.
CC {ECO:0000256|ARBA:ARBA00009922}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ERL50951.1}.
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DR EMBL; AVBC01000035; ERL50951.1; -; Genomic_DNA.
DR RefSeq; WP_021819479.1; NZ_CP013106.1.
DR AlphaFoldDB; W1N701; -.
DR STRING; 1178482.AR456_20520; -.
DR KEGG; hhu:AR456_20520; -.
DR PATRIC; fig|1178482.3.peg.2525; -.
DR eggNOG; COG0210; Bacteria.
DR OrthoDB; 9806690at2; -.
DR Proteomes; UP000019113; Unassembled WGS sequence.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:RHEA.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003678; F:DNA helicase activity; IEA:InterPro.
DR CDD; cd17932; DEXQc_UvrD; 1.
DR CDD; cd18807; SF1_C_UvrD; 1.
DR Gene3D; 1.10.10.160; -; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 2.
DR InterPro; IPR013986; DExx_box_DNA_helicase_dom_sf.
DR InterPro; IPR014017; DNA_helicase_UvrD-like_C.
DR InterPro; IPR000212; DNA_helicase_UvrD/REP.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR014016; UvrD-like_ATP-bd.
DR PANTHER; PTHR11070:SF2; ATP-DEPENDENT DNA HELICASE SRS2; 1.
DR PANTHER; PTHR11070; UVRD / RECB / PCRA DNA HELICASE FAMILY MEMBER; 1.
DR Pfam; PF21196; PcrA_UvrD_tudor; 1.
DR Pfam; PF00580; UvrD-helicase; 1.
DR Pfam; PF13361; UvrD_C; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR PROSITE; PS51198; UVRD_HELICASE_ATP_BIND; 1.
DR PROSITE; PS51217; UVRD_HELICASE_CTER; 1.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Helicase {ECO:0000256|ARBA:ARBA00022806, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU00560};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741, ECO:0000256|PROSITE-
KW ProRule:PRU00560}; Reference proteome {ECO:0000313|Proteomes:UP000019113}.
FT DOMAIN 9..287
FT /note="UvrD-like helicase ATP-binding"
FT /evidence="ECO:0000259|PROSITE:PS51198"
FT DOMAIN 288..571
FT /note="UvrD-like helicase C-terminal"
FT /evidence="ECO:0000259|PROSITE:PS51217"
FT BINDING 30..37
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00560"
SQ SEQUENCE 734 AA; 83289 MW; 699804F8EA1D7F3F CRC64;
MDDVTAIIDG LNPAQHEAVC APQGNMLVLA GAGSGKTRVL VHRIAWLMQV EGLSPYAVLA
VTFTNKAARE MRTRLESVLR MSLRHVWVGT FHSIAHRLLR THWQDARLPQ HFQIIDSDDQ
LRLIKRLLKD GRIDDERFPP RQVQSFISGC KEEGLRPHQV KVNGDAYVEQ MAELYNVYQL
TCERGGLVDF GELLLRSLEL LRDNPALLAH YQERFGHVLV DEFQDTNTLQ YAWLKLLTGQ
RTPMTVVGDD DQSIYGWRGA RIENIRRFEE EFPDTRTVRL EQNYRSTSAI LEAANTLIRQ
NSDRMGKELW TAGERGEPIS VYAGFNDMDE ARFIVDTIKS RVDEGINRRD IAILYRSNAQ
SRVLEETLIR QGMPYRIYGG HRFYERLEIK NSLAYLRLLL NRDDDASLER VINVPARGIG
TRTVEILRER ARHTEVPLWQ ALHDALADGS LKGRAATSLK AFADLIDGCD DEASGLELHR
IIELINKRTG LIEHHRNEKG EKGQARVENL EELINAARAF VQGETQGDIE VGEGMVALEA
FLAEAALNAG DHEADEFEDS VQLMTLHSAK GLEFPVVFIA GVEEGLFPHR MSLEEHGRLE
EERRLCYVGV TRAMHKLYLT HAEIRRMHGK ETFQRPSRFL RELPESLLEE VRLRGQVSRP
VSVRPSPSRW QQASIDASDD LPALALGQRV AHPVFGEGVI INAEGQGERA RVHVSFEDEG
DKWLVLGFAK LTPL
//
