ID W2K886_PHYPR Unreviewed; 2322 AA.
AC W2K886;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 48.
DE SubName: Full=Uncharacterized protein {ECO:0000313|EMBL:ETL81421.1};
GN ORFNames=L917_18206 {ECO:0000313|EMBL:ETL81421.1};
OS Phytophthora parasitica (Potato buckeye rot agent).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=4792 {ECO:0000313|EMBL:ETL81421.1};
RN [1] {ECO:0000313|EMBL:ETL81421.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CHvinca01 {ECO:0000313|EMBL:ETL81421.1};
RG The Broad Institute Genomics Platform;
RA Russ C., Tyler B., Panabieres F., Shan W., Tripathy S., Grunwald N.,
RA Machado M., Johnson C.S., Arredondo F., Hong C., Coffey M., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Abouelleil A., Alvarado L.,
RA Chapman S.B., Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Ireland A., Larimer J., McCowan C., Murphy C.,
RA Pearson M., Poon T.W., Priest M., Roberts A., Saif S., Shea T., Sykes S.,
RA Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Phytophthora parasitica CHvinca01.";
RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the peptidase C2 family.
CC {ECO:0000256|ARBA:ARBA00007623}.
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DR EMBL; KI682514; ETL81421.1; -; Genomic_DNA.
DR EnsemblProtists; ETL81421; ETL81421; L917_18206.
DR VEuPathDB; FungiDB:PPTG_05165; -.
DR Proteomes; UP000054423; Unassembled WGS sequence.
DR GO; GO:0005509; F:calcium ion binding; IEA:InterPro.
DR GO; GO:0004198; F:calcium-dependent cysteine-type endopeptidase activity; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd00044; CysPc; 1.
DR CDD; cd00051; EFh; 1.
DR Gene3D; 2.60.120.380; -; 1.
DR Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR Gene3D; 1.10.238.10; EF-hand; 2.
DR InterPro; IPR022684; Calpain_cysteine_protease.
DR InterPro; IPR036213; Calpain_III_sf.
DR InterPro; IPR011992; EF-hand-dom_pair.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR002048; EF_hand_dom.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR000169; Pept_cys_AS.
DR InterPro; IPR001300; Peptidase_C2_calpain_cat.
DR PANTHER; PTHR10183; CALPAIN; 1.
DR PANTHER; PTHR10183:SF379; CALPAIN-A-RELATED; 1.
DR Pfam; PF13202; EF-hand_5; 1.
DR Pfam; PF13499; EF-hand_7; 1.
DR Pfam; PF00648; Peptidase_C2; 1.
DR PRINTS; PR00704; CALPAIN.
DR SMART; SM00230; CysPc; 1.
DR SMART; SM00054; EFh; 3.
DR SUPFAM; SSF49758; Calpain large subunit, middle domain (domain III); 1.
DR SUPFAM; SSF54001; Cysteine proteinases; 1.
DR SUPFAM; SSF47473; EF-hand; 1.
DR PROSITE; PS50203; CALPAIN_CAT; 1.
DR PROSITE; PS00018; EF_HAND_1; 3.
DR PROSITE; PS50222; EF_HAND_2; 2.
DR PROSITE; PS00139; THIOL_PROTEASE_CYS; 1.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837};
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Hydrolase {ECO:0000256|PROSITE-ProRule:PRU00239};
KW Protease {ECO:0000256|PROSITE-ProRule:PRU00239};
KW Thiol protease {ECO:0000256|PROSITE-ProRule:PRU00239}.
FT DOMAIN 77..477
FT /note="Calpain catalytic"
FT /evidence="ECO:0000259|PROSITE:PS50203"
FT DOMAIN 1146..1181
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT DOMAIN 1303..1338
FT /note="EF-hand"
FT /evidence="ECO:0000259|PROSITE:PS50222"
FT COILED 2135..2223
FT /evidence="ECO:0000256|SAM:Coils"
FT ACT_SITE 183
FT /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT ECO:0000256|PROSITE-ProRule:PRU00239"
FT ACT_SITE 366
FT /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT ECO:0000256|PROSITE-ProRule:PRU00239"
FT ACT_SITE 391
FT /evidence="ECO:0000256|PIRSR:PIRSR622684-1,
FT ECO:0000256|PROSITE-ProRule:PRU00239"
SQ SEQUENCE 2322 AA; 258234 MW; EC67677D363D1C0F CRC64;
MRSAVKDNGA ADLHTLQEEL KKLGISVVSG RQDTVISVKI KGLAGRALIR GLKLADQLLQ
EKIKHAIKAC TDPATKFFTD PTFGPCSSDP DGAAAICKAG GVIPSKGGSQ HQAKVIGLLQ
RGKIRWERPI YAPDNEEDDI SPQEDEDDIY VMSSSDNVFA SNATLFADGV SSGDVIQGNL
GDCWFLSALS VVATRSDLLA QTFWRRDQHK SKGLFVCKFM KNFVWNYVLI DDRLPVFGFT
DKKPGKPYFA RCRNPNELWV SLIEKAYAKL HGSYEALIGG FVDCALNDLT GMCAEQVILR
EGFPGFGENP YTPAKPQQKH GDPFWEKITR YKNSGTLMGC SIQPPVTSTK EVAVESSAGN
GLYFKHAYAL VDAADIKTAK GEYVRLVKLR NPWGMGEWTG PWSDSSDERA ANEDAIDKFF
KIMKRKVGAN ADKRVFMTLN PQGRVGADEI SQEEVIEINA NDGTFFMSFD AWMQSFTHFF
AGIDFPDSWH GRRSEGSWNE ANCGGNTTKS TWINNPHFEL ILEQRARIFA SLSQEDPRGS
ENLKIVPVGF HICSLSPVGN DPNKFEIREP TKKLDAYYRS YKPVDRDNYM TGKRPEPLPP
AIIPGTVIPG IDDDGVPQAA YTFKQAVSVE ATMEPGRYCI IPSMYMRTDK VTGNTNVGNF
WISVYSDKPT FRLEGGEKIV EEEEGDEETQ TNGSQSYKNL NAGATTLTRQ FSSTNSSRKS
ETIVATGNAV AAEKRRNFEN EKEELLRQAK RYGIGLRELR TAFGKTPKVS KAECRQKLQK
LGFNVASWDD DKIGLLFGGL EEGPTSGLIQ TDRILNLFTP DIQEEKMACE LPDMEEDGDT
PDSIHREGIL EIELHGASGL AVGEKRQSLI QRKVPIPTPT FSTSQISLRR KIAAEILERD
PSMAWYLRQF AKKSTLLANT HNTSKQNRDG SVRSQLHIPT PVAAFYELLE KEYAARIISR
CDVEDELEYV VPVSRTPSYS KRDGISPFYP SLSSKSAPEW NLPPTPLNEL ELHKSSRIRD
LEAKRSTKLA LLLQHKQQAL GDAEKTASLP RLIGGRKSGS SHVKCIDCGA RVLVGGRSKT
ATSQDIDSCP CNGANCVNVF CSPCYSLLPT KSKLCEECYQ HEISPEERFG EQLRGVLIEK
IGASDQQRSH LEEVFRSFDV DGSGSLSPQE FEKMLQLLNI QPALTQRQKA FLVGQFDANG
DGEISLIEFK HWILRDHVWV ESVAPTSHKT IPHTAEVMST VIAPLCEEII DLAYNAYAFS
STIGSWSRSS SSTTECQKKT LQSGETGVII FQRVIKLSVT SGGEANVAEK VFEQVDDDGS
GSIDQFEFVA LLSALGIHLN SEDAVLLMNR FGAASTGSGR ITIEKAAFIE YVGQMSTKTF
SRVGDGSLDK ILVKLAEFAA TNPVARESLQ DELQQLHENI TQKELLRICK NLDEKASLIG
GVDELRSLAM NLAFDHDMNA TKTEREGADQ HGSLKSSGDI LARMLLLESG DLMKLVRTFD
VTSTCRELAS HLEIRSGTNS TRTIWKSVFG ADTTDVVHLR DFISGVLKTG FLIEVQNEPD
KLGVATHDRL ITPLLLSLAV DAVRTDCHVL STSPEASPVI SFNLFRLIVR LQKIKEIELK
FDHALTNFMQ LCQGEQQYLV TIALDASRNL VVRAGDPVFK FSADFELRED EFDYQNLIAQ
LGSASFERRQ QETHEVSREM AFAKAPYGLI TSEFQPELNN SMLTIIERLR AELRCRHHST
TSHTSRLHIL SLTMVESEAF VSTLRNLLSL VELPFFWSVS PRSLVFSIDS EYLDQQSKPS
FQQLVADTLS LHGSQLPPPL RALATFVAHT ASSLLVRYEV VGKYTNVETS WQEFQDFISG
HQNAYAVMEL HPQGSVLTTR VARASGSGAV QWNFKTQIKM DEPKLCDHRI DRPVVFTDTV
KVSCIPGSTE NINLIRGDSE GPGHFVVISV RRALPNGSDF PKPRLYCTAY DPLTSCDYAV
EGYPPDWSVD FFDVTVNPSF ESQWQTMLDS MRLGVTITPK LAITVYNKQP KTDKLVGECE
VSIGSAVVQE GHIFEERVAL RPPVMNPTSA CAGVTSLTFR FDVEKTSSIR EDVVAERRQQ
DTGTRVPIKL GAQYLAGEIV TPRTESVPDN GNDRVKELQT SMTAMEMSKL EAQEQVKQLK
AQIQQLSYAS NKTTEDNAER WKRRLEQARQ EQFAAEEQHA ARLAALQEEI RHLSEETQKQ
KNAHREASNL KDCRLSADAS AYDIFSSIRE ILTRRCPERP YNGLKKALAA EAEVPGKVTF
AVLNDVLDDF GLALSSDQRS TLANLFDPEM VGRVNIEGEF VW
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