ID W2Q556_PHYPN Unreviewed; 937 AA.
AC W2Q556;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 24-JAN-2024, entry version 33.
DE RecName: Full=Beta-mannosidase B {ECO:0000256|ARBA:ARBA00041069};
DE EC=3.2.1.25 {ECO:0000256|ARBA:ARBA00012754};
DE AltName: Full=Mannanase B {ECO:0000256|ARBA:ARBA00041614};
GN ORFNames=PPTG_12094 {ECO:0000313|EMBL:ETN08267.1};
OS Phytophthora parasitica (strain INRA-310).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=761204 {ECO:0000313|EMBL:ETN08267.1, ECO:0000313|Proteomes:UP000018817};
RN [1] {ECO:0000313|Proteomes:UP000018817}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=INRA-310 {ECO:0000313|Proteomes:UP000018817};
RG The Broad Institute Genome Sequencing Platform;
RA Russ C., Tyler B., Panabieres F., Shan W., Tripathy S., Grunwald N.,
RA Machado M., Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B.,
RA Abouelleil A., Alvarado L., Arachchi H.M., Berlin A., Chapman S.B.,
RA Gearin G., Goldberg J., Griggs A., Gujja S., Hansen M., Heiman D.,
RA Howarth C., Larimer J., Lui A., MacDonald P.J.P., McCowen C.,
RA Montmayeur A., Murphy C., Neiman D., Pearson M., Priest M., Roberts A.,
RA Saif S., Shea T., Sisk P., Stolte C., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RL Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ETN08267.1, ECO:0000313|Proteomes:UP000018817}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=INRA-310 {ECO:0000313|EMBL:ETN08267.1,
RC ECO:0000313|Proteomes:UP000018817};
RG The Broad Institute Genomics Platform;
RA Russ C., Tyler B., Panabieres F., Shan W., Tripathy S., Grunwald N.,
RA Machado M., Johnson C.S., Arredondo F., Hong C., Coffey M., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Abouelleil A., Alvarado L.,
RA Chapman S.B., Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Ireland A., Larimer J., McCowan C., Murphy C.,
RA Pearson M., Poon T.W., Priest M., Roberts A., Saif S., Shea T., Sykes S.,
RA Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Phytophthora parasitica INRA-310.";
RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing beta-D-mannose residues
CC in beta-D-mannosides.; EC=3.2.1.25;
CC Evidence={ECO:0000256|ARBA:ARBA00000829};
CC -!- PATHWAY: Glycan metabolism; N-glycan degradation.
CC {ECO:0000256|ARBA:ARBA00004740}.
CC -!- SUBUNIT: Homodimer. {ECO:0000256|ARBA:ARBA00011738}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000256|ARBA:ARBA00004613}.
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 2 family. Beta-
CC mannosidase B subfamily. {ECO:0000256|ARBA:ARBA00038429}.
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DR EMBL; KI669589; ETN08267.1; -; Genomic_DNA.
DR RefSeq; XP_008906555.1; XM_008908307.1.
DR AlphaFoldDB; W2Q556; -.
DR EnsemblProtists; ETN08267; ETN08267; PPTG_12094.
DR GeneID; 20181590; -.
DR VEuPathDB; FungiDB:PPTG_12094; -.
DR OrthoDB; 2504097at2759; -.
DR Proteomes; UP000018817; Unassembled WGS sequence.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0004567; F:beta-mannosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 2.
DR InterPro; IPR036156; Beta-gal/glucu_dom_sf.
DR InterPro; IPR041625; Beta-mannosidase_Ig.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR006102; Glyco_hydro_2_Ig-like.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR041447; Mannosidase_ig.
DR PANTHER; PTHR43730; BETA-MANNOSIDASE; 1.
DR PANTHER; PTHR43730:SF1; BETA-MANNOSIDASE; 1.
DR Pfam; PF00703; Glyco_hydro_2; 1.
DR Pfam; PF17753; Ig_mannosidase; 1.
DR Pfam; PF17786; Mannosidase_ig; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF49303; beta-Galactosidase/glucuronidase domain; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000018817};
KW Secreted {ECO:0000256|ARBA:ARBA00022525}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..20
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 21..937
FT /note="Beta-mannosidase B"
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5004822183"
FT DOMAIN 228..324
FT /note="Glycoside hydrolase family 2 immunoglobulin-like
FT beta-sandwich"
FT /evidence="ECO:0000259|Pfam:PF00703"
FT DOMAIN 731..832
FT /note="Mannosidase Ig/CBM-like"
FT /evidence="ECO:0000259|Pfam:PF17786"
FT DOMAIN 852..905
FT /note="Beta-mannosidase Ig-fold"
FT /evidence="ECO:0000259|Pfam:PF17753"
SQ SEQUENCE 937 AA; 106242 MW; 210BF445A4E61496 CRC64;
MRGLQLTFTA LLATTTSLNA AIVPLDRWTF RSANGSVRVD NASVPGTSHV HLMEAGVIGD
PYYRFNEREY QWVVQETWTY ETQVTLPSDV SSKPTLVFET LDGVAHVTVN GKQVATTANS
FVSHRIDVAA VLLPGVNHIQ VEFQPVLQYT RNQADTYPYF VPATENFNTW TEPTRRSFAR
KAGSDFGWDW GPAFVTSGIA GAAYIEFGEP VVKLKDLQVV QVFPGGKEDL SVVDVTVRVA
LDGGKAKHEN VTFDLFVNEE KKTTILTTIP EDWKEDTAIE LLFRLENPKL WWSVGYGEPY
LYGIRVDAWN SDFNSTLSHK IGIRQVELVQ DDTHAGNVAG KTFYFKINQV PIFIKGANWI
PADSFPTRTK ASTISHLLES VRAANMNMVR VWGGGRYESD LFYAECDRLG ILVWQELMFA
CGMYPRDTAF LQNVLKEVSV QVSRLRKYTS IAIWGGNNEN ENMMEQFVEA PIFPPGTTFN
RDIAVVDFTT LFVDLIRPII VALDSSRPFV DTSPSNGLYS VDPYVKRWGP TNGVAFGDVH
FYDYNNDCQD YRMYPRARFI SEFGFQSWPS ADSLHNISSK DDWDSFQAFW KFLKYRERHE
NGTTQMVTQL ERRFHVPFPF RNEKWLGEVS DVQDDKSFGF SISKRIESYL YLTQIQQSLC
YQTAIQTWRR GKNVELGMTM GILYWQLNDI WQGSSWSSIE YSGRWKSLHY VAKREFASFI
ISIHKREDSK AVEVYAVSDV NEDLHVDVVY EIRRTKCGSL VKSIDRQAIT VAALESRLIE
ELDIEALLDH DATSCHHRSC FLHVHCVVKS STTGLETAAC EDTNHFFAPY KHLNLGWGEV
AAHSVVKTES GNAVVFSVEL VATDFVALFV EVDVPGVLGY WSSNSFLMVG NETKMLNFTV
SASENVDMIT VDAFSRLITV NWLQKSYDNS VEDVAVQ
//
