ID W2RG25_PHYPN Unreviewed; 686 AA.
AC W2RG25;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 36.
DE RecName: Full=HECT-type E3 ubiquitin transferase {ECO:0000256|ARBA:ARBA00012485};
DE EC=2.3.2.26 {ECO:0000256|ARBA:ARBA00012485};
GN ORFNames=PPTG_00734 {ECO:0000313|EMBL:ETN24373.1};
OS Phytophthora parasitica (strain INRA-310).
OC Eukaryota; Sar; Stramenopiles; Oomycota; Peronosporales; Peronosporaceae;
OC Phytophthora.
OX NCBI_TaxID=761204 {ECO:0000313|EMBL:ETN24373.1, ECO:0000313|Proteomes:UP000018817};
RN [1] {ECO:0000313|Proteomes:UP000018817}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=INRA-310 {ECO:0000313|Proteomes:UP000018817};
RG The Broad Institute Genome Sequencing Platform;
RA Russ C., Tyler B., Panabieres F., Shan W., Tripathy S., Grunwald N.,
RA Machado M., Young S.K., Zeng Q., Gargeya S., Fitzgerald M., Haas B.,
RA Abouelleil A., Alvarado L., Arachchi H.M., Berlin A., Chapman S.B.,
RA Gearin G., Goldberg J., Griggs A., Gujja S., Hansen M., Heiman D.,
RA Howarth C., Larimer J., Lui A., MacDonald P.J.P., McCowen C.,
RA Montmayeur A., Murphy C., Neiman D., Pearson M., Priest M., Roberts A.,
RA Saif S., Shea T., Sisk P., Stolte C., Sykes S., Wortman J., Nusbaum C.,
RA Birren B.;
RL Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ETN24373.1, ECO:0000313|Proteomes:UP000018817}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=INRA-310 {ECO:0000313|EMBL:ETN24373.1,
RC ECO:0000313|Proteomes:UP000018817};
RG The Broad Institute Genomics Platform;
RA Russ C., Tyler B., Panabieres F., Shan W., Tripathy S., Grunwald N.,
RA Machado M., Johnson C.S., Arredondo F., Hong C., Coffey M., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Abouelleil A., Alvarado L.,
RA Chapman S.B., Gainer-Dewar J., Goldberg J., Griggs A., Gujja S., Hansen M.,
RA Howarth C., Imamovic A., Ireland A., Larimer J., McCowan C., Murphy C.,
RA Pearson M., Poon T.W., Priest M., Roberts A., Saif S., Shea T., Sykes S.,
RA Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Phytophthora parasitica INRA-310.";
RL Submitted (NOV-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine +
CC [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-
CC cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.;
CC EC=2.3.2.26; Evidence={ECO:0000256|ARBA:ARBA00000885};
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DR EMBL; KI669561; ETN24373.1; -; Genomic_DNA.
DR RefSeq; XP_008890438.1; XM_008892190.1.
DR AlphaFoldDB; W2RG25; -.
DR STRING; 761204.W2RG25; -.
DR EnsemblProtists; ETN24373; ETN24373; PPTG_00734.
DR GeneID; 20171080; -.
DR VEuPathDB; FungiDB:PPTG_00734; -.
DR OMA; PCFTANS; -.
DR OrthoDB; 5478214at2759; -.
DR Proteomes; UP000018817; Unassembled WGS sequence.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd00078; HECTc; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR PANTHER; PTHR11254; HECT DOMAIN UBIQUITIN-PROTEIN LIGASE; 1.
DR PANTHER; PTHR11254:SF446; HECT-TYPE E3 UBIQUITIN TRANSFERASE; 1.
DR Pfam; PF00632; HECT; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS50237; HECT; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000018817};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 349..686
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT ACT_SITE 653
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 686 AA; 77416 MW; E64743DE52ABBFCC CRC64;
MEAVLGFLVV LAIIALLFFF LSMLARRAYI RRQRSLELHA PLLNSDDRRL DAALRDAEQG
FNECTVCSFE NFKRFKFCTV CGEAIVSDEQ DGDRDMNISK KRSTRLSFLP LLKRETKVEL
VTVTPPEQTT RRRLRAQRRK EWTRKIDVAG CAFWFRDGRW GDTQFTGFVV KFQSTPAMAD
NGMEKLLPNA VGEGESSLPP SECGTDVVTV ASSVKMDTPD PNPVSAEVNT ESVEQDSTQQ
PLVGSIDGCI ATLIRGTQEQ HVVLTPSTDT TVDASKLPLG NSTSTEDNAS TPLTEIMALA
AKDFPSKYAH FVTATSALLI PAEKEHLRLN VERANVFEDS MESLAIIPLQ NVHSVLRINF
LDESGVDAGG LHREWFVLLN EMLVDPARGL FVCTNENEQT YFLNPCFTAN SLPDQLLHLY
ATGRLLGRAL LEGSMLCFHL APPLLKLMLG YPLSFADVED LDPEVYTNLR WLLDNENVES
IGLYFTVTVK EEEKYRDVEL VPGGSSIAVT DSNKREYVER KWQYLLVESV APQLQVFLCG
LYEIVPRELL LLFDPEEFDF LLCGSDEIDV DDWERNTKYS EELHHHRSLI WFWELVREMP
NEYRRRLLQF STGSSRVPLG GFSALTSYDG RLCPFTLKAV SLVGDGFIHS HACFNRLDLP
LHVVRDELKA VLYATLETEV YGFTTD
//
