ID W2SH42_NECAM Unreviewed; 847 AA.
AC W2SH42;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE RecName: Full=ShKT domain-containing protein {ECO:0000259|PROSITE:PS51670};
GN ORFNames=NECAME_05442 {ECO:0000313|EMBL:ETN68853.1};
OS Necator americanus (Human hookworm).
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Strongyloidea; Ancylostomatidae; Bunostominae;
OC Necator.
OX NCBI_TaxID=51031 {ECO:0000313|EMBL:ETN68853.1, ECO:0000313|Proteomes:UP000053676};
RN [1] {ECO:0000313|Proteomes:UP000053676}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=24441737; DOI=10.1038/ng.2875;
RA Tang Y.T., Gao X., Rosa B.A., Abubucker S., Hallsworth-Pepin K., Martin J.,
RA Tyagi R., Heizer E., Zhang X., Bhonagiri-Palsikar V., Minx P., Warren W.C.,
RA Wang Q., Zhan B., Hotez P.J., Sternberg P.W., Dougall A., Gaze S.T.,
RA Mulvenna J., Sotillo J., Ranganathan S., Rabelo E.M., Wilson R.K.,
RA Felgner P.L., Bethony J., Hawdon J.M., Gasser R.B., Loukas A., Mitreva M.;
RT "Genome of the human hookworm Necator americanus.";
RL Nat. Genet. 46:261-269(2014).
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|ARBA:ARBA00001947};
CC -!- SIMILARITY: Belongs to the peptidase M14 family.
CC {ECO:0000256|ARBA:ARBA00005988}.
CC -!- CAUTION: Lacks conserved residue(s) required for the propagation of
CC feature annotation. {ECO:0000256|PROSITE-ProRule:PRU01005}.
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DR EMBL; KI669191; ETN68853.1; -; Genomic_DNA.
DR RefSeq; XP_013291080.1; XM_013435626.1.
DR AlphaFoldDB; W2SH42; -.
DR MEROPS; M14.A28; -.
DR EnsemblMetazoa; NECAME_05442; NECAME_05442; NECAME_05442.
DR GeneID; 25345474; -.
DR KEGG; nai:NECAME_05442; -.
DR CTD; 25345474; -.
DR OMA; DPREINC; -.
DR OrthoDB; 2872258at2759; -.
DR Proteomes; UP000053676; Unassembled WGS sequence.
DR GO; GO:0016020; C:membrane; IEA:UniProtKB-KW.
DR GO; GO:0004181; F:metallocarboxypeptidase activity; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd03860; M14_CP_A-B_like; 1.
DR Gene3D; 1.10.10.1940; -; 1.
DR Gene3D; 3.30.70.340; Metallocarboxypeptidase-like; 2.
DR Gene3D; 3.40.630.10; Zn peptidases; 2.
DR InterPro; IPR036990; M14A-like_propep.
DR InterPro; IPR003146; M14A_act_pep.
DR InterPro; IPR000834; Peptidase_M14.
DR InterPro; IPR003582; ShKT_dom.
DR PANTHER; PTHR11705; PROTEASE FAMILY M14 CARBOXYPEPTIDASE A,B; 1.
DR PANTHER; PTHR11705:SF154; SHKT DOMAIN-CONTAINING PROTEIN; 1.
DR Pfam; PF00246; Peptidase_M14; 2.
DR Pfam; PF02244; Propep_M14; 2.
DR Pfam; PF01549; ShK; 1.
DR PRINTS; PR00765; CRBOXYPTASEA.
DR SMART; SM00254; ShKT; 1.
DR SMART; SM00631; Zn_pept; 2.
DR SUPFAM; SSF54897; Protease propeptides/inhibitors; 2.
DR SUPFAM; SSF53187; Zn-dependent exopeptidases; 2.
DR PROSITE; PS00132; CARBOXYPEPT_ZN_1; 1.
DR PROSITE; PS00133; CARBOXYPEPT_ZN_2; 1.
DR PROSITE; PS51670; SHKT; 1.
PE 3: Inferred from homology;
KW Carboxypeptidase {ECO:0000256|ARBA:ARBA00022645};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049};
KW Protease {ECO:0000256|ARBA:ARBA00022670};
KW Reference proteome {ECO:0000313|Proteomes:UP000053676};
KW Signal {ECO:0000256|ARBA:ARBA00022729};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius};
KW Zinc {ECO:0000256|ARBA:ARBA00022833}.
FT TRANSMEM 582..604
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 475..508
FT /note="ShKT"
FT /evidence="ECO:0000259|PROSITE:PS51670"
SQ SEQUENCE 847 AA; 98933 MW; 61F34DF1E007E251 CRC64;
MSKKKKKGKS YRTFFFHYLF SLIVLDPVRR CVQLDFWKSP TEAGKEVHVM VNDESEDSFM
EALDSRNISH SVMIDDVKKV IVDQKEKHEK LKSQLRMRDW RDGKVQFNLA QYHSFADVIN
YMNALAITYP ERVSVQPIGT SHEGRQIPLI KITNKRLGGH KKGIWVDGGI HAREWVSPST
VLYFIHVLVT QYDKDPQVRQ FVDQIEWYIV PLLNPDGYEY SRSSSDPEIR LWRKNRSPPK
CIQQSTGLFS PPRTACCQGV DLNRNFDWFF GQVGSSTDPC SEIYQGAYAF SEPETAAVRD
FLQRHKVHTF LTFHSYSQIL MYPFGHQVRT YSNDLNDLRS TALTASAHLR RMFGTQYKVG
TGADTLYPAS GGSEDWAKGK GHVKYSYLFE LRPEEQVWDG FLLAENQIMP TARETWEAVK
VIATQTLAIP TVARAPARHV SNSPKFLHST TFFHYNRSLL SDFKSDHTPF QSRNCVDRDA
LCGSWAQGGA CGTWPEMRQR CPRSCGFCSK TTKKNFRFLC TYFPTILISW SVHYKLNFWK
TAKNLNGFWD VMVDKNVGAS LLQHLDKHEI AYLKTVNDVQ KFVLRFLFSW FLFYYFPAML
HRFFPFPIFF STKQFEKNMY RLIETKEKRE WPRRLHDDSS APFYDFHRYG SYSQMVSWMR
ALARNDPQHV QFISIGISHE GRSIDGVEIG SRDRTKRIFW IDGGIHAREW AAPHTALYFI
HQLTSRHQTD PSIKKLLEEI TFVIVPCVNP DGYEFTRSST NPHVNFKYQD LFNEYTNDCL
YPQIRLWRKN RSSMQCRKDS WGRNRCCRGV DLNRNFDFHF KGSFKDFKDA VLELCISCEV
LQSIIHL
//
