ID W2SX74_NECAM Unreviewed; 640 AA.
AC W2SX74;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 32.
DE RecName: Full=phosphoenolpyruvate carboxykinase (GTP) {ECO:0000256|ARBA:ARBA00012306};
DE EC=4.1.1.32 {ECO:0000256|ARBA:ARBA00012306};
GN ORFNames=NECAME_13189 {ECO:0000313|EMBL:ETN74133.1};
OS Necator americanus (Human hookworm).
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Strongyloidea; Ancylostomatidae; Bunostominae;
OC Necator.
OX NCBI_TaxID=51031 {ECO:0000313|EMBL:ETN74133.1, ECO:0000313|Proteomes:UP000053676};
RN [1] {ECO:0000313|Proteomes:UP000053676}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=24441737; DOI=10.1038/ng.2875;
RA Tang Y.T., Gao X., Rosa B.A., Abubucker S., Hallsworth-Pepin K., Martin J.,
RA Tyagi R., Heizer E., Zhang X., Bhonagiri-Palsikar V., Minx P., Warren W.C.,
RA Wang Q., Zhan B., Hotez P.J., Sternberg P.W., Dougall A., Gaze S.T.,
RA Mulvenna J., Sotillo J., Ranganathan S., Rabelo E.M., Wilson R.K.,
RA Felgner P.L., Bethony J., Hawdon J.M., Gasser R.B., Loukas A., Mitreva M.;
RT "Genome of the human hookworm Necator americanus.";
RL Nat. Genet. 46:261-269(2014).
CC -!- COFACTOR:
CC Name=Mn(2+); Xref=ChEBI:CHEBI:29035;
CC Evidence={ECO:0000256|ARBA:ARBA00001936};
CC -!- SIMILARITY: Belongs to the phosphoenolpyruvate carboxykinase [GTP]
CC family. {ECO:0000256|ARBA:ARBA00005796}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; KI660386; ETN74133.1; -; Genomic_DNA.
DR RefSeq; XP_013296360.1; XM_013440906.1.
DR AlphaFoldDB; W2SX74; -.
DR STRING; 51031.W2SX74; -.
DR EnsemblMetazoa; NECAME_13189; NECAME_13189; NECAME_13189.
DR GeneID; 25353217; -.
DR KEGG; nai:NECAME_13189; -.
DR CTD; 25353217; -.
DR OMA; PIMGHWL; -.
DR OrthoDB; 5471652at2759; -.
DR Proteomes; UP000053676; Unassembled WGS sequence.
DR GO; GO:0005525; F:GTP binding; IEA:UniProtKB-KW.
DR GO; GO:0016301; F:kinase activity; IEA:UniProtKB-KW.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0004613; F:phosphoenolpyruvate carboxykinase (GTP) activity; IEA:UniProtKB-EC.
DR GO; GO:0006094; P:gluconeogenesis; IEA:InterPro.
DR GO; GO:0016310; P:phosphorylation; IEA:UniProtKB-KW.
DR CDD; cd00819; PEPCK_GTP; 1.
DR Gene3D; 3.90.228.20; -; 1.
DR Gene3D; 3.40.449.10; Phosphoenolpyruvate Carboxykinase, domain 1; 1.
DR Gene3D; 2.170.8.10; Phosphoenolpyruvate Carboxykinase, domain 2; 1.
DR HAMAP; MF_00452; PEPCK_GTP; 1.
DR InterPro; IPR018091; PEP_carboxykin_GTP_CS.
DR InterPro; IPR013035; PEP_carboxykinase_C.
DR InterPro; IPR008209; PEP_carboxykinase_GTP.
DR InterPro; IPR035077; PEP_carboxykinase_GTP_C.
DR InterPro; IPR035078; PEP_carboxykinase_GTP_N.
DR InterPro; IPR008210; PEP_carboxykinase_N.
DR PANTHER; PTHR11561; PHOSPHOENOLPYRUVATE CARBOXYKINASE; 1.
DR PANTHER; PTHR11561:SF0; PHOSPHOENOLPYRUVATE CARBOXYKINASE (GTP)-RELATED; 1.
DR Pfam; PF00821; PEPCK_GTP; 1.
DR Pfam; PF17297; PEPCK_N; 1.
DR PIRSF; PIRSF001348; PEP_carboxykinase_GTP; 2.
DR SUPFAM; SSF68923; PEP carboxykinase N-terminal domain; 1.
DR SUPFAM; SSF53795; PEP carboxykinase-like; 1.
DR PROSITE; PS00505; PEPCK_GTP; 1.
PE 3: Inferred from homology;
KW Decarboxylase {ECO:0000256|ARBA:ARBA00022793};
KW GTP-binding {ECO:0000256|ARBA:ARBA00023134};
KW Kinase {ECO:0000313|EMBL:ETN74133.1};
KW Lyase {ECO:0000256|ARBA:ARBA00023239};
KW Manganese {ECO:0000256|ARBA:ARBA00023211};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Pyruvate {ECO:0000313|EMBL:ETN74133.1};
KW Reference proteome {ECO:0000313|Proteomes:UP000053676};
KW Transferase {ECO:0000313|EMBL:ETN74133.1}.
FT DOMAIN 59..300
FT /note="Phosphoenolpyruvate carboxykinase GTP-utilising N-
FT terminal"
FT /evidence="ECO:0000259|Pfam:PF17297"
FT DOMAIN 383..635
FT /note="Phosphoenolpyruvate carboxykinase C-terminal P-loop"
FT /evidence="ECO:0000259|Pfam:PF00821"
SQ SEQUENCE 640 AA; 72148 MW; 8FA71B459C9C5E4D CRC64;
MSVDPSTLTP FKETGANASL RQISDDAFYV VNEVVMKRLG HLPIHKGDFH LLPPKVQRFV
AEKAELMRPR GIYICDGSQH EADEIIDKLV ERGMLSPLKA YENNYICRTD PKDVARVESK
TWMVTPDKYQ TVTHTPQGVE PIMGHWMSPQ DLCSELDSRF PGCMSGRIMY VIPFSMGPIG
GPLSKIGVEL TDSNYVVLSM RIMTRVSSDV WDALGDQDFV RCIHSVGLPR PVKQRVINHW
PCNPERVLIA HRPAEREIWS FGSGYGGNSL LGGDLSDLQY FREPFGKKCF ALRIASNIAK
DEGWMAEHML IMGVTRPNGK EHFIAAAFPS ACGKTNLAML EPTLPGWKVR CVGDDIAWMK
FGDDGRLYGI NPESGFFGVA PETVDGEYFW EGLENEIKDK SVDMINWLGE KWRIGDPGVC
AHPNSRFAAP ASQCPIIHPE WESPKGVPIE AIIFGGRRPA GVPLIYETRS WLHGIFTGAC
LKSEATAAAE YKGKTVMHDP MAMRPFMGYN FGHYLQHWID LNKESRKVPR IYHVNWFRRD
ADNKFLWPGF GDNIRVIDWI IRRLDGEPDL GVETPIGIGS INTEGLPDIN WDELMSVPSS
YWKDDAKEIR KFLEEQVGPD LPPEIRAEMD AQEERINKAA
//
