ID W2T252_NECAM Unreviewed; 1188 AA.
AC W2T252;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 42.
DE RecName: Full=isoleucine--tRNA ligase {ECO:0000256|ARBA:ARBA00013165};
DE EC=6.1.1.5 {ECO:0000256|ARBA:ARBA00013165};
DE AltName: Full=Isoleucyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00032665};
GN ORFNames=NECAME_11931 {ECO:0000313|EMBL:ETN76085.1};
OS Necator americanus (Human hookworm).
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Strongyloidea; Ancylostomatidae; Bunostominae;
OC Necator.
OX NCBI_TaxID=51031 {ECO:0000313|EMBL:ETN76085.1, ECO:0000313|Proteomes:UP000053676};
RN [1] {ECO:0000313|Proteomes:UP000053676}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=24441737; DOI=10.1038/ng.2875;
RA Tang Y.T., Gao X., Rosa B.A., Abubucker S., Hallsworth-Pepin K., Martin J.,
RA Tyagi R., Heizer E., Zhang X., Bhonagiri-Palsikar V., Minx P., Warren W.C.,
RA Wang Q., Zhan B., Hotez P.J., Sternberg P.W., Dougall A., Gaze S.T.,
RA Mulvenna J., Sotillo J., Ranganathan S., Rabelo E.M., Wilson R.K.,
RA Felgner P.L., Bethony J., Hawdon J.M., Gasser R.B., Loukas A., Mitreva M.;
RT "Genome of the human hookworm Necator americanus.";
RL Nat. Genet. 46:261-269(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-
CC isoleucyl-tRNA(Ile); Xref=Rhea:RHEA:11060, Rhea:RHEA-COMP:9666,
CC Rhea:RHEA-COMP:9695, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58045, ChEBI:CHEBI:78442, ChEBI:CHEBI:78528,
CC ChEBI:CHEBI:456215; EC=6.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000114};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000256|ARBA:ARBA00005594}.
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DR EMBL; KI660251; ETN76085.1; -; Genomic_DNA.
DR RefSeq; XP_013298312.1; XM_013442858.1.
DR AlphaFoldDB; W2T252; -.
DR STRING; 51031.W2T252; -.
DR EnsemblMetazoa; NECAME_11931; NECAME_11931; NECAME_11931.
DR GeneID; 25351959; -.
DR KEGG; nai:NECAME_11931; -.
DR CTD; 25351959; -.
DR OMA; EIIVIHK; -.
DR OrthoDB; 656at2759; -.
DR Proteomes; UP000053676; Unassembled WGS sequence.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004822; F:isoleucine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IEA:InterPro.
DR GO; GO:0006428; P:isoleucyl-tRNA aminoacylation; IEA:InterPro.
DR CDD; cd07961; Anticodon_Ia_Ile_ABEc; 1.
DR CDD; cd00818; IleRS_core; 1.
DR Gene3D; 3.40.50.620; HUPs; 2.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR033709; Anticodon_Ile_ABEc.
DR InterPro; IPR002301; Ile-tRNA-ligase.
DR InterPro; IPR023586; Ile-tRNA-ligase_type2.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR NCBIfam; TIGR00392; ileS; 1.
DR PANTHER; PTHR42780:SF1; ISOLEUCINE--TRNA LIGASE, CYTOPLASMIC; 1.
DR PANTHER; PTHR42780; SOLEUCYL-TRNA SYNTHETASE; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF19302; DUF5915; 1.
DR Pfam; PF00133; tRNA-synt_1; 2.
DR PRINTS; PR00984; TRNASYNTHILE.
DR SUPFAM; SSF47323; Anticodon-binding domain of a subclass of class I aminoacyl-tRNA synthetases; 1.
DR SUPFAM; SSF52374; Nucleotidylyl transferase; 1.
DR SUPFAM; SSF50677; ValRS/IleRS/LeuRS editing domain; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase {ECO:0000256|ARBA:ARBA00023146};
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Ligase {ECO:0000256|ARBA:ARBA00022598, ECO:0000313|EMBL:ETN76085.1};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Protein biosynthesis {ECO:0000256|ARBA:ARBA00022917};
KW Reference proteome {ECO:0000313|Proteomes:UP000053676}.
FT DOMAIN 20..67
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 90..662
FT /note="Aminoacyl-tRNA synthetase class Ia"
FT /evidence="ECO:0000259|Pfam:PF00133"
FT DOMAIN 714..864
FT /note="Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase
FT anticodon-binding"
FT /evidence="ECO:0000259|Pfam:PF08264"
SQ SEQUENCE 1188 AA; 136971 MW; 534FBD699359FCF4 CRC64;
MSGLITVPDT INFPSEEEKT LKIWNENKIF EKSLKLASDR PRYTFFDGPP FATGLPHYGH
ILAGTIKVFS KFLELEPLRK CRNDTGFPFQ DVVTRWAHQD GYYVERRFGW DTHGLPVEYE
VDKKLGIKGP SDILEMGIGK YNAECRSIVM RYSHEWETMV ARMGRWIDFR NDYKTLYPWF
MESVWWAFSE LFKKGLVYRG VKVMPYSTSC TTPLSNFEAG QNYKDVVDPC VCVGFRLDEN
QNRMMVAWTT TPWTLPSNLA LCVHPDLDYV VVRDPGSGME YVVLEERLFE LKNEHLELIE
KVKGRVFEGK AYEPLFPYFS YMKEERGAFR VLCNTFVTTD QGTGVVHQAP YFGEIDYQIC
LENGVITKDM KIICPVDESG RFTKEVTDFA NLYVKDADKQ ICKNLKERGY LIKQGEVKHS
YPFCWRSDTP LLYKAVPSWF IRVEQIIPKL LANNDQTYWV PSFVKEKRFA NWLRDARDWA
VSRNRFWGTP INLWVSDDLE EIVCPGSIAE LESLTGTKIT DIHRESVDHL TIPSKTGRGV
LKRVTEVFDC WFESGSMPYA QNHYPFENKK IFEDNFPADF IAEGIDQTRG WFYTLLVLST
ALFNKPPFKN LICNGLVLAA DGNKMSKRKK NYPDPMEVVQ KYGADALRLY LINSPVVRGE
NLRFREEGVR DLLKDVFLPW FNAYRFFVQN VQLYEHETGS VFSMKDTISE NVMDRWIESF
TNSLVRFVRK EMSQYRLYAV VTPLTRFFDT LTNCYIRLNR KRIKGDFGAN DQTHALSALG
RVLIIIVRLM SPFTPYFCEY VWQNLRKVTG ATEESVHFTM LPTPDDSLID ESVERRVQAM
RDCIDLVRVL RERKGIPVKY PLKEMIVVNR ERQFLDDLRS LEHYILSEVN VRKLTVSQDK
QKYGITLKAS FSESLDKHLC MLFTAEPNFR LLGTRLKGDQ KKVVDYLKTQ IKESELEDFL
ETGWFIILVL WKLTVFGHEL SSEEVYVSYT SGQGGAKDHG YETHSDGKTI VMLDTSEDSS
LVEEGLAREI TNRVQKLRKA AKLVSTDPAV VYCVVKPETT QLATVVGSYK EKIEEATGTP
MRLELLPSDK SATVSSVSTV KDAEVSLWLV VSDALKDVVT VGCNGKTVRI HLKSGSEKML
SYNDLLYEIR SALDLWEGKF WLMLSDGTRF HPTASVEALS GQAVKLEF
//
