ID W2T2Q4_NECAM Unreviewed; 735 AA.
AC W2T2Q4;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 50.
DE RecName: Full=phosphatidylinositol-3-phosphatase {ECO:0000256|ARBA:ARBA00013038};
DE EC=3.1.3.64 {ECO:0000256|ARBA:ARBA00013038};
DE AltName: Full=Phosphatidylinositol-3-phosphate phosphatase {ECO:0000256|ARBA:ARBA00031219};
GN ORFNames=NECAME_12087 {ECO:0000313|EMBL:ETN75834.1};
OS Necator americanus (Human hookworm).
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Strongyloidea; Ancylostomatidae; Bunostominae;
OC Necator.
OX NCBI_TaxID=51031 {ECO:0000313|EMBL:ETN75834.1, ECO:0000313|Proteomes:UP000053676};
RN [1] {ECO:0000313|Proteomes:UP000053676}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=24441737; DOI=10.1038/ng.2875;
RA Tang Y.T., Gao X., Rosa B.A., Abubucker S., Hallsworth-Pepin K., Martin J.,
RA Tyagi R., Heizer E., Zhang X., Bhonagiri-Palsikar V., Minx P., Warren W.C.,
RA Wang Q., Zhan B., Hotez P.J., Sternberg P.W., Dougall A., Gaze S.T.,
RA Mulvenna J., Sotillo J., Ranganathan S., Rabelo E.M., Wilson R.K.,
RA Felgner P.L., Bethony J., Hawdon J.M., Gasser R.B., Loukas A., Mitreva M.;
RT "Genome of the human hookworm Necator americanus.";
RL Nat. Genet. 46:261-269(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-inositol-3-
CC phosphate) + H2O = a 1,2-diacyl-sn-glycero-3-phospho-(1D-myo-
CC inositol) + phosphate; Xref=Rhea:RHEA:12316, ChEBI:CHEBI:15377,
CC ChEBI:CHEBI:43474, ChEBI:CHEBI:57880, ChEBI:CHEBI:58088; EC=3.1.3.64;
CC Evidence={ECO:0000256|ARBA:ARBA00001291};
CC -!- SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Non-
CC receptor class myotubularin subfamily. {ECO:0000256|ARBA:ARBA00007471}.
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DR EMBL; KI660268; ETN75834.1; -; Genomic_DNA.
DR RefSeq; XP_013298061.1; XM_013442607.1.
DR AlphaFoldDB; W2T2Q4; -.
DR STRING; 51031.W2T2Q4; -.
DR EnsemblMetazoa; NECAME_12087; NECAME_12087; NECAME_12087.
DR GeneID; 25352115; -.
DR KEGG; nai:NECAME_12087; -.
DR CTD; 25352115; -.
DR OMA; QWQHTDV; -.
DR OrthoDB; 5474662at2759; -.
DR Proteomes; UP000053676; Unassembled WGS sequence.
DR GO; GO:0004438; F:phosphatidylinositol-3-phosphate phosphatase activity; IEA:UniProtKB-EC.
DR GO; GO:0016311; P:dephosphorylation; IEA:InterPro.
DR Gene3D; 2.30.29.30; Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB); 1.
DR InterPro; IPR010569; Myotubularin-like_Pase_dom.
DR InterPro; IPR030564; Myotubularin_fam.
DR InterPro; IPR011993; PH-like_dom_sf.
DR InterPro; IPR029021; Prot-tyrosine_phosphatase-like.
DR InterPro; IPR016130; Tyr_Pase_AS.
DR InterPro; IPR003595; Tyr_Pase_cat.
DR PANTHER; PTHR10807; MYOTUBULARIN-RELATED; 1.
DR PANTHER; PTHR10807:SF8; PHOSPHATIDYLINOSITOL-3-PHOSPHATE PHOSPHATASE; 1.
DR Pfam; PF06602; Myotub-related; 1.
DR Pfam; PF21098; PH-GRAM_MTMR6-like; 2.
DR SMART; SM00404; PTPc_motif; 1.
DR SUPFAM; SSF52799; (Phosphotyrosine protein) phosphatases II; 1.
DR SUPFAM; SSF50729; PH domain-like; 1.
DR PROSITE; PS51339; PPASE_MYOTUBULARIN; 1.
DR PROSITE; PS00383; TYR_PHOSPHATASE_1; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000053676}.
FT DOMAIN 197..572
FT /note="Myotubularin phosphatase"
FT /evidence="ECO:0000259|PROSITE:PS51339"
FT REGION 628..667
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 628..642
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 406
FT /note="Phosphocysteine intermediate"
FT /evidence="ECO:0000256|PIRSR:PIRSR630564-1"
FT BINDING 345..346
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR630564-2"
FT BINDING 406..412
FT /ligand="substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR630564-2"
SQ SEQUENCE 735 AA; 83904 MW; 07FE92329C0F2F27 CRC64;
MTFSSYPAYT EDISVAGSLL RDKISGLYES IVKRERRWSD WDLASSWRII MKVDKVQLVD
RLGVEPNVIG TLHVTTSHII FRSEEGSKEL WVWRVSVIYA LGKLFREAKV GFESPSISQQ
SLQIANGLIG SVERGPLSAA GCPLIIRCKH FQVINLLIAR DKICQDLFET LLRCSKTVNV
CELVAFENRD VAEDARGWAR LDWGVEFTRQ GVDSEWCEND LNESYRSCDT YPERLWLPVT
ANKTTLLGSC RFRSRGRLPV LTYFHKSNGS AICRCAQPLT GFSARCVEDE KLMELIGRAN
KNCDTLFLVD TRPMVNAMVN KVQGKGFEDE RNYSNTRFHF FDIENIHVMR NSQQKLLEAC
YKCRTMSEYL RTLESSGWLK HLRAIVECAT FLADSISRGI SCVVHCSDGW DRTSQTVSLA
QLLLDPFFRT IHGFQVLIEK DWLGFGHKFD DRCAHIGALN DEAAKEVSPI FSQFLDCVHQ
VMCQRPRAFQ FNDRYLIDIH EHVYSCQFGT FIGNCEKDRK DLHIDRRTKS LWTYLDHRQD
DYLNPFYEPT TFGVLSGIET RAFAFKVWHG LYNRFDDGLL PKETMTDMVM TAMEHVGVLE
SHIAALRIRI AELKCQLGRG GTCCGNSMAD SGHSSGTGQS EERLAPDQRP PSRESGVYEQ
SMSHSSSEGI GISTFFKEEA LTRLPVRWQP LRDASHCTSK SCRAEFASAV DRRIHCHRCG
KVSVRALWEF MKIMK
//
