ID   W3X840_PESFW            Unreviewed;       523 AA.
AC   W3X840;
DT   19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT   19-MAR-2014, sequence version 1.
DT   24-JAN-2024, entry version 42.
DE   RecName: Full=Isocitrate lyase {ECO:0000256|PIRNR:PIRNR001362};
GN   ORFNames=PFICI_06343 {ECO:0000313|EMBL:ETS81341.1};
OS   Pestalotiopsis fici (strain W106-1 / CGMCC3.15140).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Xylariomycetidae; Xylariales; Sporocadaceae; Pestalotiopsis.
OX   NCBI_TaxID=1229662 {ECO:0000313|EMBL:ETS81341.1, ECO:0000313|Proteomes:UP000030651};
RN   [1] {ECO:0000313|Proteomes:UP000030651}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=W106-1 / CGMCC3.15140 {ECO:0000313|Proteomes:UP000030651};
RX   PubMed=25623211; DOI=10.1186/s12864-014-1190-9;
RA   Wang X., Zhang X., Liu L., Xiang M., Wang W., Sun X., Che Y., Guo L.,
RA   Liu G., Guo L., Wang C., Yin W.B., Stadler M., Zhang X., Liu X.;
RT   "Genomic and transcriptomic analysis of the endophytic fungus
RT   Pestalotiopsis fici reveals its lifestyle and high potential for synthesis
RT   of natural products.";
RL   BMC Genomics 16:28-28(2015).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate +
CC         succinate; Xref=Rhea:RHEA:16809, ChEBI:CHEBI:15361,
CC         ChEBI:CHEBI:30031, ChEBI:CHEBI:57429; EC=4.1.3.30;
CC         Evidence={ECO:0000256|ARBA:ARBA00001050};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000256|PIRSR:PIRSR001362-3};
CC       Note=Can also use Mn(2+) ion. {ECO:0000256|PIRSR:PIRSR001362-3};
CC   -!- SUBUNIT: Homotetramer. {ECO:0000256|ARBA:ARBA00011881}.
CC   -!- SIMILARITY: Belongs to the isocitrate lyase/PEP mutase superfamily.
CC       Isocitrate lyase family. {ECO:0000256|ARBA:ARBA00005704,
CC       ECO:0000256|PIRNR:PIRNR001362}.
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DR   EMBL; KI912112; ETS81341.1; -; Genomic_DNA.
DR   RefSeq; XP_007833115.1; XM_007834924.1.
DR   AlphaFoldDB; W3X840; -.
DR   STRING; 1229662.W3X840; -.
DR   GeneID; 19271356; -.
DR   KEGG; pfy:PFICI_06343; -.
DR   eggNOG; KOG1260; Eukaryota.
DR   HOGENOM; CLU_019214_2_2_1; -.
DR   InParanoid; W3X840; -.
DR   OMA; GTLTGCM; -.
DR   OrthoDB; 1981645at2759; -.
DR   Proteomes; UP000030651; Unassembled WGS sequence.
DR   GO; GO:0004451; F:isocitrate lyase activity; IEA:InterPro.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0046421; F:methylisocitrate lyase activity; IEA:UniProtKB-EC.
DR   GO; GO:0019752; P:carboxylic acid metabolic process; IEA:InterPro.
DR   Gene3D; 1.10.10.850; -; 1.
DR   Gene3D; 3.20.20.60; Phosphoenolpyruvate-binding domains; 1.
DR   InterPro; IPR006254; Isocitrate_lyase.
DR   InterPro; IPR015813; Pyrv/PenolPyrv_Kinase-like_dom.
DR   InterPro; IPR040442; Pyrv_Kinase-like_dom_sf.
DR   NCBIfam; TIGR01346; isocit_lyase; 1.
DR   PANTHER; PTHR21631:SF3; BIFUNCTIONAL GLYOXYLATE CYCLE PROTEIN; 1.
DR   PANTHER; PTHR21631; ISOCITRATE LYASE/MALATE SYNTHASE; 1.
DR   Pfam; PF00463; ICL; 1.
DR   PIRSF; PIRSF001362; Isocit_lyase; 2.
DR   SUPFAM; SSF51621; Phosphoenolpyruvate/pyruvate domain; 1.
PE   3: Inferred from homology;
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|PIRNR:PIRNR001362};
KW   Magnesium {ECO:0000256|PIRSR:PIRSR001362-3};
KW   Metal-binding {ECO:0000256|PIRSR:PIRSR001362-3};
KW   Reference proteome {ECO:0000313|Proteomes:UP000030651}.
FT   BINDING         172
FT                   /ligand="Mg(2+)"
FT                   /ligand_id="ChEBI:CHEBI:18420"
FT                   /evidence="ECO:0000256|PIRSR:PIRSR001362-3"
SQ   SEQUENCE   523 AA;  58906 MW;  46C158A3D900C1C1 CRC64;
     MAQKYADIQS EKDAFAAEVE SIKQWWQTDR QKHIQRPYTA ESIAALRNIG FKIEYPSSAQ
     GRKLWRLLNE HNEKGTYDLT FGTTDPLIAK EMPKAGVQTV YVSGALCGLS QAAWPGADHA
     DYPADLVPSV VKRILNTQLF HDQRQTQLRM RYNEEDRKSL ENLDYLLPIV ADADMGFGTL
     TGCMKLTRSF VEAGVAMIHI DDLALGLKKF TNGEGRTIVP TREYLDRLTT VRMTFDIMGA
     DTMLLCRCDS DNAEFITSVI DPRDHPYVLG ATKQVPTFIQ ALDEGKIAGK DYLTVKSEWK
     AAAALMTFDE AVKAVASDEQ YTAYMSEVND KIVALQERKA IAKRVVGYEI TFDWELPRLP
     TGQYMWKWCT KAVIDRCILA APLGDVTWSR QDKPNKKDMH DFHTSVRKVF PGRLFAFGYT
     GAYDYTKNGY TQAEMESFPA DIAKLGCVWQ VQPIWATQGL SLHAKEFAES FKKGGIAWYM
     RDVAAPAREK MATDKYGKPQ ARGDYLADAF FDVVAGLDIV EKA
//