ID W4E019_9BACL Unreviewed; 1195 AA.
AC W4E019;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE SubName: Full=Ribonucleoside-diphosphate reductase {ECO:0000313|EMBL:ETT73307.1};
GN ORFNames=C173_12045 {ECO:0000313|EMBL:ETT73307.1};
OS Paenibacillus sp. FSL R7-277.
OC Bacteria; Bacillota; Bacilli; Bacillales; Paenibacillaceae; Paenibacillus.
OX NCBI_TaxID=1227352 {ECO:0000313|EMBL:ETT73307.1, ECO:0000313|Proteomes:UP000019051};
RN [1] {ECO:0000313|EMBL:ETT73307.1, ECO:0000313|Proteomes:UP000019051}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=FSL R7-277 {ECO:0000313|EMBL:ETT73307.1,
RC ECO:0000313|Proteomes:UP000019051};
RX PubMed=24422886; DOI=10.1186/1471-2164-15-26;
RA Moreno Switt A.I., Andrus A.D., Ranieri M.L., Orsi R.H., Ivy R.,
RA den Bakker H.C., Martin N.H., Wiedmann M., Boor K.J.;
RT "Genomic comparison of sporeforming bacilli isolated from milk.";
RL BMC Genomics 15:26-26(2014).
CC -!- COFACTOR:
CC Name=adenosylcob(III)alamin; Xref=ChEBI:CHEBI:18408;
CC Evidence={ECO:0000256|ARBA:ARBA00001922};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:ETT73307.1}.
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DR EMBL; ASPX01000033; ETT73307.1; -; Genomic_DNA.
DR RefSeq; WP_063830904.1; NZ_ASPX01000033.1.
DR AlphaFoldDB; W4E019; -.
DR PATRIC; fig|1227352.4.peg.2408; -.
DR eggNOG; COG0209; Bacteria.
DR eggNOG; COG1372; Bacteria.
DR OrthoDB; 9762933at2; -.
DR Proteomes; UP000019051; Unassembled WGS sequence.
DR GO; GO:0031419; F:cobalamin binding; IEA:UniProtKB-KW.
DR GO; GO:0004519; F:endonuclease activity; IEA:InterPro.
DR GO; GO:0016491; F:oxidoreductase activity; IEA:UniProtKB-KW.
DR GO; GO:0016539; P:intein-mediated protein splicing; IEA:InterPro.
DR CDD; cd00081; Hint; 1.
DR Gene3D; 3.20.70.20; -; 2.
DR Gene3D; 2.170.16.10; Hedgehog/Intein (Hint) domain; 1.
DR Gene3D; 3.10.28.10; Homing endonucleases; 1.
DR InterPro; IPR003586; Hint_dom_C.
DR InterPro; IPR003587; Hint_dom_N.
DR InterPro; IPR036844; Hint_dom_sf.
DR InterPro; IPR027434; Homing_endonucl.
DR InterPro; IPR006142; INTEIN.
DR InterPro; IPR030934; Intein_C.
DR InterPro; IPR004042; Intein_endonuc.
DR InterPro; IPR006141; Intein_N.
DR InterPro; IPR004860; LAGLIDADG_2.
DR InterPro; IPR000788; RNR_lg_C.
DR NCBIfam; TIGR01445; intein_Nterm; 1.
DR PANTHER; PTHR43371:SF1; RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE; 1.
DR PANTHER; PTHR43371; VITAMIN B12-DEPENDENT RIBONUCLEOTIDE REDUCTASE; 1.
DR Pfam; PF14890; Intein_splicing; 1.
DR Pfam; PF14528; LAGLIDADG_3; 1.
DR Pfam; PF02867; Ribonuc_red_lgC; 1.
DR PRINTS; PR00379; INTEIN.
DR SMART; SM00305; HintC; 1.
DR SMART; SM00306; HintN; 1.
DR SUPFAM; SSF51294; Hedgehog/intein (Hint) domain; 1.
DR SUPFAM; SSF51998; PFL-like glycyl radical enzymes; 1.
DR PROSITE; PS50818; INTEIN_C_TER; 1.
DR PROSITE; PS50819; INTEIN_ENDONUCLEASE; 1.
DR PROSITE; PS50817; INTEIN_N_TER; 1.
PE 4: Predicted;
KW Autocatalytic cleavage {ECO:0000256|ARBA:ARBA00022813};
KW Cobalamin {ECO:0000256|ARBA:ARBA00022628};
KW Cobalt {ECO:0000256|ARBA:ARBA00023285};
KW Oxidoreductase {ECO:0000256|ARBA:ARBA00023002};
KW Protein splicing {ECO:0000256|ARBA:ARBA00023000}.
FT DOMAIN 473..555
FT /note="DOD-type homing endonuclease"
FT /evidence="ECO:0000259|PROSITE:PS50819"
FT DOMAIN 643..665
FT /note="Intein C-terminal splicing"
FT /evidence="ECO:0000259|PROSITE:PS50818"
FT REGION 955..981
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 955..970
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1195 AA; 131310 MW; C816F82EC034C544 CRC64;
MSTVEHNKRL EGLSEKIFLD RYAWKDADSN NAKVGDVVLV LTKDDPKFPT KEVGEIVERT
GRMVTVKTRS GELVQSDVEK LTLNIEKTPE EMWDRLAAAM ASVEKTPGLQ EEWTGKFRSI
LDDWKLVPGG RIAAGAGASE ELTLFNCYVI PSPKDSRGGI METLSEMTEI MARGGGVGIN
LSSLRPRRAI VRGVNGSSSG SVSWGGLFSY TTGLIEQGGS RRGALMLMIN DWHPDVLDFI
TVKQTMGQVT NANLSVCVSN GFMKAVKEDL DWELVFPDTT DPDYDALWDG DLDKWKSDNR
RVIPYRTVRA RDIWHTIIES AWKSAEPGVV FMEYYNQMSN SWYFNPIICT NPCFHPNTRI
ATEYGLLTIE ELYKKVGTES FLVGTDSRLV EQAKVVGGRS YEVPGLQMRR ATVFPTGTRE
TLKISLQNGV ELSVTPEHRL FTDSGWKEAQ TLSADDCVYL QSGEGGFAAK DTLGEGWVNE
QLEELGVKAV RAAEKEVPAA LYTASRATVV AFLQGLFSAD GTVYENDEMH RTVRLTSASK
KLLQGVQLLL LNFGINSSIY NRQKKNQAIF TYTTIAGEER SYEGSGFYEL ILSGNNILEF
KNKIGFKLIT RKQEALGRIA RPSRKNEKFI SKVVGIEAGE VVTVYDITEP VTHSLIAGGV
VAHNCGEQGL PGWGVCNLSA VNLSKFYDAE KHDVDWADLA TTTRYSVRFL DNVIDKTPYH
FPENEANQKL ERRVGLGTMG LAELMIKLNI RYGSPESLEF LDKLYGFMAR EAYLASAEIA
GEKGSFQAFD TDKYLQSGFM RNITEVYPEV SESIRKHGMR NVTVITQAPT GSTGTMVGTS
TGIEPYFAFK YFRQSRLGYD EQFVPIAQEW LEAHPGEELP DYFVTSMDLS AKDHIRAQAA
IQRWVDSSIS KTANCPSDFT VEETAELYEM AFDLGCKGVT IYRDGSRDVQ VLETKKKEDK
KDEPATAAKP EVIKETEAAP SAPTTVAAAP AHQEAASGVD KQYKKRPQVL RGATYKINTP
FGMAYITIND LDGIPAEIFL NVGKAGSDVF AMAEALGRVC SLFLRYGDHG EKVGLLIKHL
KGIGGSGAIG FGANRVESIA DAVAKALETH VLNNAHDDHI PAPIAATLEL DFNAELNAEL
KSTVPAAATN DSHGGHDAHS HATASRDLCP SCGSASLINI EGCKTCGNCG YSRCG
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