ID W4IST4_PLAFP Unreviewed; 2909 AA.
AC W4IST4;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 46.
DE RecName: Full=DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00012417};
DE EC=2.7.7.7 {ECO:0000256|ARBA:ARBA00012417};
GN ORFNames=PFUGPA_04659 {ECO:0000313|EMBL:ETW53033.1};
OS Plasmodium falciparum (isolate Palo Alto / Uganda).
OC Eukaryota; Sar; Alveolata; Apicomplexa; Aconoidasida; Haemosporida;
OC Plasmodiidae; Plasmodium; Plasmodium (Laverania).
OX NCBI_TaxID=57270 {ECO:0000313|EMBL:ETW53033.1, ECO:0000313|Proteomes:UP000019103};
RN [1] {ECO:0000313|EMBL:ETW53033.1, ECO:0000313|Proteomes:UP000019103}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Palo Alto/Uganda {ECO:0000313|EMBL:ETW53033.1,
RC ECO:0000313|Proteomes:UP000019103};
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Neafsey D., Hoffman S., Volkman S., Rosenthal P., Walker B., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Allen A.W.,
RA Alvarado L., Arachchi H.M., Berlin A.M., Chapman S.B., Gainer-Dewar J.,
RA Goldberg J., Griggs A., Gujja S., Hansen M., Howarth C., Imamovic A.,
RA Ireland A., Larimer J., McCowan C., Murphy C., Pearson M., Poon T.W.,
RA Priest M., Roberts A., Saif S., Shea T., Sisk P., Sykes S., Wortman J.,
RA Nusbaum C., Birren B.;
RT "The Genome Annotation of Plasmodium falciparum Palo Alto/Uganda.";
RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|EMBL:ETW53033.1, ECO:0000313|Proteomes:UP000019103}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Palo Alto/Uganda {ECO:0000313|EMBL:ETW53033.1,
RC ECO:0000313|Proteomes:UP000019103};
RG The Broad Institute Genome Sequencing Platform;
RG The Broad Institute Genome Sequencing Center for Infectious Disease;
RA Neafsey D., Cheeseman I., Volkman S., Adams J., Walker B., Young S.K.,
RA Zeng Q., Gargeya S., Fitzgerald M., Haas B., Abouelleil A., Alvarado L.,
RA Arachchi H.M., Berlin A.M., Chapman S.B., Dewar J., Goldberg J., Griggs A.,
RA Gujja S., Hansen M., Howarth C., Imamovic A., Larimer J., McCowan C.,
RA Murphy C., Neiman D., Pearson M., Priest M., Roberts A., Saif S., Shea T.,
RA Sisk P., Sykes S., Wortman J., Nusbaum C., Birren B.;
RT "The Genome Sequence of Plasmodium falciparum Palo Alto/Uganda.";
RL Submitted (FEB-2013) to the EMBL/GenBank/DDBJ databases.
CC -!- COFACTOR:
CC Name=[4Fe-4S] cluster; Xref=ChEBI:CHEBI:49883;
CC Evidence={ECO:0000256|ARBA:ARBA00001966};
CC -!- SIMILARITY: Belongs to the DNA polymerase type-B family.
CC {ECO:0000256|ARBA:ARBA00005755}.
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DR EMBL; KI927386; ETW53033.1; -; Genomic_DNA.
DR EnsemblProtists; ETW53033; ETW53033; PFUGPA_04659.
DR OMA; ESYYYAS; -.
DR Proteomes; UP000019103; Unassembled WGS sequence.
DR GO; GO:0008622; C:epsilon DNA polymerase complex; IEA:InterPro.
DR GO; GO:0003677; F:DNA binding; IEA:InterPro.
DR GO; GO:0003887; F:DNA-directed DNA polymerase activity; IEA:UniProtKB-KW.
DR GO; GO:0051536; F:iron-sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0000166; F:nucleotide binding; IEA:InterPro.
DR GO; GO:0008270; F:zinc ion binding; IEA:InterPro.
DR GO; GO:0006281; P:DNA repair; IEA:InterPro.
DR GO; GO:0006260; P:DNA replication; IEA:InterPro.
DR CDD; cd05779; DNA_polB_epsilon_exo; 1.
DR Gene3D; 1.10.132.60; DNA polymerase family B, C-terminal domain; 1.
DR Gene3D; 1.10.287.690; Helix hairpin bin; 1.
DR Gene3D; 3.90.1600.10; Palm domain of DNA polymerase; 1.
DR Gene3D; 3.30.420.10; Ribonuclease H-like superfamily/Ribonuclease H; 1.
DR InterPro; IPR006172; DNA-dir_DNA_pol_B.
DR InterPro; IPR043502; DNA/RNA_pol_sf.
DR InterPro; IPR042087; DNA_pol_B_thumb.
DR InterPro; IPR013697; DNA_pol_e_suA_C.
DR InterPro; IPR023211; DNA_pol_palm_dom_sf.
DR InterPro; IPR029703; POL2.
DR InterPro; IPR012337; RNaseH-like_sf.
DR InterPro; IPR036397; RNaseH_sf.
DR PANTHER; PTHR10670:SF0; DNA POLYMERASE EPSILON CATALYTIC SUBUNIT 1; 1.
DR PANTHER; PTHR10670; DNA POLYMERASE EPSILON CATALYTIC SUBUNIT A; 1.
DR SMART; SM01159; DUF1744; 1.
DR SMART; SM00486; POLBc; 1.
DR SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR SUPFAM; SSF53098; Ribonuclease H-like; 1.
PE 3: Inferred from homology;
KW DNA-directed DNA polymerase {ECO:0000256|ARBA:ARBA00022932};
KW Iron {ECO:0000256|ARBA:ARBA00023004};
KW Iron-sulfur {ECO:0000256|ARBA:ARBA00023014};
KW Metal-binding {ECO:0000256|ARBA:ARBA00023014};
KW Nucleotidyltransferase {ECO:0000256|ARBA:ARBA00022695};
KW Reference proteome {ECO:0000313|Proteomes:UP000019103};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 1967..2519
FT /note="DNA polymerase epsilon catalytic subunit A C-
FT terminal"
FT /evidence="ECO:0000259|SMART:SM01159"
FT REGION 211..259
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 793..816
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1534..1554
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1620..1661
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2285..2333
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 240..259
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1633..1657
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2909 AA; 344795 MW; 3FA330756E716026 CRC64;
MSTQTRTKGW EKESKINPDY EYERLLKLEN KFHIKAWINK GEKEGYLYNI VPTTMNVVAK
NSNKSHKKTG VHMYFVSDNN KTWRLTLFYR PYFYLKTKNI HNYEQVTKFL KKELDKYNVE
IDYVKKEDLS LYDHLNKKRS YLSNIFFKLS YDTIENLMNA RDFLVKIIER NKKQNKNYNN
SNNDKHIFNN EDLSYSQLEF SYKNEYSYNK YDDQKENTQK QQFTTNITEE DDKKRILNAK
SNDNNNNNNN NNTHNSNNNN NILNMNDIIV TTKKQILSKE EIMNEIIEIY EYDVKYLTRI
CIDKNIRCGL WYKVKREEDE LYTELIQFEI LNKKVLAPLN ILAWDIECYK DELKFPDKEK
DEIILISYMY NAQGYLIVNR NVMSKNIREF LYKPNEEYSG AGTFKIFNEK NEYFLLKRFL
EHIRLLKIHI FVTYNGDFFD IPYLYRRCEI NNLSVSREIG FIMNNKQECS CNFILNIDAY
KWVERDSYLP NGSRTLKSVC KIKLKYNPTE VDPELMVPIA KNNPQHLAVY SVSDAVATFY
LYDKFIHNFL FALSSIIPMN PDNVLRQGSG TLCEQLLMAE AYRKNILFPN KSKPIYNQYF
TDPENKKKYF IYDDSFVGGT VQSLKCGIYR DDLKEFFSLD VDTYKYLYNS IDHIIDFWIQ
KDLNKNANIN DTKYINKNQI INLDQIKQDI KNKLNFFIEN PNINICPNIY HLDVAAMYPN
IILSHRLQPN AIITPDHCFN CSFYKQRHLC QKKMIWKRKL EISPIDYGHV LSLKQDLKTR
LFFPQKSFYK MQNKEESDST ENENSMDINA SKKKSWNELT DKQQHDELMK VIKDCSQKVF
KKTKVSKEVD ASSLVCQREN PFYVDAVKTF RDRRYVYKKG LKECEHEKRE LLKQSKIDYI
KIQELDDKIL LNDSLQLAHK CILNSFYGYV KRRSSRWYSD QMGAIVTYTG SQIINGAFNL
INKIGIPMEL DTDGIWCMLP KNFPEMYDVY ILDKKEINKK KDYETKSEEE LKNDNNVKKV
DFEFPTNILN FEMHKKWTND QYLVYNEHTD DYECISKNEI FFELDGPWHG MFLPASEKSD
DLLKKRYVVF NNKYKISELK GFEIKRRGEL RIIQRFQSEI FNHFLKGKTK EESYYHASLT
ANKWKNLIDT KAIDIDNDDE LFDLILAKKV LNKSVKEQPN AKSFGITTAK RLSELLNNAS
YIEDNNVSTQ FIVVSKPIGS DITFRAIPIQ IFKTNIDTQI HYLSKWLGTK FPPNVPVNVR
DIIDWDYYKQ KLEVQILKLV IIPAIKQNIN NPITSISVPD WLKKQINISE GKQKKITSFF
VKKNIKKEDT TSPLHSADVK LVNDIHDFKD SNETTNTPLH YTQKESMKNK EHISNQSMEK
KSPFPIEGEN TITNPSSSIL TQSEETLKNK KRKLIKQYLL DNFISKKQKL IILYDNNQSN
NNNSNIMSGA TPKTSKLISS VAYKKINSFE NYGENNNNII IIDHEKINIS NLKYKDLHNL
FHSNFKNWML INNKIWKNNR TQIKKIRIED KKQKKKNSIS FKEQEDEETN KEKKNYEKIS
NLDSKYLHTI QNSMDIVYIY KKIKKKKKNH KKKNANIQLN NLKNDKQTLL KPYLKYFKHE
ANSDSDSNSD SNSDDSSSHK KESRNENYHI NEKVDNNDYY NDDDDDDDDG IYYALVSLKN
MNKFYKIKIQ VYRHIYINNY DQLNIKSNSK ITIKLISSNN DDNGVFKSKA YANCFLPRNI
KVFNLYEFIM TEKYFNQYII NTLNANYHES IISVYETKIP LYFDFLSRYG NSVEIDSNDY
NYIFDENKCF KSHCFSKVEN NSKSTKNVSA NYLDDVHIVY IHIFHTLIDN ICNRIFINVY
DQFDEHNQDH LLGNKIMFSG IGQKDNNFDP YDTFIKFLQM DKYCNDNTLN ETNKNINMNI
NSTHDGQNKN ILNINHIHNT FDNNNNNNND VEYLNNIEKE KIKKDMLLKN LFNKEFFINS
VEKDLLFLYH YRKFDVYYED NTNVYKVLEY LDLYLNKYRT NMITGKQKYI FYVSSTIDKK
KLGWWATNKY FPCYFYKFDN TNKYQNVPRL NYQNDIFNLS LNLFFENYHR VEEDLNFSRI
SNIPLFNLFN VQNKNQKHKF IYDILYASFI KKYKGILWLS YFGNYDLGIP CLNINNFCDY
DLAKKNNDII NQGIYRGYIV HLFFNESLIF NSVRLFTKYS NLDKNTNEYN NTITNTNTNI
NNYHNNNIIN RNNEHTTFMQ NKKKNVKRTL ENNTNHMNKL NNKSNHINKN DSIDQKEQLN
DEDISTADEN NNMNSNNNNN NNNNNNNNNN NNNNNNNNNN NNNNNSVNVN FSSNNDNVII
QSSAELNQKK NLIQNKYDIN SIVERNAHVS QFSNFAFKLL GQSLEYLISK ISSLSMLITN
KTFESISDIF TSFYSWISNN SSLLYDVALY NKVLECSEIY QNNLINIMKK KFNANIIFAD
LRNLVISFNE YSIISGRNIL KNLIKYFSHS DSIYANVPFY IKNEYIAACQ FDKYNFIRYK
KYSDPNEENT DENLKIIEYL PPICESFLRY VLDIIILNPL HDIILSYDNN KQNGINPYQS
DDQKINHIND ISTCEKNYYN VILKNDKLTD KINLAQKAKL KYIFDKSFDD LEEVCETFSL
INENVDVLAY KIEEKLKDLW FMPGIIYKKV KKAIKYKKYL IENYWPYEDD EIGENNINYA
PFLFPQTLGN LAKRDNNWRL EIVKFCIFLM QNDKLLNLEN ENSNEAFHEK RHELYEIVGD
SEYNKKNSLW KSPCHELILK DIFCDHCSSV YHMNVVTSLV EAEISGKPSF IWLCKNCNFK
FDNEFIELKI LSLLQETFDA YNAQDLVCNN CNAIKSFHRR PICKCGQIFT PRLEINNWMQ
TLEIVENLAS MLNMPLLTDV LTSMKTHLI
//
