ID W4Q5J4_9BACI Unreviewed; 758 AA.
AC W4Q5J4;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 34.
DE SubName: Full=Subtilase family domain protein {ECO:0000313|EMBL:GAE27230.1};
GN ORFNames=JCM9140_3358 {ECO:0000313|EMBL:GAE27230.1};
OS Halalkalibacter wakoensis JCM 9140.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Halalkalibacter.
OX NCBI_TaxID=1236970 {ECO:0000313|EMBL:GAE27230.1, ECO:0000313|Proteomes:UP000018890};
RN [1] {ECO:0000313|EMBL:GAE27230.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 9140 {ECO:0000313|EMBL:GAE27230.1};
RA Yuki M., Oshima K., Suda W., Oshida Y., Kitamura K., Iida T., Hattori M.,
RA Ohkuma M.;
RT "Draft Genome Sequences of Three Alkaliphilic Bacillus Strains, Bacillus
RT wakoensis JCM 9140T, Bacillus akibai JCM 9157T, and Bacillus
RT hemicellulosilyticus JCM 9152T.";
RL Genome Announc. 2:e01258-13(2014).
CC -!- SIMILARITY: Belongs to the peptidase S8 family.
CC {ECO:0000256|ARBA:ARBA00011073, ECO:0000256|PROSITE-ProRule:PRU01240,
CC ECO:0000256|RuleBase:RU003355}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAE27230.1}.
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DR EMBL; BAUT01000043; GAE27230.1; -; Genomic_DNA.
DR AlphaFoldDB; W4Q5J4; -.
DR STRING; 1236970.JCM9140_3358; -.
DR Proteomes; UP000018890; Unassembled WGS sequence.
DR GO; GO:0004252; F:serine-type endopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd02133; PA_C5a_like; 1.
DR CDD; cd07474; Peptidases_S8_subtilisin_Vpr-like; 1.
DR Gene3D; 3.50.30.30; -; 1.
DR Gene3D; 3.40.50.200; Peptidase S8/S53 domain; 1.
DR InterPro; IPR046450; PA_dom_sf.
DR InterPro; IPR003137; PA_domain.
DR InterPro; IPR000209; Peptidase_S8/S53_dom.
DR InterPro; IPR036852; Peptidase_S8/S53_dom_sf.
DR InterPro; IPR023827; Peptidase_S8_Asp-AS.
DR InterPro; IPR022398; Peptidase_S8_His-AS.
DR InterPro; IPR023828; Peptidase_S8_Ser-AS.
DR InterPro; IPR015500; Peptidase_S8_subtilisin-rel.
DR InterPro; IPR034213; S8_Vpr-like.
DR PANTHER; PTHR43806:SF59; CEREVISIN-RELATED; 1.
DR PANTHER; PTHR43806; PEPTIDASE S8; 1.
DR Pfam; PF02225; PA; 1.
DR Pfam; PF00082; Peptidase_S8; 1.
DR PRINTS; PR00723; SUBTILISIN.
DR SUPFAM; SSF52025; PA domain; 1.
DR SUPFAM; SSF52743; Subtilisin-like; 1.
DR PROSITE; PS51892; SUBTILASE; 1.
DR PROSITE; PS00136; SUBTILASE_ASP; 1.
DR PROSITE; PS00137; SUBTILASE_HIS; 1.
DR PROSITE; PS00138; SUBTILASE_SER; 1.
PE 3: Inferred from homology;
KW Cell wall {ECO:0000256|ARBA:ARBA00022512};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PROSITE-
KW ProRule:PRU01240};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Reference proteome {ECO:0000313|Proteomes:UP000018890};
KW Secreted {ECO:0000256|ARBA:ARBA00022512};
KW Serine protease {ECO:0000256|ARBA:ARBA00022825, ECO:0000256|PROSITE-
KW ProRule:PRU01240}; Signal {ECO:0000256|SAM:SignalP}.
FT SIGNAL 1..25
FT /evidence="ECO:0000256|SAM:SignalP"
FT CHAIN 26..758
FT /evidence="ECO:0000256|SAM:SignalP"
FT /id="PRO_5039359949"
FT DOMAIN 139..533
FT /note="Peptidase S8/S53"
FT /evidence="ECO:0000259|Pfam:PF00082"
FT DOMAIN 339..416
FT /note="PA"
FT /evidence="ECO:0000259|Pfam:PF02225"
FT ACT_SITE 148
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 188
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
FT ACT_SITE 481
FT /note="Charge relay system"
FT /evidence="ECO:0000256|PIRSR:PIRSR615500-1,
FT ECO:0000256|PROSITE-ProRule:PRU01240"
SQ SEQUENCE 758 AA; 82582 MW; B47D41CC0F62574F CRC64;
MDMNRVIACM LVLTMIVCNV GLASAEAAIY PEIPTVGKVD QSNEKVIVIA TANGNVEEAL
QQVNLAFPSS KVRKTFKRAL NGFSIELLES EVDMLRELQG IERVDQVVDY KANLDSSVPF
IGADEIRGKL DNNGEHLTGK GIKVAVIDTG IDYKHPDLEA NYKGGYDVVD YDHDPMETVA
SQGPPTLHGT HVAGIIAANG QVKGVAPEAD IYAYRALGPG GQGTTEQVIE AIEKAIEDEV
DVLNLSLGNT VNGPDWPTSI ALDKAVEEGI VAVTSNGNSG PNMWTVGSPG TSTKAISVGA
SAPPIKTPYV TIAGLDKEIS LYPIGGTLPW ALKRDFELVD VGYGLKEEWE EVDVNGKIAL
IERGMIPFSE KARYALDNGA KAVLIYNNLP GSFVGAVEQG MKIPVVAISK EDGDWLLEQL
EESKESQYAR TIYRNEEDFI APFSSRGPVT QTWEVKPDLV APGVSIDSTV PKGYLGLNGT
SMSAPHVAGA AALVKQAHPD WTPEQIKAAL MNTAKKLINE DGEPYYPYEQ GAGRLQVNRA
IDAKTLVYPG ALTFGKWSKD DPREKRKVTF TIENHDNHTR TFHVKPPFEL PDGLQWDVPF
STEIKAGEKK EVTVELDLFP AVLAEGIHHG DIVVEGGKDP ISIPYVFFIE EPNYPRAMAF
NFGHGDRPGE YRYEMYLPGG AEEMGIALYD PDTFQFVKFL DVKEDVGRGM VGKEWDDLDL
PDGTYKALIF AKKDGKEDTI ETNITIGALL PEGKKDED
//
