ID W4Q8N7_9BACI Unreviewed; 786 AA.
AC W4Q8N7;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 24-JAN-2024, entry version 46.
DE SubName: Full=Enoyl-CoA hydratase {ECO:0000313|EMBL:GAE28350.1};
GN ORFNames=JCM9140_4570 {ECO:0000313|EMBL:GAE28350.1};
OS Halalkalibacter wakoensis JCM 9140.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Halalkalibacter.
OX NCBI_TaxID=1236970 {ECO:0000313|EMBL:GAE28350.1, ECO:0000313|Proteomes:UP000018890};
RN [1] {ECO:0000313|EMBL:GAE28350.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 9140 {ECO:0000313|EMBL:GAE28350.1};
RA Yuki M., Oshima K., Suda W., Oshida Y., Kitamura K., Iida T., Hattori M.,
RA Ohkuma M.;
RT "Draft Genome Sequences of Three Alkaliphilic Bacillus Strains, Bacillus
RT wakoensis JCM 9140T, Bacillus akibai JCM 9157T, and Bacillus
RT hemicellulosilyticus JCM 9152T.";
RL Genome Announc. 2:e01258-13(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a (3S)-3-hydroxyacyl-CoA + NAD(+) = a 3-oxoacyl-CoA + H(+) +
CC NADH; Xref=Rhea:RHEA:22432, ChEBI:CHEBI:15378, ChEBI:CHEBI:57318,
CC ChEBI:CHEBI:57540, ChEBI:CHEBI:57945, ChEBI:CHEBI:90726; EC=1.1.1.35;
CC Evidence={ECO:0000256|ARBA:ARBA00023693};
CC -!- PATHWAY: Lipid metabolism; fatty acid beta-oxidation.
CC {ECO:0000256|ARBA:ARBA00005005}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAE28350.1}.
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DR EMBL; BAUT01000098; GAE28350.1; -; Genomic_DNA.
DR RefSeq; WP_034750983.1; NZ_BAZN01000098.1.
DR AlphaFoldDB; W4Q8N7; -.
DR STRING; 1236970.JCM9140_4570; -.
DR OrthoDB; 9771883at2; -.
DR UniPathway; UPA00659; -.
DR Proteomes; UP000018890; Unassembled WGS sequence.
DR GO; GO:0003857; F:3-hydroxyacyl-CoA dehydrogenase activity; IEA:UniProtKB-EC.
DR GO; GO:0070403; F:NAD+ binding; IEA:InterPro.
DR GO; GO:0006635; P:fatty acid beta-oxidation; IEA:UniProtKB-UniPathway.
DR CDD; cd06558; crotonase-like; 1.
DR Gene3D; 1.10.1040.50; -; 1.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR006176; 3-OHacyl-CoA_DH_NAD-bd.
DR InterPro; IPR006108; 3HC_DH_C.
DR InterPro; IPR008927; 6-PGluconate_DH-like_C_sf.
DR InterPro; IPR029045; ClpP/crotonase-like_dom_sf.
DR InterPro; IPR001753; Enoyl-CoA_hydra/iso.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR PANTHER; PTHR48075; 3-HYDROXYACYL-COA DEHYDROGENASE FAMILY PROTEIN; 1.
DR PANTHER; PTHR48075:SF7; 3-HYDROXYACYL-COA DEHYDROGENASE-RELATED; 1.
DR Pfam; PF00725; 3HCDH; 1.
DR Pfam; PF02737; 3HCDH_N; 1.
DR Pfam; PF00378; ECH_1; 1.
DR SUPFAM; SSF48179; 6-phosphogluconate dehydrogenase C-terminal domain-like; 2.
DR SUPFAM; SSF52096; ClpP/crotonase; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
PE 4: Predicted;
KW Fatty acid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00022832};
KW NAD {ECO:0000256|ARBA:ARBA00023027};
KW Reference proteome {ECO:0000313|Proteomes:UP000018890}.
FT DOMAIN 7..205
FT /note="3-hydroxyacyl-CoA dehydrogenase NAD binding"
FT /evidence="ECO:0000259|Pfam:PF02737"
FT DOMAIN 208..305
FT /note="3-hydroxyacyl-CoA dehydrogenase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF00725"
SQ SEQUENCE 786 AA; 86299 MW; 210DC4F454824F2C CRC64;
MTTQIRKVAV IGSGVMGSGI AAHLANVGIP SLLLDIVPTK LTADEEAKGL TLEDYSVRNR
LSTLAIQKLK KQKPAPLSSK TKASYIEAGN LEDDLERLKE VDWIIEVIVE NLAIKKQLFS
RIDQVRKPGT IVSSNTSGIS IQAMSEGLSE DFQKHFLGTH FFNPPRYLKL LEVIPTEQTS
QEVLSFMKAF AEEVLGKGVV ECKDTPNFIA NRIGTYGLLV TVNEMIQGGY SVGEVDSVTG
PLLGRPKSAT FRTLDVVGLD TFLHVAKNVY DVADGVEKET FDPPSFMKEM AEKGWLGSKS
GQGFYLKQGK EILEVDPQTL EYQERKKLKA PSIEQAKQAK KLPDKLKTMA YANDRAGQLV
WNLLKPALLY SAEKAYEIAN DISAIDDAMK WGFGWELGPF ETWDALGVAK SVEKMEAEGE
TVPSWVKDML ESGQESFYEA NTFYHNGTLE PKSIHEKIVN LKSLKPTNVV FKNSGASLID
IGEDVALLEF HSPNNSIGAD VIQMINKSIK EVETNYKGLV INNQGKNFCV GANLMLILME
AQDDNFFEID LVVRQFQQAM ANIRYSEKPV VTAPFGMTLG GGTEICLPSA SIQASLETYM
GLVEVGVGLV PGGGGNKELY LRNLQNSDGK DLQAVANKTF ETIAMAKVST SAQEAVEHGF
LGARDGIVIN SDFLIDRAKS EVLHLAEKGY RPPERAKIPV VGETGYATML LGAQTMKFGG
MISDHDLKIA KKLAFLLAGG RVAKGTLVDE QYLLDLEREA FLSLVAEPKS QQRMQYMLTK
GKPLRN
//
