ID W4Q8Z8_9BACI Unreviewed; 595 AA.
AC W4Q8Z8;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Oligoendopeptidase F {ECO:0000256|RuleBase:RU368091};
DE EC=3.4.24.- {ECO:0000256|RuleBase:RU368091};
GN ORFNames=JCM9140_4697 {ECO:0000313|EMBL:GAE28460.1};
OS Halalkalibacter wakoensis JCM 9140.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Halalkalibacter.
OX NCBI_TaxID=1236970 {ECO:0000313|EMBL:GAE28460.1, ECO:0000313|Proteomes:UP000018890};
RN [1] {ECO:0000313|EMBL:GAE28460.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 9140 {ECO:0000313|EMBL:GAE28460.1};
RA Yuki M., Oshima K., Suda W., Oshida Y., Kitamura K., Iida T., Hattori M.,
RA Ohkuma M.;
RT "Draft Genome Sequences of Three Alkaliphilic Bacillus Strains, Bacillus
RT wakoensis JCM 9140T, Bacillus akibai JCM 9157T, and Bacillus
RT hemicellulosilyticus JCM 9152T.";
RL Genome Announc. 2:e01258-13(2014).
CC -!- FUNCTION: Has oligopeptidase activity and degrades a variety of small
CC bioactive peptides. {ECO:0000256|RuleBase:RU368091}.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|RuleBase:RU368091};
CC Note=Binds 1 zinc ion. {ECO:0000256|RuleBase:RU368091};
CC -!- SIMILARITY: Belongs to the peptidase M3B family.
CC {ECO:0000256|RuleBase:RU368091}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAE28460.1}.
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DR EMBL; BAUT01000109; GAE28460.1; -; Genomic_DNA.
DR RefSeq; WP_034751269.1; NZ_BAZN01000109.1.
DR AlphaFoldDB; W4Q8Z8; -.
DR STRING; 1236970.JCM9140_4697; -.
DR OrthoDB; 9766487at2; -.
DR Proteomes; UP000018890; Unassembled WGS sequence.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004222; F:metalloendopeptidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR CDD; cd09608; M3B_PepF; 1.
DR Gene3D; 1.10.1370.20; Oligoendopeptidase f, C-terminal domain; 1.
DR Gene3D; 1.20.140.70; Oligopeptidase f, N-terminal domain; 1.
DR Gene3D; 1.10.287.830; putative peptidase helix hairpin domain like; 1.
DR InterPro; IPR013647; OligopepF_N_dom.
DR InterPro; IPR042088; OligoPept_F_C.
DR InterPro; IPR045090; Pept_M3A_M3B.
DR InterPro; IPR001567; Pept_M3A_M3B_dom.
DR InterPro; IPR004438; Peptidase_M3B.
DR NCBIfam; TIGR00181; pepF; 1.
DR PANTHER; PTHR11804:SF79; MITOCHONDRIAL INTERMEDIATE PEPTIDASE; 1.
DR PANTHER; PTHR11804; PROTEASE M3 THIMET OLIGOPEPTIDASE-RELATED; 1.
DR Pfam; PF01432; Peptidase_M3; 1.
DR Pfam; PF08439; Peptidase_M3_N; 1.
DR SUPFAM; SSF55486; Metalloproteases ('zincins'), catalytic domain; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|RuleBase:RU368091};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU368091};
KW Metalloprotease {ECO:0000256|ARBA:ARBA00023049,
KW ECO:0000256|RuleBase:RU368091};
KW Protease {ECO:0000256|ARBA:ARBA00022670, ECO:0000256|RuleBase:RU368091};
KW Reference proteome {ECO:0000313|Proteomes:UP000018890};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU368091}.
FT DOMAIN 114..182
FT /note="Oligopeptidase F N-terminal"
FT /evidence="ECO:0000259|Pfam:PF08439"
FT DOMAIN 203..582
FT /note="Peptidase M3A/M3B catalytic"
FT /evidence="ECO:0000259|Pfam:PF01432"
SQ SEQUENCE 595 AA; 68665 MW; 7A4B935D84E4F30D CRC64;
MPNYKSREEV PLEEKWDLHD IYDSLTTWES DLKSVAERTE QLKTYDGNIT DAKSLYDYLV
LSEETGYTYK KVYVYAMLQA DLDTRDSKPQ ALLDRAKSLG VKLSSATSFF MPFLLSLDEK
TLKSYIDQIE GLKYFEEDLM ESYRYKTHVL TKEKEEVLSD LGEALSSPSN TFGMINNADI
KFGKVTTKDG EKVELTRGMY SKLIEDDNRD VRREAYKAYY QPYVQLNNTI ASTLSAAIKN
NVTLAKMRKY PSALEKSLFS DMVPKDVYDN LIKVTKDHIT PLHKYQNIRK ETLGLDELRQ
YDLNVPLVKD VKEEITFDEA YQTMLDSLAP LGEEYVETLR SFKEKRYFDV RETPGKRSGA
YNLGLYGVHP FVLLNHQDDL DSLFTLVHEC GHAMHSWYSS NHQPQITAGY SIFVAEVAST
VNEVLLIRYL LKTTKDEKMK KHLLNHFIDS FRGTMFTQVM FAEFEKTTHE KAENGGPLNA
EVFNDVYEKI FREFNGNEMV FDDEVKYGWS RIPHFYRPFY VYKYATGYAA AIHIADQLLE
GKSETLEKYM TFLKSGSSDY PLELLKAAGV DLTKPEPIRS ALTIFSELVD EFASL
//
