ID W4QD17_9BACI Unreviewed; 728 AA.
AC W4QD17;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 35.
DE RecName: Full=Alpha-galactosidase {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
DE EC=3.2.1.22 {ECO:0000256|ARBA:ARBA00012755, ECO:0000256|PIRNR:PIRNR005536};
GN ORFNames=JCM9152_1325 {ECO:0000313|EMBL:GAE29936.1};
OS Halalkalibacter hemicellulosilyticusJCM 9152.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Halalkalibacter.
OX NCBI_TaxID=1236971 {ECO:0000313|EMBL:GAE29936.1, ECO:0000313|Proteomes:UP000018895};
RN [1] {ECO:0000313|EMBL:GAE29936.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 9152 {ECO:0000313|EMBL:GAE29936.1};
RA Yuki M., Oshima K., Suda W., Oshida Y., Kitamura K., Iida T., Hattori M.,
RA Ohkuma M.;
RT "Draft Genome Sequences of Three Alkaliphilic Bacillus Strains, Bacillus
RT wakoensis JCM 9140T, Bacillus akibai JCM 9157T, and Bacillus
RT hemicellulosilyticus JCM 9152T.";
RL Genome Announc. 2:e01258-13(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of terminal, non-reducing alpha-D-galactose
CC residues in alpha-D-galactosides, including galactose
CC oligosaccharides, galactomannans and galactolipids.; EC=3.2.1.22;
CC Evidence={ECO:0000256|ARBA:ARBA00001255,
CC ECO:0000256|PIRNR:PIRNR005536};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 36 family.
CC {ECO:0000256|ARBA:ARBA00006202}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAE29936.1}.
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DR EMBL; BAUU01000008; GAE29936.1; -; Genomic_DNA.
DR RefSeq; WP_035342065.1; NZ_BAZO01000008.1.
DR AlphaFoldDB; W4QD17; -.
DR STRING; 1236971.JCM9152_1325; -.
DR OrthoDB; 9758822at2; -.
DR Proteomes; UP000018895; Unassembled WGS sequence.
DR GO; GO:0004557; F:alpha-galactosidase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0016052; P:carbohydrate catabolic process; IEA:InterPro.
DR CDD; cd14791; GH36; 1.
DR Gene3D; 3.20.20.70; Aldolase class I; 1.
DR Gene3D; 2.70.98.60; alpha-galactosidase from lactobacil brevis; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR013785; Aldolase_TIM.
DR InterPro; IPR038417; Alpga-gal_N_sf.
DR InterPro; IPR000111; Glyco_hydro_27/36_CS.
DR InterPro; IPR002252; Glyco_hydro_36.
DR InterPro; IPR031705; Glyco_hydro_36_C.
DR InterPro; IPR031704; Glyco_hydro_36_N.
DR InterPro; IPR013780; Glyco_hydro_b.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR PANTHER; PTHR43053:SF3; ALPHA-GALACTOSIDASE C-RELATED; 1.
DR PANTHER; PTHR43053; GLYCOSIDASE FAMILY 31; 1.
DR Pfam; PF16874; Glyco_hydro_36C; 1.
DR Pfam; PF16875; Glyco_hydro_36N; 1.
DR Pfam; PF02065; Melibiase; 1.
DR PIRSF; PIRSF005536; Agal; 1.
DR PRINTS; PR00743; GLHYDRLASE36.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR PROSITE; PS00512; ALPHA_GALACTOSIDASE; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|PIRNR:PIRNR005536};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801, ECO:0000256|PIRNR:PIRNR005536};
KW Reference proteome {ECO:0000313|Proteomes:UP000018895}.
FT DOMAIN 29..285
FT /note="Glycosyl hydrolase family 36 N-terminal"
FT /evidence="ECO:0000259|Pfam:PF16875"
FT DOMAIN 649..725
FT /note="Glycosyl hydrolase family 36 C-terminal"
FT /evidence="ECO:0000259|Pfam:PF16874"
FT ACT_SITE 478
FT /note="Nucleophile"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
FT ACT_SITE 548
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR005536-1"
SQ SEQUENCE 728 AA; 84240 MW; 4391CF4845845CF6 CRC64;
MGITFVEQTR TFHLQANDMS YVFQVLDGGY VAHLYWGKKV NRYHHSNAIR YADRGFSGNP
IASDRTFSLD TLPQEYPSFG HTDYRLPAVH IQLENGSTVM DLRYHSHRIE KGKPQLNGLP
ATYVENEDEA ETLEITLLDS LTQLRVVLTY TVFEKWNAMT RSVRFENNGE ESLKIEKAAS
VNVDFRSANF EMLRLPGAHC RERHIEQSPL HTGIQSVESR RGASSHQHNP FIALVDENTT
EDTGEVFAFN LVYSGNFLAT AEVDQFSQTR VTMGINPFDF SWLLQPGENF QTPEVVMVYS
SNGLTEMSHT FHRLYQQRLC RGVFRDLERP ILINNWEATY FDFHEDKIYD IAKSGKELGI
ELFVLDDGWF GKRDDDNSSL GDWFVDEKKL PNGLEELARK MTKLDMQFGL WFEPEMVSED
SELYRQHPDW CIHVPNRRRS KSRNQLVLDY SRHDVCDEMI KRISTILQQA PITYVKWDMN
RHMTEVGSAQ LSKNRQRETG HRYMLGLYRV LEELTIAFPH VLFESCSGGG GRFDPGMLYY
MPQTWTSDNT DAISRLKIQY GTSLVYPIRS MGAHVSAVPN HQVQRETPIE VRGHVAMSGN
FGYELDVTTL SSEEHQVIKE QVAYYKTIRH IVQFGHMYRL VNPFKQQDAA WMFVTDDQSE
AVFSYVKVLG EANEPFKRIK LKGLIKTKKY HIIELSESYY GDELMNIGVN IPQLTGDFQS
VMWRLIEE
//
