ID W4QGL7_9BACI Unreviewed; 637 AA.
AC W4QGL7;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 31.
DE RecName: Full=pullulanase {ECO:0000256|ARBA:ARBA00024062};
DE EC=3.2.1.41 {ECO:0000256|ARBA:ARBA00024062};
DE AltName: Full=Alpha-dextrin endo-1,6-alpha-glucosidase {ECO:0000256|ARBA:ARBA00029618};
DE AltName: Full=Pullulan 6-glucanohydrolase {ECO:0000256|ARBA:ARBA00031076};
GN ORFNames=JCM9152_1862 {ECO:0000313|EMBL:GAE30454.1};
OS Halalkalibacter hemicellulosilyticusJCM 9152.
OC Bacteria; Bacillota; Bacilli; Bacillales; Bacillaceae; Halalkalibacter.
OX NCBI_TaxID=1236971 {ECO:0000313|EMBL:GAE30454.1, ECO:0000313|Proteomes:UP000018895};
RN [1] {ECO:0000313|EMBL:GAE30454.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 9152 {ECO:0000313|EMBL:GAE30454.1};
RA Yuki M., Oshima K., Suda W., Oshida Y., Kitamura K., Iida T., Hattori M.,
RA Ohkuma M.;
RT "Draft Genome Sequences of Three Alkaliphilic Bacillus Strains, Bacillus
RT wakoensis JCM 9140T, Bacillus akibai JCM 9157T, and Bacillus
RT hemicellulosilyticus JCM 9152T.";
RL Genome Announc. 2:e01258-13(2014).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Hydrolysis of (1->6)-alpha-D-glucosidic linkages in pullulan,
CC amylopectin and glycogen, and in the alpha- and beta-limit dextrins
CC of amylopectin and glycogen.; EC=3.2.1.41;
CC Evidence={ECO:0000256|ARBA:ARBA00023965};
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAE30454.1}.
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DR EMBL; BAUU01000011; GAE30454.1; -; Genomic_DNA.
DR RefSeq; WP_052015770.1; NZ_BAZO01000011.1.
DR AlphaFoldDB; W4QGL7; -.
DR STRING; 1236971.JCM9152_1862; -.
DR OrthoDB; 9761875at2; -.
DR Proteomes; UP000018895; Unassembled WGS sequence.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR CDD; cd11341; AmyAc_Pullulanase_LD-like; 1.
DR CDD; cd02860; E_set_Pullulanase; 1.
DR Gene3D; 3.20.20.80; Glycosidases; 1.
DR Gene3D; 2.60.40.10; Immunoglobulins; 1.
DR InterPro; IPR006047; Glyco_hydro_13_cat_dom.
DR InterPro; IPR004193; Glyco_hydro_13_N.
DR InterPro; IPR017853; Glycoside_hydrolase_SF.
DR InterPro; IPR013783; Ig-like_fold.
DR InterPro; IPR014756; Ig_E-set.
DR InterPro; IPR011840; PulA_typeI.
DR NCBIfam; TIGR02104; pulA_typeI; 1.
DR PANTHER; PTHR43002; GLYCOGEN DEBRANCHING ENZYME; 1.
DR PANTHER; PTHR43002:SF11; PULLULANASE 1, CHLOROPLASTIC; 1.
DR Pfam; PF00128; Alpha-amylase; 1.
DR Pfam; PF02922; CBM_48; 1.
DR SMART; SM00642; Aamy; 1.
DR SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR SUPFAM; SSF81296; E set domains; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000018895}.
FT DOMAIN 239..605
FT /note="Glycosyl hydrolase family 13 catalytic"
FT /evidence="ECO:0000259|SMART:SM00642"
SQ SEQUENCE 637 AA; 73469 MW; 34335CE4A778EEAA CRC64;
MSSTIAWLDD VHDLRLTSEG LEDLSFNTPL LIKGKKQNFS VHLVGIEGEN TAKFHVEEPL
PLGEELSLHI EGKRFRIYPR NIIRTNWFEQ NYTNMDIELG AICTKDETKF RLWAPTATSV
KLYINNNIMS LKKYEKGIWQ VEIQGDLHGM TYEYELFVNG EVVRAIDPYT KALLANSEKG
VVVDLKRLKG FTKIERNYHE ISALNDAIIY EIHVRDATIS EKSGVREKGK FIGLAETKTT
TPNGFSTGIS YIKDLGITHV QLLPINDFAR VNELEPEESY NWGYDPLFFQ VPEGSYSTDV
LNPLTRMEEC KEMISAFHKE GIGVILDVVF NHVYIREESS FEKIVPGYFF RYDENGEVSN
GTGVGNDFAT ERIMARKFIV DSIAYWLQEY QVDGFRFDLM GSMDVETIKA IEKRCQKEER
PIILLGEGWD LPTALRYELK TTPDKAKQLR KVGFFNDLFR DSLKGQLFSD RDLGYVNGGG
CFVERLPHLI TGSVLDEYGI PFVSEPNQSI NYVECHDNHT LWDRLKNTNP TDNEETRKRM
HQLATGITLL SQGIPFLHAG QEWFRTKHGD GNSYISGDTV NKLDWEKREV DAENIEFIRK
LITIRKKYRV FRLRSKDQIR KQIHFLKVQP PILGIFY
//
