ID W4S8U3_9XANT Unreviewed; 538 AA.
AC W4S8U3;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 24-JAN-2024, entry version 29.
DE RecName: Full=malate synthase {ECO:0000256|ARBA:ARBA00012636};
DE EC=2.3.3.9 {ECO:0000256|ARBA:ARBA00012636};
GN ORFNames=XPU_4314 {ECO:0000313|EMBL:GAE52782.1};
OS Xanthomonas arboricola pv. pruni str. MAFF 311562.
OC Bacteria; Pseudomonadota; Gammaproteobacteria; Xanthomonadales;
OC Xanthomonadaceae; Xanthomonas.
OX NCBI_TaxID=1414836 {ECO:0000313|EMBL:GAE52782.1, ECO:0000313|Proteomes:UP000019143};
RN [1] {ECO:0000313|EMBL:GAE52782.1, ECO:0000313|Proteomes:UP000019143}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=MAFF 311562 {ECO:0000313|Proteomes:UP000019143};
RA Fujikawa T., Nakazono-Nagaoka E.;
RT "Genome sequence and analysis of Xanthomonas arboricola pv. pruni.";
RL Submitted (JAN-2014) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA + H(+);
CC Xref=Rhea:RHEA:18181, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:15589, ChEBI:CHEBI:36655, ChEBI:CHEBI:57287,
CC ChEBI:CHEBI:57288; EC=2.3.3.9;
CC Evidence={ECO:0000256|ARBA:ARBA00001699};
CC -!- SIMILARITY: Belongs to the malate synthase family.
CC {ECO:0000256|ARBA:ARBA00006394}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAE52782.1}.
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DR EMBL; BAVB01000379; GAE52782.1; -; Genomic_DNA.
DR AlphaFoldDB; W4S8U3; -.
DR Proteomes; UP000019143; Unassembled WGS sequence.
DR GO; GO:0004474; F:malate synthase activity; IEA:UniProtKB-EC.
DR GO; GO:0006097; P:glyoxylate cycle; IEA:UniProtKB-KW.
DR GO; GO:0006099; P:tricarboxylic acid cycle; IEA:UniProtKB-KW.
DR CDD; cd00727; malate_synt_A; 1.
DR Gene3D; 3.20.20.360; Malate synthase, domain 3; 1.
DR Gene3D; 1.20.1220.12; Malate synthase, domain III; 1.
DR InterPro; IPR044856; Malate_synth_C_sf.
DR InterPro; IPR011076; Malate_synth_sf.
DR InterPro; IPR006252; Malate_synthA.
DR InterPro; IPR001465; Malate_synthase_TIM.
DR InterPro; IPR048355; MS_C.
DR InterPro; IPR048356; MS_N.
DR InterPro; IPR046363; MS_N_TIM-barrel_dom.
DR NCBIfam; TIGR01344; malate_syn_A; 1.
DR PANTHER; PTHR42902; MALATE SYNTHASE; 1.
DR PANTHER; PTHR42902:SF1; MALATE SYNTHASE 1-RELATED; 1.
DR Pfam; PF20659; MS_C; 1.
DR Pfam; PF20656; MS_N; 1.
DR Pfam; PF01274; MS_TIM-barrel; 1.
DR PIRSF; PIRSF001363; Malate_synth; 1.
DR SUPFAM; SSF51645; Malate synthase G; 1.
PE 3: Inferred from homology;
KW Acyltransferase {ECO:0000313|EMBL:GAE52782.1};
KW Glyoxylate bypass {ECO:0000256|ARBA:ARBA00022435};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000313|EMBL:GAE52782.1};
KW Tricarboxylic acid cycle {ECO:0000256|ARBA:ARBA00022532}.
FT DOMAIN 25..76
FT /note="Malate synthase N-terminal"
FT /evidence="ECO:0000259|Pfam:PF20656"
FT DOMAIN 170..413
FT /note="Malate synthase TIM barrel"
FT /evidence="ECO:0000259|Pfam:PF01274"
FT DOMAIN 420..536
FT /note="Malate synthase C-terminal"
FT /evidence="ECO:0000259|Pfam:PF20659"
FT ACT_SITE 174
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
FT ACT_SITE 454
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR001363-1"
SQ SEQUENCE 538 AA; 59083 MW; B929F534BEA9864A CRC64;
MSATAFAPRP TERATPGLSL TTQVAGQAEL LPAAALALLV SLHRAIEPGR QQCLAQRRAR
QAEFDAGQLP DFRPDTRAIR DGDWRVAALP SALQDRRVEI TGPTDPKMVI NALNSGAKVF
MADFEDSTAP TWRNLLAGQR TLAAAVRGEL SFDAPNGKHY ALRPEAERAV LIVRPRGWHL
DEKHVLIDGQ PLAGGLFDAA VFAFHNGRAL LAKDRGPYLY LPKLQSMEEA ALWETALAHI
EAMLGLPHGQ IKVTVLIETL PAVFEMDEIL HALRERIVGL NCGRWDYIFS YLKTFRAHRD
RVLPERGQVT MTQPFLKAYS ELLIKTCHRR GAHAMGGMAA QIPINHDEAA NEQAMARVRA
DKLREVSAGH DGTWVAHPAL IPVAMKIFDE HMPTAHQQHV LRNDVQVTRD LLIAPSPGTV
TRAGFEGNVE VCVRYLAAWL DGNGCVPIHN LMEDAATAEI SRAQLWQWLH HGQHLDDGTA
IDQPLLQATL RALPARLGAT DALPGAARID DAIALLDTLS RADALADFLT VPAYRLID
//
