ID W4V9V6_9FIRM Unreviewed; 728 AA.
AC W4V9V6;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 27-MAR-2024, entry version 41.
DE SubName: Full=Beta-galactosidase {ECO:0000313|EMBL:GAE90210.1};
GN ORFNames=JCM21531_3801 {ECO:0000313|EMBL:GAE90210.1};
OS Acetivibrio straminisolvens JCM 21531.
OC Bacteria; Bacillota; Clostridia; Eubacteriales; Oscillospiraceae;
OC Acetivibrio.
OX NCBI_TaxID=1294263 {ECO:0000313|EMBL:GAE90210.1, ECO:0000313|Proteomes:UP000019109};
RN [1] {ECO:0000313|EMBL:GAE90210.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=JCM 21531 {ECO:0000313|EMBL:GAE90210.1};
RA Yuki M., Oshima K., Suda W., Sakamoto M., Kitamura K., Iida T., Hattori M.,
RA Ohkuma M.;
RT "Draft Genome Sequence of Clostridium straminisolvens Strain JCM 21531T,
RT Isolated from a Cellulose-Degrading Bacterial Community.";
RL Genome Announc. 2:e00110-14(2014).
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 43 family.
CC {ECO:0000256|ARBA:ARBA00009865}.
CC -!- CAUTION: The sequence shown here is derived from an EMBL/GenBank/DDBJ
CC whole genome shotgun (WGS) entry which is preliminary data.
CC {ECO:0000313|EMBL:GAE90210.1}.
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DR EMBL; BAVR01000059; GAE90210.1; -; Genomic_DNA.
DR RefSeq; WP_038290784.1; NZ_BAVR01000059.1.
DR AlphaFoldDB; W4V9V6; -.
DR STRING; 1294263.JCM21531_3801; -.
DR OrthoDB; 9801455at2; -.
DR Proteomes; UP000019109; Unassembled WGS sequence.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0004553; F:hydrolase activity, hydrolyzing O-glycosyl compounds; IEA:InterPro.
DR GO; GO:0000272; P:polysaccharide catabolic process; IEA:InterPro.
DR CDD; cd04084; CBM6_xylanase-like; 1.
DR CDD; cd14256; Dockerin_I; 1.
DR CDD; cd09001; GH43_FsAxh1-like; 1.
DR Gene3D; 2.60.120.200; -; 1.
DR Gene3D; 1.10.1330.10; Dockerin domain; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR InterPro; IPR006584; Cellulose-bd_IV.
DR InterPro; IPR005084; CMB_fam6.
DR InterPro; IPR013320; ConA-like_dom_sf.
DR InterPro; IPR002105; Dockerin_1_rpt.
DR InterPro; IPR016134; Dockerin_dom.
DR InterPro; IPR036439; Dockerin_dom_sf.
DR InterPro; IPR018247; EF_Hand_1_Ca_BS.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR041542; GH43_C2.
DR InterPro; IPR006710; Glyco_hydro_43.
DR InterPro; IPR023296; Glyco_hydro_beta-prop_sf.
DR PANTHER; PTHR42812; BETA-XYLOSIDASE; 1.
DR PANTHER; PTHR42812:SF13; HYDROLASE, PUTATIVE (AFU_ORTHOLOGUE AFUA_2G00930)-RELATED; 1.
DR Pfam; PF03422; CBM_6; 1.
DR Pfam; PF00404; Dockerin_1; 1.
DR Pfam; PF17851; GH43_C2; 1.
DR Pfam; PF04616; Glyco_hydro_43; 1.
DR SMART; SM00606; CBD_IV; 1.
DR SUPFAM; SSF75005; Arabinanase/levansucrase/invertase; 1.
DR SUPFAM; SSF49899; Concanavalin A-like lectins/glucanases; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF63446; Type I dockerin domain; 1.
DR PROSITE; PS51175; CBM6; 1.
DR PROSITE; PS51766; DOCKERIN; 1.
DR PROSITE; PS00018; EF_HAND_1; 1.
PE 3: Inferred from homology;
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295};
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Reference proteome {ECO:0000313|Proteomes:UP000019109}.
FT DOMAIN 535..658
FT /note="CBM6"
FT /evidence="ECO:0000259|PROSITE:PS51175"
FT DOMAIN 662..728
FT /note="Dockerin"
FT /evidence="ECO:0000259|PROSITE:PS51766"
FT ACT_SITE 56
FT /note="Proton acceptor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT ACT_SITE 213
FT /note="Proton donor"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-1"
FT SITE 164
FT /note="Important for catalytic activity, responsible for
FT pKa modulation of the active site Glu and correct
FT orientation of both the proton donor and substrate"
FT /evidence="ECO:0000256|PIRSR:PIRSR606710-2"
SQ SEQUENCE 728 AA; 80489 MW; 02F6690DB4DD0B10 CRC64;
MLFAKKPKRS LGLKKAIVIL LVLCMSLLQS IFVYGWQSDN GDGTFNNPPL YADYPDPSII
RVGNYFYMAS STFVGVPGLV ILKSEDLVNW EIAGHCISSF TGDSKYNLEG GTKYGNGCYA
PSIAYKNGTF YVAVTPNGER TRIYYAKDVA GPWNYNTLGG SYFDPCLFID DDGSAYLAYG
GAWENSIKMI QLNSNLSATV GSSRVILSYK SVEGTHLSKV NGRYYLFNAV PAQRLVCSRA
NNVWGPYGET ITLCTAGKGG HQGGIVDLPD GTYWGYLHQD DGAIGRPTRI CPITWQNGWP
MFGRPGYIGQ VESKYTKPIQ NKPIKVPAAS DEFNSDSLGL QWMWNHNPDN SKWSLTGTSL
RLKATTAKDF WTARNSLTQK GQGPISTGTI KIDCSGMQPG DICGLGMLGD PRGYIAVTRD
PKRIIMTEEE VVKATVNNIT SNILYFRLEM NFMTKQARFF WKDDNRDWQQ LGPAITMGFD
WQYGTFQGEQ YAILNFNPNG STGYMDVDWF RLNDIPGDNQ TPQPTPTPIP RQAFTKIEAE
SYNNQLGIQT EKCSEGGENI GFIENGDYAV YKNIDFGNGA ASFEARVASA TNGGKIELRI
DSINGPLIGT CPVAGTGDWQ TWNTVSCDVS EVKGNHDLYL VFTGGSGYLF NVNWFTFIEA
NNDEHLGDLN GDGKVNSTDL QVLKKHLLRI TSLTGKELSN ADVTKDGKVD SSDYTLLKRY
ILKFITDF
//
