ID W5C5B1_WHEAT Unreviewed; 352 AA.
AC W5C5B1;
DT 19-MAR-2014, integrated into UniProtKB/TrEMBL.
DT 19-MAR-2014, sequence version 1.
DT 24-JAN-2024, entry version 61.
DE RecName: Full=rRNA adenine N(6)-methyltransferase {ECO:0000256|RuleBase:RU362106};
DE EC=2.1.1.- {ECO:0000256|RuleBase:RU362106};
GN ORFNames=CAMPLR22A2D_LOCUS2167 {ECO:0000313|EMBL:SPT17557.1},
GN CFC21_029367 {ECO:0000313|EMBL:KAF7015541.1};
OS Triticum aestivum (Wheat).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; Liliopsida; Poales; Poaceae; BOP clade;
OC Pooideae; Triticodae; Triticeae; Triticinae; Triticum.
OX NCBI_TaxID=4565 {ECO:0000313|EnsemblPlants:TraesCS2D02G231300.1};
RN [1] {ECO:0000313|EMBL:KAF7015541.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7015541.1};
RX PubMed=29069494;
RA Zimin A.V., Puiu D., Hall R., Kingan S., Clavijo B.J., Salzberg S.L.;
RT "The first near-complete assembly of the hexaploid bread wheat genome,
RT Triticum aestivum.";
RL Gigascience 6:1-7(2017).
RN [2] {ECO:0000313|EnsemblPlants:TraesCS2D02G231300.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Chinese Spring
RC {ECO:0000313|EnsemblPlants:TraesCS2D02G231300.1};
RX PubMed=30115783; DOI=10.1126/science.aar7191;
RG International wheat genome sequencing consortium (IWGSC);
RT "Shifting the limits in wheat research and breeding using a fully annotated
RT reference genome.";
RL Science 361:EAAR7191-EAAR7191(2018).
RN [3] {ECO:0000313|EMBL:SPT17557.1, ECO:0000313|Proteomes:UP000280104}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Thind KAUR A.;
RL Submitted (MAY-2018) to the EMBL/GenBank/DDBJ databases.
RN [4] {ECO:0000313|EnsemblPlants:TraesCS2D02G231300.1}
RP IDENTIFICATION.
RG EnsemblPlants;
RL Submitted (OCT-2018) to UniProtKB.
RN [5] {ECO:0000313|EMBL:KAF7015541.1}
RP NUCLEOTIDE SEQUENCE.
RC TISSUE=Leaf {ECO:0000313|EMBL:KAF7015541.1};
RA Zimin A.V., Puiu D., Shumante A., Alonge M., Salzberg S.L.;
RT "The second near-complete assembly of the hexaploid bread wheat (Triticum
RT aestivum) genome.";
RL Submitted (MAR-2020) to the EMBL/GenBank/DDBJ databases.
CC -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC superfamily. rRNA adenine N(6)-methyltransferase family.
CC {ECO:0000256|PROSITE-ProRule:PRU01026, ECO:0000256|RuleBase:RU362106}.
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DR EMBL; CM022216; KAF7015541.1; -; Genomic_DNA.
DR EMBL; LS480641; SPT17557.1; -; Genomic_DNA.
DR SMR; W5C5B1; -.
DR STRING; 4565.W5C5B1; -.
DR PaxDb; 4565-Traes_2DS_5C8A4D416-1; -.
DR EnsemblPlants; TraesCS2D02G231300.1; TraesCS2D02G231300.1; TraesCS2D02G231300.
DR Gramene; TraesCAD_scaffold_015599_01G000200.1; TraesCAD_scaffold_015599_01G000200.1; TraesCAD_scaffold_015599_01G000200.
DR Gramene; TraesCLE_scaffold_114533_01G000200.1; TraesCLE_scaffold_114533_01G000200.1; TraesCLE_scaffold_114533_01G000200.
DR Gramene; TraesCS2D02G231300.1; TraesCS2D02G231300.1; TraesCS2D02G231300.
DR Gramene; TraesCS2D03G0489000.1; TraesCS2D03G0489000.1.CDS; TraesCS2D03G0489000.
DR Gramene; TraesKAR2D01G0150530.1; cds.TraesKAR2D01G0150530.1; TraesKAR2D01G0150530.
DR Gramene; TraesPAR_scaffold_081620_01G000200.1; TraesPAR_scaffold_081620_01G000200.1; TraesPAR_scaffold_081620_01G000200.
DR Gramene; TraesRN2D0100521400.1; TraesRN2D0100521400.1; TraesRN2D0100521400.
DR Gramene; TraesROB_scaffold_017435_01G000300.1; TraesROB_scaffold_017435_01G000300.1; TraesROB_scaffold_017435_01G000300.
DR Gramene; TraesWEE_scaffold_015146_01G000300.1; TraesWEE_scaffold_015146_01G000300.1; TraesWEE_scaffold_015146_01G000300.
DR eggNOG; KOG0820; Eukaryota.
DR HOGENOM; CLU_041220_2_0_1; -.
DR OMA; DFVPCPK; -.
DR OrthoDB; 21458at2759; -.
DR Proteomes; UP000019116; Chromosome 2D.
DR Proteomes; UP000280104; Chromosome ii.
DR Proteomes; UP000815260; Chromosome 2D.
DR GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-UniRule.
DR GO; GO:0000179; F:rRNA (adenine-N6,N6-)-dimethyltransferase activity; IBA:GO_Central.
DR GO; GO:0031167; P:rRNA methylation; IBA:GO_Central.
DR CDD; cd02440; AdoMet_MTases; 1.
DR Gene3D; 1.10.8.480; -; 1.
DR Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR InterPro; IPR001737; KsgA/Erm.
DR InterPro; IPR020596; rRNA_Ade_Mease_Trfase_CS.
DR InterPro; IPR020598; rRNA_Ade_methylase_Trfase_N.
DR InterPro; IPR011530; rRNA_adenine_dimethylase.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR NCBIfam; TIGR00755; ksgA; 1.
DR PANTHER; PTHR11727; DIMETHYLADENOSINE TRANSFERASE; 1.
DR PANTHER; PTHR11727:SF12; RIBOSOMAL RNA SMALL SUBUNIT METHYLTRANSFERASE, MITOCHONDRIAL; 1.
DR Pfam; PF00398; RrnaAD; 1.
DR SMART; SM00650; rADc; 1.
DR SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR PROSITE; PS01131; RRNA_A_DIMETH; 1.
DR PROSITE; PS51689; SAM_RNA_A_N6_MT; 1.
PE 3: Inferred from homology;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603, ECO:0000256|PROSITE-
KW ProRule:PRU01026}; Reference proteome {ECO:0000313|Proteomes:UP000019116};
KW RNA-binding {ECO:0000256|ARBA:ARBA00022884, ECO:0000256|PROSITE-
KW ProRule:PRU01026};
KW rRNA processing {ECO:0000256|ARBA:ARBA00022552,
KW ECO:0000256|RuleBase:RU362106};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691,
KW ECO:0000256|PROSITE-ProRule:PRU01026};
KW Transferase {ECO:0000256|ARBA:ARBA00022679, ECO:0000256|PROSITE-
KW ProRule:PRU01026}.
FT DOMAIN 46..216
FT /note="Ribosomal RNA adenine methylase transferase N-
FT terminal"
FT /evidence="ECO:0000259|SMART:SM00650"
FT REGION 282..302
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 39
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01026"
FT BINDING 41
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01026"
FT BINDING 66
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01026"
FT BINDING 88
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01026"
FT BINDING 116
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01026"
FT BINDING 131
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU01026"
SQ SEQUENCE 352 AA; 38515 MW; 5902FE2A117DD84A CRC64;
MNRAVSNSRA RLAAFSASSS LASEAWDGRF RLHKPRGQHL LTNPRVLDAI ARHAALRPGD
AVLEVGPGTG NLTARLLASP ADRVTAVEID PRMVDAVTAR FGALDLAHKL TVIQGDAMET
EFPKFDVCVA NIPYGISSPL IAKLLFGTYH FRTATLLLQK EFARRLVAMP GDSEYNRLAA
NVRMVADVKL LMDVSKRDFV PMPRVDSSLV EIQPRGAPPE VDLAEWLGFT RECFGQKNKT
LGAIFKQKRK ILDLLKRSQR SERCTSDAPG TGSGIILGVL DDDGNDEACS DEDGDSGDRA
AGFSTEEVGA FKERIAGALE STDLAGKRPS KMSNDELLYL LRLFNERGIW FQ
//
