ID W5K4X5_ASTMX Unreviewed; 2524 AA.
AC W5K4X5;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 2.
DT 27-MAR-2024, entry version 59.
DE RecName: Full=ubiquitinyl hydrolase 1 {ECO:0000256|ARBA:ARBA00012759};
DE EC=3.4.19.12 {ECO:0000256|ARBA:ARBA00012759};
OS Astyanax mexicanus (Blind cave fish) (Astyanax fasciatus mexicanus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Characiformes;
OC Characoidei; Characidae; Astyanax.
OX NCBI_TaxID=7994 {ECO:0000313|Ensembl:ENSAMXP00000002636.2, ECO:0000313|Proteomes:UP000018467};
RN [1] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RA Jeffery W., Warren W., Wilson R.K.;
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RX PubMed=25329095; DOI=10.1038/ncomms6307;
RA McGaugh S.E., Gross J.B., Aken B., Blin M., Borowsky R., Chalopin D.,
RA Hinaux H., Jeffery W.R., Keene A., Ma L., Minx P., Murphy D., O'Quin K.E.,
RA Retaux S., Rohner N., Searle S.M., Stahl B.A., Tabin C., Volff J.N.,
RA Yoshizawa M., Warren W.C.;
RT "The cavefish genome reveals candidate genes for eye loss.";
RL Nat. Commun. 5:5307-5307(2014).
RN [3] {ECO:0000313|Ensembl:ENSAMXP00000002636.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=Thiol-dependent hydrolysis of ester, thioester, amide, peptide
CC and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-
CC residue protein attached to proteins as an intracellular targeting
CC signal).; EC=3.4.19.12; Evidence={ECO:0000256|ARBA:ARBA00000707};
CC -!- SIMILARITY: Belongs to the peptidase C19 family.
CC {ECO:0000256|ARBA:ARBA00009085}.
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DR STRING; 7994.ENSAMXP00000002636; -.
DR Ensembl; ENSAMXT00000002636.2; ENSAMXP00000002636.2; ENSAMXG00000002577.2.
DR eggNOG; KOG1866; Eukaryota.
DR GeneTree; ENSGT00940000155375; -.
DR HOGENOM; CLU_000331_1_0_1; -.
DR InParanoid; W5K4X5; -.
DR Proteomes; UP000018467; Unassembled WGS sequence.
DR Bgee; ENSAMXG00000002577; Expressed in camera-type eye and 14 other cell types or tissues.
DR GO; GO:0004843; F:cysteine-type deubiquitinase activity; IEA:UniProtKB-EC.
DR CDD; cd02659; peptidase_C19C; 1.
DR Gene3D; 3.90.70.10; Cysteine proteinases; 1.
DR InterPro; IPR016024; ARM-type_fold.
DR InterPro; IPR021905; DUF3517.
DR InterPro; IPR038765; Papain-like_cys_pep_sf.
DR InterPro; IPR001394; Peptidase_C19_UCH.
DR InterPro; IPR018200; USP_CS.
DR InterPro; IPR028889; USP_dom.
DR PANTHER; PTHR24006; UBIQUITIN CARBOXYL-TERMINAL HYDROLASE; 1.
DR PANTHER; PTHR24006:SF827; UBIQUITINYL HYDROLASE 1; 1.
DR Pfam; PF12030; DUF3517; 1.
DR Pfam; PF00443; UCH; 1.
DR SUPFAM; SSF48371; ARM repeat; 1.
DR SUPFAM; SSF54001; Cysteine proteinases; 1.
DR PROSITE; PS00972; USP_1; 1.
DR PROSITE; PS00973; USP_2; 1.
DR PROSITE; PS50235; USP_3; 1.
PE 3: Inferred from homology;
KW Reference proteome {ECO:0000313|Proteomes:UP000018467}.
FT DOMAIN 1526..1927
FT /note="USP"
FT /evidence="ECO:0000259|PROSITE:PS50235"
FT REGION 1..63
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 941..972
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1561..1585
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 2446..2524
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..51
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 941..967
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2447..2461
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 2462..2505
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 2524 AA; 286795 MW; 78834B2480643872 CRC64;
MTATTRGSPV GGNDSQDQGQ APDGQSQPPL PQNQTSSPTS SNENSPVSPP EEQGQADCPP
QLEEEEPAFP HADLAKLDDM INRPRWVVPV LPKGELEVLL EAAIDLCKKG LDVKCEACQR
FFRDGLTISF TKILTDEAVS GWKFEIHRCI INNTHRLMEL CVTKLSQDWF PLLELLAMAT
NPHCKFHIYN GTRPSETVPA GVQLAEDELF ARPPDPRSPK GWLVDLINKF GTLNGFQILH
DRFMSGQVLN VQIIAALIKP FGQCYEFLTL HTVKKYFLPI IEMVPQFLEN LTDEELKKEA
KNEAKNDALS MIIKSLKNLA SRVPGQEETV KNMEIFRLKM ILRLLQISSF NGKMNALNEV
NKVISSVSYY THRHGNPEEA EWLTAERMAE WIQQNNILSI VLRDSLHQPQ YVEKLEKILR
FVIKEKALTL QDLDNIWAAQ AGKHEAIVKN VHDLLAKLAW DFSPEQLDHL FDCFKESWTN
ASKKQREKLL ELIRRLAEDD KDGVMAHKVL NLLWNLAHSD DVPVDIMDQA LSAHIKILDY
SCSQDRDTQK IQWIDRFIEE LRSNDKWVIP ALKQIREICS LFGEAPQNLS QTQRSPHVFY
RHDLINQLQH NHALVTLVAE NLSAYMENMR QFSKGWNSVF DGQLWLCAPQ AKQIWKCLAE
NAVFLCDREA CFKWYSKLMG DEPDLDPDIN KDFFENNVLQ LDPSLLTENG MKCFERFFKA
VNCREGKLVA KRRVYMMDDL ELIGLDYLWR VVIQGSDDIA SRAIDLLKEI YTNLGPKLQA
NQVEIHEDFI QSCFDRLKAS YDTLCVLDGD KDSINCARQE AIRMVRVLTV LREYITECDS
DYHEERTILP MSRAFRGKHI TLIVRFPNQG RQVDDLDIWS HTNDTIGSVR RCILNRIKAN
SAHTKIELFI GGDIVDPVDD RKLIGQLNLK DKTLITAKLT QVSANMPSSP DSSSDSSTGS
PGNHGNHYSD GPNPEVESCL PGVIMSLHLR YISFLWQVAD LGCNLNMPLL RDGARILMKL
MPPDNTTVEN LRAICLDHAK LGENSLSPTL DSRFFGPSPS QVLYLIEVVY ALLMPASGTL
GEDACDFQYN FLKSGGLPLV LSMLTRNNFL PNADMETRRG AYLNALKIAK LLLTAVGFGH
VKSVAEACQP VAEGTIPVSP INQATHDQAL VLQSALQNIP NPSAECMLRN VAIRLAQQIS
DEASKYIPDI CVIRAVQKIV WASGCGTIQL VFSSNEEISK IYEKTNAGNE PDAEDEQVCC
EALEVMTLCF ALIPTALDAL SKEKAWQTFI IDLLLHCQSK SVRQMAQEQF FLMATRCCMG
HRPLLFFITL LFTVLGSTAK ERAKHAGDYF TLLRHLLNYA YNSNINLPNA EVLLNNEIDW
LKRIKDEVKR TGETGVEETI LEGHIGVTKE LLAFQTPEKK YYIGCEKGGA NLIKELIDDF
IFPASNVYLQ YLKSGEFPTE QAIPVCSSPA TITAGFELLV ALAVGCVRNL KQIVDTLTDM
YYSGCEALTE WEYLPPVGPR PNKGFVGLKN AGATCYMNSV IQQLYMIPPI RNGILAIEGT
GSDVDDDMSG DEKQDNESNV DPRDEVFSYH HQFDDKPSLN KSEDRKEYNI GVLRHLQVIF
GHLASSRLQY YVPRGFWKQF RLWGEPVNLR EQHDALEFFN SLVDSLDEAL KALGHPAMLS
KVLGGSFADQ KICQGCPHRY ECEESFTTLN VDIRNHQNLL DSMEQYVKGD LLEGANAYHC
EKCNKKVDTV KRLLIKKLPP VLAIQLKRFD YDWERECAIK FNDYFEFPRE LDMEPYTVAG
VAKLEGSDMH PENQVIQQNE PSEPEPPCSS RYRLVGVLVH SGQASGGHYY SYIIQRNGSG
GEGERNRWYK FDDGDVTECK MDDDEEMKNQ CFGGEYMGEV FDHMMKRMSY RRQKRWWNAY
ILFYERMDSL DKDSELVKYI SELTLSTKPH QVKMPSAIEC SVRKQNVQFM HSRMQYSLEY
FQFIKKLLTC NSVYLNPPPG QDHLLPEAEE MAMISIQLAA RFLFSTGFHT KKVVRGPASD
WYDALCVLLR HSKNVRCWFA HNAIFAYANR FSEYLLECPS AEVRGAFSKL IVFIAHFSLQ
DGPYPSPVAS PGPSSQGCDN LSLSDHLFRA VLNLLRREVS EHGRHLQQYF NLFVMYANLG
LAEKTQLLKL SVPATFMLVA LDEGPGPPIK YQYAELGKLY TVVSQLVRCC DVASRMQSSI
NGNPPLPNPY GDPSLTQPIM PLQQLVADIL FVRTSYVKKI IEDCSNSEET IKLLRFCCWE
NPQFSSTVLS ELLWQVAYSY TYELRPYLDL LLQILLIEDS WQTHRIHNVL KGIPDDRDGL
FDTIQRSKNH YQKRAYQCIK CMVALFSNCS VAYQILQSNG DLKRKWTWAV EWLGDELERR
PYTGNAQYTY NNWSPPVQSN ETSNGYFLER SHSARMTLAK ACELCPEEEQ DDQDAPDDQE
SSPPEDTSLY PHSPGTQYQQ NNLPHGQPYT GPAAQHMNNP QRPGVRAQEN WEPTEEVPPA
QTKE
//
