ID W5KFS8_ASTMX Unreviewed; 855 AA.
AC W5KFS8;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 2.
DT 27-MAR-2024, entry version 54.
DE SubName: Full=HECT domain E3 ubiquitin protein ligase 3 {ECO:0000313|Ensembl:ENSAMXP00000006439.2};
OS Astyanax mexicanus (Blind cave fish) (Astyanax fasciatus mexicanus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Characiformes;
OC Characoidei; Characidae; Astyanax.
OX NCBI_TaxID=7994 {ECO:0000313|Ensembl:ENSAMXP00000006439.2, ECO:0000313|Proteomes:UP000018467};
RN [1] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RA Jeffery W., Warren W., Wilson R.K.;
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RX PubMed=25329095; DOI=10.1038/ncomms6307;
RA McGaugh S.E., Gross J.B., Aken B., Blin M., Borowsky R., Chalopin D.,
RA Hinaux H., Jeffery W.R., Keene A., Ma L., Minx P., Murphy D., O'Quin K.E.,
RA Retaux S., Rohner N., Searle S.M., Stahl B.A., Tabin C., Volff J.N.,
RA Yoshizawa M., Warren W.C.;
RT "The cavefish genome reveals candidate genes for eye loss.";
RL Nat. Commun. 5:5307-5307(2014).
RN [3] {ECO:0000313|Ensembl:ENSAMXP00000006439.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
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DR AlphaFoldDB; W5KFS8; -.
DR STRING; 7994.ENSAMXP00000006439; -.
DR Ensembl; ENSAMXT00000006439.2; ENSAMXP00000006439.2; ENSAMXG00000006259.2.
DR eggNOG; KOG0939; Eukaryota.
DR GeneTree; ENSGT00940000159923; -.
DR HOGENOM; CLU_002173_11_0_1; -.
DR InParanoid; W5KFS8; -.
DR OrthoDB; 2899647at2759; -.
DR Proteomes; UP000018467; Unassembled WGS sequence.
DR Bgee; ENSAMXG00000006259; Expressed in intestine and 14 other cell types or tissues.
DR GO; GO:0004842; F:ubiquitin-protein transferase activity; IEA:InterPro.
DR CDD; cd08666; APC10-HECTD3; 1.
DR Gene3D; 2.60.120.260; Galactose-binding domain-like; 1.
DR Gene3D; 3.30.2160.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.30.2410.10; Hect, E3 ligase catalytic domain; 1.
DR Gene3D; 3.90.1750.10; Hect, E3 ligase catalytic domains; 1.
DR InterPro; IPR004939; APC_su10/DOC_dom.
DR InterPro; IPR008979; Galactose-bd-like_sf.
DR InterPro; IPR000569; HECT_dom.
DR InterPro; IPR035983; Hect_E3_ubiquitin_ligase.
DR InterPro; IPR042469; HECTD3.
DR PANTHER; PTHR46654; E3 UBIQUITIN-PROTEIN LIGASE HECTD3; 1.
DR PANTHER; PTHR46654:SF1; E3 UBIQUITIN-PROTEIN LIGASE HECTD3; 1.
DR Pfam; PF03256; ANAPC10; 1.
DR Pfam; PF00632; HECT; 1.
DR SMART; SM01337; APC10; 1.
DR SMART; SM00119; HECTc; 1.
DR SUPFAM; SSF49785; Galactose-binding domain-like; 1.
DR SUPFAM; SSF56204; Hect, E3 ligase catalytic domain; 1.
DR PROSITE; PS51284; DOC; 1.
DR PROSITE; PS50237; HECT; 1.
PE 4: Predicted;
KW Reference proteome {ECO:0000313|Proteomes:UP000018467};
KW Ubl conjugation pathway {ECO:0000256|ARBA:ARBA00022786,
KW ECO:0000256|PROSITE-ProRule:PRU00104}.
FT DOMAIN 211..393
FT /note="DOC"
FT /evidence="ECO:0000259|PROSITE:PS51284"
FT DOMAIN 527..851
FT /note="HECT"
FT /evidence="ECO:0000259|PROSITE:PS50237"
FT REGION 49..86
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 49..67
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 817
FT /note="Glycyl thioester intermediate"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00104"
SQ SEQUENCE 855 AA; 96758 MW; F1AE7E5F027ADA16 CRC64;
MSPGQNPHTL LGRIRFLNRC TECFRTGEPL PESLCFVPRE VCYKICKDSS SNSGSSSASS
SAGNSGGKTL VPVWDHPNQP PTRKTARCTI EPKKGTCIRT SGEEYYNSHG LWVKISKEQL
EEYRSGVELE EGWILACKHA EGGDRLVPVE APDTASRQQQ LFGYDHKPCN RWEQVVDAES
CLHLGGQPKV AERDEAAVQK LRYVPSTWTF ECDEDLVHYF YDHVGKEDEN LGSVKQCVSS
VDVSSCSEDP SGGAGCLTDG DTETYWESDG MQGQHWIRLH MKRGTVVNKL ILTVDSTDDT
YMPKRVTVFG GEGDNLKKYS DVTIEDNLIG EVCVLEDMTS HLPIIEIRIE ECRDGGIDVR
IRGLKIKSSC ERDLGLNADV FQSPHLVRYP RLEGTPPDVL YRRALVILRF ISLLDGLLPH
LVPAWDYSLG TFNQIKSIKQ FLLLSKRRSA LIIQCLKDSE SSKPNFMPRL YINRRLAMEH
RDNPSLDPTC KNAVFTQVYE GLKPSDKYEK TLDYRWPARH DQWWECKFIA EGIIDQGGGF
RDSLADMSEE LCPSSAECAM PLPFFTRTSN QVAGEARDFY VPNPSCREFQ KYEWIGQLMG
AALRGKDFLV LALPGLVWKQ LTGESISWNE DFPAVDSVLV KLLEAMERMD KETFDFKFGQ
ELVYSTPLSD GRLVELIPGG GSVVVRYEDR MEFVRLVQKS RLEESKEQIA AIQAGLMKVV
PQAVLDLLTW QEVEKKVCGD PEITVEALKR LTRYEDLEQT DVRVQHLWEA LTNFTNEDRS
RFLRFVTGRS RLPAPIYVFP DKQGSETTDA LPQSSTCSST LYLPKYPSAK VCEEKLRYAA
YNCVAIDTDM SPWEE
//
