ID W5KNP2_ASTMX Unreviewed; 1636 AA.
AC W5KNP2;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 2.
DT 27-MAR-2024, entry version 61.
DE SubName: Full=Calcium channel, voltage-dependent, R type, alpha 1E subunit b {ECO:0000313|Ensembl:ENSAMXP00000009204.2};
GN Name=CACNA1E {ECO:0000313|Ensembl:ENSAMXP00000009204.2};
OS Astyanax mexicanus (Blind cave fish) (Astyanax fasciatus mexicanus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Characiformes;
OC Characoidei; Characidae; Astyanax.
OX NCBI_TaxID=7994 {ECO:0000313|Ensembl:ENSAMXP00000009204.2, ECO:0000313|Proteomes:UP000018467};
RN [1] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RA Jeffery W., Warren W., Wilson R.K.;
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RX PubMed=25329095; DOI=10.1038/ncomms6307;
RA McGaugh S.E., Gross J.B., Aken B., Blin M., Borowsky R., Chalopin D.,
RA Hinaux H., Jeffery W.R., Keene A., Ma L., Minx P., Murphy D., O'Quin K.E.,
RA Retaux S., Rohner N., Searle S.M., Stahl B.A., Tabin C., Volff J.N.,
RA Yoshizawa M., Warren W.C.;
RT "The cavefish genome reveals candidate genes for eye loss.";
RL Nat. Commun. 5:5307-5307(2014).
RN [3] {ECO:0000313|Ensembl:ENSAMXP00000009204.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000256|RuleBase:RU003808}; Multi-
CC pass membrane protein {ECO:0000256|RuleBase:RU003808}.
CC -!- SIMILARITY: Belongs to the calcium channel alpha-1 subunit (TC
CC 1.A.1.11) family. {ECO:0000256|RuleBase:RU003808}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR STRING; 7994.ENSAMXP00000009204; -.
DR Ensembl; ENSAMXT00000009204.2; ENSAMXP00000009204.2; ENSAMXG00000008956.2.
DR eggNOG; KOG2301; Eukaryota.
DR GeneTree; ENSGT00940000155601; -.
DR HOGENOM; CLU_000540_1_0_1; -.
DR InParanoid; W5KNP2; -.
DR Proteomes; UP000018467; Unassembled WGS sequence.
DR Bgee; ENSAMXG00000008956; Expressed in brain and 2 other cell types or tissues.
DR GO; GO:0005891; C:voltage-gated calcium channel complex; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0005245; F:voltage-gated calcium channel activity; IEA:InterPro.
DR Gene3D; 1.10.287.70; -; 4.
DR Gene3D; 6.10.250.2180; -; 1.
DR Gene3D; 6.10.250.2500; -; 1.
DR Gene3D; 1.20.120.350; Voltage-gated potassium channels. Chain C; 3.
DR InterPro; IPR031649; GPHH_dom.
DR InterPro; IPR005821; Ion_trans_dom.
DR InterPro; IPR014873; VDCC_a1su_IQ.
DR InterPro; IPR002077; VDCCAlpha1.
DR InterPro; IPR027359; Volt_channel_dom_sf.
DR PANTHER; PTHR45628; VOLTAGE-DEPENDENT CALCIUM CHANNEL TYPE A SUBUNIT ALPHA-1; 1.
DR PANTHER; PTHR45628:SF5; VOLTAGE-DEPENDENT R-TYPE CALCIUM CHANNEL SUBUNIT ALPHA-1E; 1.
DR Pfam; PF08763; Ca_chan_IQ; 1.
DR Pfam; PF16905; GPHH; 1.
DR Pfam; PF00520; Ion_trans; 4.
DR PRINTS; PR00167; CACHANNEL.
DR SMART; SM01062; Ca_chan_IQ; 1.
DR SUPFAM; SSF81324; Voltage-gated potassium channels; 4.
PE 3: Inferred from homology;
KW Calcium {ECO:0000256|ARBA:ARBA00022837, ECO:0000256|PIRSR:PIRSR602077-1};
KW Calcium channel {ECO:0000256|ARBA:ARBA00022673,
KW ECO:0000256|RuleBase:RU003808};
KW Calcium transport {ECO:0000256|ARBA:ARBA00022568,
KW ECO:0000256|RuleBase:RU003808};
KW Disulfide bond {ECO:0000256|ARBA:ARBA00023157};
KW Ion channel {ECO:0000256|ARBA:ARBA00023303};
KW Ion transport {ECO:0000256|ARBA:ARBA00023065};
KW Membrane {ECO:0000256|ARBA:ARBA00023136, ECO:0000256|SAM:Phobius};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|PIRSR:PIRSR602077-1};
KW Reference proteome {ECO:0000313|Proteomes:UP000018467};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW Transmembrane {ECO:0000256|ARBA:ARBA00022692, ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|ARBA:ARBA00022989,
KW ECO:0000256|SAM:Phobius}; Transport {ECO:0000256|ARBA:ARBA00022448};
KW Voltage-gated channel {ECO:0000256|ARBA:ARBA00022882,
KW ECO:0000256|RuleBase:RU003808}.
FT TRANSMEM 86..108
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 156..177
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 189..211
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 308..328
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 340..358
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 433..455
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 504..531
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 683..709
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 759..777
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 823..845
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 933..958
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1014..1032
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1053..1075
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1081..1104
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1125..1154
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT TRANSMEM 1231..1255
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 1389..1423
FT /note="Voltage-dependent calcium channel alpha-1 subunit
FT IQ"
FT /evidence="ECO:0000259|SMART:SM01062"
FT REGION 261..283
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1417..1543
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1427..1446
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1447..1461
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1462..1476
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1477..1510
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 170
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR602077-1"
FT BINDING 486
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR602077-1"
FT BINDING 905
FT /ligand="Ca(2+)"
FT /ligand_id="ChEBI:CHEBI:29108"
FT /evidence="ECO:0000256|PIRSR:PIRSR602077-1"
SQ SEQUENCE 1636 AA; 187311 MW; B1F20BA58A1FC381 CRC64;
MALYNPIPAR HNCLTVNRSL FIFAENNIIR KYARRIIILA TAGAHMNIPV DLRTLRAVRV
LRPLKLVSGI PLQIVLKSII KAMVPLLQIG LLLFFAILMF AIIGLEFYSG KLHHTCLTVD
SSEVEFACGV RRCPDKYDCN GTWIGPNDGI TQFDNILFAV LTVFQCITME GWTAVLYNTN
DALGPTWNWI YFIPLIIIGS FFVLNLVLGV LSEFAKERER VENRRAFMKL RRQQQVEREL
NGYRAWIDRA EVMLAEENKN SGRSALDGGG SRPPPSIRSA RRGPAAYLRR KERMLRFSIR
RMVKTDSFYW IVLSLVGLNT ICVAIVHHNQ PEWLTILQDY AEFVFLGLFL AEMFLKMYGL
GFRLYFHSSF NCFDCGQVIV GSIFEVVWGF FRPGMSFGIS VLRALRLLRI FKVTYWTSLR
NLVVSLMSSM KSIISLLFLL FLFIVVFALL GMQLFGGRFI FKDYTPANFD TFPAAIMTVF
QILTGEDWND VMYNGIRSQG GVQYGMWSSI YFIVLTLFGN TLLNVFLAIA VDNLANAQEL
TKEETPGKGG ACEEQPVNAF ANRRERRNKM KMSIWEQRAN QLRKRRQMAS REVLFPMDTP
VSGHHQPHAI TSMSPETSPA HLPESPMSVV MPLPEPPMAI PIPLPEPPES EPLPLLSSVA
EERTAPHRSN TERRHRVARI RRICHYVVTL RYFEMTILLV IVASSIALAA EDPVCTNSER
NRVLRYFDYV FTGVFTFEMI IKMIDQGLIL HDGSYFRDLW NILDFIVVVG ALIAFALNNK
GRDIKTIKSL RVLRVLRPLK TIKRLPKLKQ AVFDCVVTSL KNVFNILIVY KLFMFIFAVI
AVQLFKGKFF YCTDGSMGTQ RECGYYIDYG RDRKEVKKRE WKRHEFHYDN VMWALLTLFT
VSTGEGWPVL QHSVDVTEED HGPSRGNRME MSIFYVVYFV VFPFFFVNIF VALIIITFQE
QGDKMMEECS LEKNELFQRA CIDFAISAKP FTRYMPQNRQ TLQYRLWHFV VSPSFEYTVL
VMIALNTVVL MMKLQYHSAP AAYDTVLKHL NTAFTVLFSL ECILKIMAFG FMNYFRDTWN
IFDFITVLGS ITEIISVNTI NMSFLKLFRA ARLIKLLRQG YTIRILLWTF VQSFKALPYV
CLLIAMLFFI YAIIGMQVFG NIKLNDESHI NQHNNFKTFF SALMLLFSAT GESWQEIMLS
CLAGQECEPD PSIAPPFISP DHEGGCGTDF AYFYFVSFIF FSSFLMLNLF VAVIMDNFEY
LTRDSSILGP HHLDEFVRIW GEYDRAAGRI HYNAMYEMLT HMSPPLGLGK KCPAKIAYKR
LVLMNMPVDE DMSVHFTSTL MSLIRTALEI KIARGEDRVQ LDTELQKEIS VIWPHLSQKT
LDLLVPINKT DMTVGKIYAS MMIMDYFKQS KAKKLRQQAE EQSTVSHSEQ AHLERGRSKE
QRHLLSPKNS RFNSNERSSK SCSRSPSEER RHGLPRQGNS SDSPVPSTSE SSTPSGKRRL
SQTPTRTHPH ISYSPLVCHT HPSSQADDEE EEEEQSGPPM LGCEEFLGSS ARRYPSEPYL
ALQDEEHSPD SGPMETLTFE AAVATSLGRS NTITASVRTR SRLGWQVPNG HFRKRLAQSV
SVAGCEALSD TEDDKY
//
