ID W5KUJ4_ASTMX Unreviewed; 1250 AA.
AC W5KUJ4;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 2.
DT 27-MAR-2024, entry version 49.
DE RecName: Full=Alpha-mannosidase {ECO:0000256|RuleBase:RU361199};
DE EC=3.2.1.- {ECO:0000256|RuleBase:RU361199};
GN Name=MAN2B2 {ECO:0000313|Ensembl:ENSAMXP00000011256.2};
OS Astyanax mexicanus (Blind cave fish) (Astyanax fasciatus mexicanus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Characiformes;
OC Characoidei; Characidae; Astyanax.
OX NCBI_TaxID=7994 {ECO:0000313|Ensembl:ENSAMXP00000011256.2, ECO:0000313|Proteomes:UP000018467};
RN [1] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RA Jeffery W., Warren W., Wilson R.K.;
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RX PubMed=25329095; DOI=10.1038/ncomms6307;
RA McGaugh S.E., Gross J.B., Aken B., Blin M., Borowsky R., Chalopin D.,
RA Hinaux H., Jeffery W.R., Keene A., Ma L., Minx P., Murphy D., O'Quin K.E.,
RA Retaux S., Rohner N., Searle S.M., Stahl B.A., Tabin C., Volff J.N.,
RA Yoshizawa M., Warren W.C.;
RT "The cavefish genome reveals candidate genes for eye loss.";
RL Nat. Commun. 5:5307-5307(2014).
RN [3] {ECO:0000313|Ensembl:ENSAMXP00000011256.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- COFACTOR:
CC Name=Zn(2+); Xref=ChEBI:CHEBI:29105;
CC Evidence={ECO:0000256|RuleBase:RU361199};
CC Note=Binds 1 zinc ion per subunit. {ECO:0000256|RuleBase:RU361199};
CC -!- SIMILARITY: Belongs to the glycosyl hydrolase 38 family.
CC {ECO:0000256|ARBA:ARBA00009792, ECO:0000256|RuleBase:RU361199}.
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DR AlphaFoldDB; W5KUJ4; -.
DR Ensembl; ENSAMXT00000011256.2; ENSAMXP00000011256.2; ENSAMXG00000010962.2.
DR eggNOG; KOG1959; Eukaryota.
DR GeneTree; ENSGT01030000234638; -.
DR HOGENOM; CLU_004690_3_0_1; -.
DR Proteomes; UP000018467; Unassembled WGS sequence.
DR Bgee; ENSAMXG00000010962; Expressed in intestine and 14 other cell types or tissues.
DR GO; GO:0004559; F:alpha-mannosidase activity; IEA:UniProtKB-EC.
DR GO; GO:0030246; F:carbohydrate binding; IEA:InterPro.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0006013; P:mannose metabolic process; IEA:InterPro.
DR CDD; cd10811; GH38N_AMII_Epman_like; 1.
DR Gene3D; 2.60.40.1360; -; 1.
DR Gene3D; 3.20.110.10; Glycoside hydrolase 38, N terminal domain; 1.
DR Gene3D; 1.20.1270.50; Glycoside hydrolase family 38, central domain; 1.
DR Gene3D; 2.60.40.1180; Golgi alpha-mannosidase II; 1.
DR InterPro; IPR011013; Gal_mutarotase_sf_dom.
DR InterPro; IPR011330; Glyco_hydro/deAcase_b/a-brl.
DR InterPro; IPR011682; Glyco_hydro_38_C.
DR InterPro; IPR015341; Glyco_hydro_38_cen.
DR InterPro; IPR037094; Glyco_hydro_38_cen_sf.
DR InterPro; IPR000602; Glyco_hydro_38_N.
DR InterPro; IPR027291; Glyco_hydro_38_N_sf.
DR InterPro; IPR028995; Glyco_hydro_57/38_cen_sf.
DR InterPro; IPR013780; Glyco_hydro_b.
DR PANTHER; PTHR11607; ALPHA-MANNOSIDASE; 1.
DR PANTHER; PTHR11607:SF28; EPIDIDYMIS-SPECIFIC ALPHA-MANNOSIDASE; 1.
DR Pfam; PF09261; Alpha-mann_mid; 1.
DR Pfam; PF07748; Glyco_hydro_38C; 1.
DR Pfam; PF01074; Glyco_hydro_38N; 1.
DR SMART; SM00872; Alpha-mann_mid; 1.
DR SUPFAM; SSF88688; Families 57/38 glycoside transferase middle domain; 1.
DR SUPFAM; SSF74650; Galactose mutarotase-like; 1.
DR SUPFAM; SSF88713; Glycoside hydrolase/deacetylase; 1.
PE 3: Inferred from homology;
KW Coiled coil {ECO:0000256|SAM:Coils};
KW Glycosidase {ECO:0000256|ARBA:ARBA00023295, ECO:0000256|RuleBase:RU361199};
KW Hydrolase {ECO:0000256|RuleBase:RU361199};
KW Metal-binding {ECO:0000256|ARBA:ARBA00022723,
KW ECO:0000256|RuleBase:RU361199};
KW Reference proteome {ECO:0000313|Proteomes:UP000018467};
KW Zinc {ECO:0000256|ARBA:ARBA00022833, ECO:0000256|RuleBase:RU361199}.
FT DOMAIN 590..673
FT /note="Glycoside hydrolase family 38 central"
FT /evidence="ECO:0000259|SMART:SM00872"
FT COILED 114..166
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 1250 AA; 143231 MW; 1386300485BE47BC CRC64;
MSVMSRFDYS LLCNFPKCRA KLSGFAWVTA CSHVFCDQHG SAEFSRTPAI CPACSSTLSG
KLDIVRTELS PSEQYKAMVL VGLRPETVLD ISHRALAFWT YQVNQERRLM ECSLSRAEVQ
VVQMEKVIAQ QNQTRELEIN ALREEIASLN KRLVDYKQKY SEVSELLIKK NRQYQKLQGL
FEALRLRPRS AGSRDGLHHA SQAFATGLVR QHSPHGSPPF LSVGSEGDRL FSVEPESFKS
FFQFNSSPRD KISANGHQEP IQAFVIPHSH MDVGWVYTVQ ESMHAYASNV YTTVVEELSR
VKGRRFIAVE QEFFRLWWDT AASQWHKKQV RQLLQEGRLE FIIGGQVMHD ESVTDIDDAI
LQLTEGHGFL YETFGVRPHF SWHVDPFGAS ATTPVLFALA GFDAHLISRI DYDLKDVMQK
KKKLQFVWRG SPSLADTQEI FTHTMDQYSY CTPSYIPFSN RSGFYWNGVA MFPDPPKDGV
YPNMSLPVTK ETVEPYAQTM VDNIKQRAQW FQTNHVLWPW GCDKQFYNAS VQFTNMDMLM
NYINKHSTEF GVTVQYATLK EYFQAVHQSN LSWEVRGSQD FLPYSTEPFQ AWTGFYASRN
VLKGVARRAS SLLHSAESLF VRYRIKYPEG PVQKEWALDK LKALRWAVSE VQHHDAITGT
ESPKVADMYM EHLNQSMMGV QELLAAMFLL PQDPDTASAK YRSNTKDEQP SKDLEQHIIV
YNPLAWNVTT YINVSVKYSM ALVYDDNGKV VPAQIQPSAE SPADYDLFIM VELGGLQYRK
YLIQFPQSEC KKGSSCGITH AARVVKFERR DVSEWKKRGR KLLPVLNDCY KLLFDQETNL
LHSITNRLEG QKVIIHQDFW EYDANGDVHA GPISDNYIFT ANGSAVPAYK SVEMEIIPGK
IISEVRQYFY REASDKNYTY AMVTRVPVGF KDKLLCQRLD QSYALGPLVV NREAVLRTTT
SLSNKKTLYT DNNGYQMLKR EHKQYINNTV SRNYYPMVRQ AFIEDEDSCR LSLLSERAHG
VASLAEGEIE VMLHRRLWNN QEWNRGYNLT LNDSSVVRPS IWMMLGSSAA VAALSQQGAL
YLQHRPVVLP IDKPQKPWSE HERWNGPSVQ PVVLPPNLHM QYLGIPGWDY GPNHTQHLQN
INSGVKTNPE ADFDRVLLRI AHLYEKGEDP VLSKPATINL KEILQGLGEV KAVQERSLTG
TWDISDLQRW SWRTTEKTGN KKTSSSSRRV TMDFNVTISP KEIRTFFIHF
//
