ID W5L7L2_ASTMX Unreviewed; 3895 AA.
AC W5L7L2;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 05-DEC-2018, sequence version 2.
DT 27-MAR-2024, entry version 56.
DE SubName: Full=Dynein, axonemal, heavy chain 12 {ECO:0000313|Ensembl:ENSAMXP00000015824.2};
GN Name=DNAH12 {ECO:0000313|Ensembl:ENSAMXP00000015824.2};
OS Astyanax mexicanus (Blind cave fish) (Astyanax fasciatus mexicanus).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Characiformes;
OC Characoidei; Characidae; Astyanax.
OX NCBI_TaxID=7994 {ECO:0000313|Ensembl:ENSAMXP00000015824.2, ECO:0000313|Proteomes:UP000018467};
RN [1] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE.
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RA Jeffery W., Warren W., Wilson R.K.;
RL Submitted (MAR-2013) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Proteomes:UP000018467}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=female {ECO:0000313|Proteomes:UP000018467};
RX PubMed=25329095; DOI=10.1038/ncomms6307;
RA McGaugh S.E., Gross J.B., Aken B., Blin M., Borowsky R., Chalopin D.,
RA Hinaux H., Jeffery W.R., Keene A., Ma L., Minx P., Murphy D., O'Quin K.E.,
RA Retaux S., Rohner N., Searle S.M., Stahl B.A., Tabin C., Volff J.N.,
RA Yoshizawa M., Warren W.C.;
RT "The cavefish genome reveals candidate genes for eye loss.";
RL Nat. Commun. 5:5307-5307(2014).
RN [3] {ECO:0000313|Ensembl:ENSAMXP00000015824.2}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- SIMILARITY: Belongs to the dynein heavy chain family.
CC {ECO:0000256|ARBA:ARBA00008887}.
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DR STRING; 7994.ENSAMXP00000015824; -.
DR Ensembl; ENSAMXT00000015824.2; ENSAMXP00000015824.2; ENSAMXG00000014371.2.
DR eggNOG; KOG3595; Eukaryota.
DR GeneTree; ENSGT00940000154280; -.
DR HOGENOM; CLU_000038_0_1_1; -.
DR InParanoid; W5L7L2; -.
DR Proteomes; UP000018467; Unassembled WGS sequence.
DR Bgee; ENSAMXG00000014371; Expressed in testis and 5 other cell types or tissues.
DR GO; GO:0005930; C:axoneme; IEA:UniProt.
DR GO; GO:0030286; C:dynein complex; IEA:UniProtKB-KW.
DR GO; GO:0005874; C:microtubule; IEA:UniProtKB-KW.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0016887; F:ATP hydrolysis activity; IEA:InterPro.
DR GO; GO:0045505; F:dynein intermediate chain binding; IEA:InterPro.
DR GO; GO:0051959; F:dynein light intermediate chain binding; IEA:InterPro.
DR GO; GO:0008569; F:minus-end-directed microtubule motor activity; IEA:InterPro.
DR GO; GO:0007018; P:microtubule-based movement; IEA:InterPro.
DR CDD; cd00009; AAA; 1.
DR Gene3D; 1.10.287.2620; -; 1.
DR Gene3D; 1.10.472.130; -; 1.
DR Gene3D; 1.10.8.1220; -; 1.
DR Gene3D; 1.10.8.710; -; 1.
DR Gene3D; 1.20.1270.280; -; 1.
DR Gene3D; 1.20.58.1120; -; 1.
DR Gene3D; 1.20.920.20; -; 1.
DR Gene3D; 1.20.920.30; -; 1.
DR Gene3D; 3.10.490.20; -; 1.
DR Gene3D; 6.10.140.1060; -; 1.
DR Gene3D; 1.20.140.100; Dynein heavy chain, N-terminal domain 2; 1.
DR Gene3D; 3.20.180.20; Dynein heavy chain, N-terminal domain 2; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 5.
DR Gene3D; 1.10.8.720; Region D6 of dynein motor; 1.
DR InterPro; IPR003593; AAA+_ATPase.
DR InterPro; IPR035699; AAA_6.
DR InterPro; IPR035706; AAA_9.
DR InterPro; IPR041658; AAA_lid_11.
DR InterPro; IPR042219; AAA_lid_11_sf.
DR InterPro; IPR026983; DHC_fam.
DR InterPro; IPR041589; DNAH3_AAA_lid_1.
DR InterPro; IPR042222; Dynein_2_N.
DR InterPro; IPR043157; Dynein_AAA1S.
DR InterPro; IPR041466; Dynein_AAA5_ext.
DR InterPro; IPR041228; Dynein_C.
DR InterPro; IPR043160; Dynein_C_barrel.
DR InterPro; IPR024743; Dynein_HC_stalk.
DR InterPro; IPR024317; Dynein_heavy_chain_D4_dom.
DR InterPro; IPR004273; Dynein_heavy_D6_P-loop.
DR InterPro; IPR013602; Dynein_heavy_linker.
DR InterPro; IPR042228; Dynein_linker_3.
DR InterPro; IPR027417; P-loop_NTPase.
DR PANTHER; PTHR45703; DYNEIN HEAVY CHAIN; 1.
DR PANTHER; PTHR45703:SF1; DYNEIN HEAVY CHAIN; 1.
DR Pfam; PF12774; AAA_6; 1.
DR Pfam; PF12775; AAA_7; 1.
DR Pfam; PF12780; AAA_8; 1.
DR Pfam; PF12781; AAA_9; 1.
DR Pfam; PF17857; AAA_lid_1; 1.
DR Pfam; PF18198; AAA_lid_11; 1.
DR Pfam; PF08393; DHC_N2; 1.
DR Pfam; PF17852; Dynein_AAA_lid; 1.
DR Pfam; PF18199; Dynein_C; 1.
DR Pfam; PF03028; Dynein_heavy; 1.
DR Pfam; PF12777; MT; 1.
DR SMART; SM00382; AAA; 2.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 4.
PE 3: Inferred from homology;
KW ATP-binding {ECO:0000256|ARBA:ARBA00022840};
KW Cell projection {ECO:0000256|ARBA:ARBA00023273};
KW Cilium {ECO:0000256|ARBA:ARBA00023069};
KW Coiled coil {ECO:0000256|ARBA:ARBA00023054, ECO:0000256|SAM:Coils};
KW Cytoplasm {ECO:0000256|ARBA:ARBA00022490};
KW Cytoskeleton {ECO:0000256|ARBA:ARBA00023212};
KW Dynein {ECO:0000256|ARBA:ARBA00023017};
KW Microtubule {ECO:0000256|ARBA:ARBA00022701};
KW Motor protein {ECO:0000256|ARBA:ARBA00023175};
KW Nucleotide-binding {ECO:0000256|ARBA:ARBA00022741};
KW Reference proteome {ECO:0000313|Proteomes:UP000018467}.
FT DOMAIN 1213..1352
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT DOMAIN 1850..1998
FT /note="AAA+ ATPase"
FT /evidence="ECO:0000259|SMART:SM00382"
FT REGION 685..710
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COILED 537..568
FT /evidence="ECO:0000256|SAM:Coils"
FT COILED 2702..2743
FT /evidence="ECO:0000256|SAM:Coils"
SQ SEQUENCE 3895 AA; 446049 MW; CB229B9A9332FC61 CRC64;
MEDALKKYRA LPPIPSLPVQ ASDSSLPEHI RTVRRKLLNH RRSKLQQKEM NYMFLKECVE
RRPIVPIQQS WLDDMLTRVS PQLRQGRQEL LENLCDEVRE SFHSCILKHT GKHTSVDFIS
PRVFSLVRTL DFARPWHDSF IRSRKLIKKN LHILHPVMKI ILDICYVTFS DLLLVDLSAC
RSSGPVSCGS LKNKVALECE RIETKLMNTW FPKVIHLLTS KDTVQGITTE KLDAFYNCAS
TLLSNQLKAV VERSVVAFVN LFDPLSTHKL PLFRMNLTFE EEKMDFYPSF QDMEEAVLEI
FTSISNTMQK VQTVQSWLSE TNNSVIDAKL VDHVRAWAET TLKNAVWKNL EAPNQHFQNY
SNNYDWLVNR TAQDLVEKFI QEEHSFDQYT QVIEKFQALS REISSLPSVA HFDMIRLDCE
ELKQGLAKKA ETFAQILLEQ LINTQRQQCL EICEEFETIQ KKAMKKPETT EDMIEMIAYI
SHARTKGLEE LHSKIMETHQ RFTYLLHVHT FSAEDLELNS KMVLWPQNIN PIFDLNNELL
EQAKQKGEQE LQGKREKLML KLEKLGRRME EFAECSELDM MQQYVTEIKT VQKWLQEVED
SISFINKEEA LYKWDQTVYP EIDVMKENIE PYQKVFGLVL KWQRTEKRWM DGSFLDLNGE
SMEAELDEFF REIYKMLKSF QQKQKKAEQG RERQVAMKRR PGEGDDDHLE SPTALMCIKV
IEQIKEFKEH IPMVSILCNP GIRARHWEQM SEIVNHDLTP NSGTTLRKVL KQNLSAYMEH
FETISAAASK EFSLEKALQN MVETWDIISF SHHAHRESGV SILTAVDEIQ TMLDDQIVKT
QTMRGSPFIK PFEKEIKAWE ERLIRIQDTI DEWLKVQAQW LYLEPIFSSQ DIMQQMPEEG
QLFQTVDKHW REVMRHCVKD PKVLPATSLT GLLEKLQESN NLLEQIMKGL NAYLEKKRLF
FPRFFFLSND EMLEILSETK DPLRVQPHLK KCFEGIAKLD FLPNLDIQAM YSSEGERVEL
IQLISTSEAR GAVEKWLVQV EDVMLSSIRD VINRSRLAYP ETKRSQWVRE WPGQVVLCTS
QMYWTQEVHE AIRSGVGGLK NYYNQLQSQL KDIVELVRGK LPKQTRTTLG ALVTIDVHAR
DVVMEMIEKG VSHETDFQWL AQLRYYWNND NVRVCIINCD VKYAYEYLGN SPRLVITPLT
DRCYRTLIGA FYLNLGGAPE GPAGTGKTET TKDLAKALAV QCVVFNCSDG LDYLAMGKFF
KGLASSGAWA CFDEFNRIEL EVLSVVAQQI LCIQRAIEMK LEFFDFEGTE LRLNPNCFVA
ITMNPGYAGR SELPDNLKVL FRTVAMMVPN YALIAEISLY SYGFLNAKPL SVKIVMTYRL
CSEQLSSQFH YDYGMRAVKA VLVAAGNLKL KFPDENEDIL LLRSIKDVNE PKFLSHDIPL
FNGITSDLFP GISLPAADYQ LFLDCAQECC KKHNVQPVKV FLDKMIQTYE MMIVRHGFML
VGEPFAGKTK VLHVLADTLT LMNERGYNDE EKVQYRTVNP KSITMGQLFG QFDPVSHEWT
DGIVANTFRE YASAETPDRK WVVFDGPIDT LWIESMNTVL DDNKKLCLMS GEIIQMSNQM
SLIFEAMDLS QASPATVSRC GMIYMEPSQL GWLPLVTSWL NTLPQPLQTQ ENSTLLLELF
NWLIPPALRM LRKNCREVVS TTNSNTVVSL TRLFEMLLTE PVTEEPGNKN IRTWIMAAFA
FSLVWSIGGS CDRDSRERFD QFLREILSGK LEEHPIPAAV SKWECPFNDK GLVYDYSYEF
KGKGCWIHWN ESIKNVTLGD KNTKVQDIIV PTIDTIFCTL FTTLIIFFSP FRPLLFVGPT
GTGKSVYVKE KLMNNLNKER YLPFFINFSA RTSANQSQNI IMARLDKRRK GVFGPPMGKK
CIIFVDDMNM PAMEVFGAQP PIELLRQYFD HGNWYDLKDT SKITLVDVQL ISAMGPPGGG
RNAVTPRFLR HFNICSMNAF SDDTMSRIFS NVVAFYLRNN DFPNDCFSIG NQIVAATLEV
YKKAMENLLP TPAKSHYTFN LRDFSRVVQG CLLLKKESLE NKRTMIRLFV HEVFRVFYDR
LVDDQDRDWL YKLMKNIVKE HFKESFDTVF EHLKLCEEDM RSLLFGDYMT PDVEASERLY
AEVPSMESFS QVVESYLTEY NQTHKNRMNL VIFRYVLEHL SRISRVLKQP GGNALLVGVG
GSGRQSITRL ATFMADMTLF QPEISKNYGM NEWREDLKLL LKKAGVKGQK TVFLLTDAQI
KEEAFLEDVD SVLNTGEVPN LFAVDEKQEI MEVMHAVRPI AQAGNKSLEF SPLALFAFFV
NRCRENLHVV VAFSPIGDAF RNRLRQFPSL INCCTINWFQ PWPEEALERV ANKFLETLEM
SDHERQEVML ICKTFHTSAI HLSEKFLSEL GRHNYVTPTS YLELIAAFRL LLTNKRDTVM
NAKKRYTNGL DKLAFAESQV GEMKKELVDL QPKLEQAKID NIKMVQVIEV ESVQVEAKSK
VVRVEEEAAS QKAAEAQALK NECESDLAEA IPALEAALSA LDTLKPSDVT IVKSMKNPPS
GVKLVMAAVC VMKDIKPEKI NDPAGTGQKI LDYWGPSKKL LGDMNFLRDL KVYDKDNIPA
PVMHKIRSEY MTNPDFDPTK VAKASSAAEG LCKWITAMEV YDRVAKVVAP KKANLIVAQQ
SLAATMALLN QKRAELKEVE DRLAALKKTL EEKTEEQAQL QFQVDLCARK LERAEKLIGG
LGGEKTRWSK AADDLQNTYD NLTGDVLISA GVIAYLGAFT AGFRQNCTKE WTKLCKSKNI
PSSDDFSLSK TLGDPIKIRA WNISGLPTDS FSIDNGVIVS NSRRWPLMID PQGQANKWVK
NSERENNLNV IKLTDADYMR TLENCIQFGT PLLLENVGEE LDPSLEPLLL KQIFKQGGMD
CIRLGESVIE FSSDFRFYIT TKLRNPHYLP ELATKVSLLN FMITPEGLED QLLGIVVAKE
RPELEEERNA LILQSAANKK QLKEIEDKIL ETLQSSEGNI LEDESAIQIL DSAKIMSNEI
SKKQQIAEKT ELKIAESREG YRPIAKHSSI LFFSIADLAN IDPMYQYSLN WFVNLYINSI
QDSNKSKILE KRLRYLINHF TYNLYCNVCR SLFEKDKLLF SFLLCSNLLL AKKEIEYAEF
MFLLTGGVGL QNNVPNPDPN WLQDKSWDEI CRASDLPGLQ GLRYPPFCHF LPIYDSKEPY
NTPLPKPWCD KLSDFQKMII YRCLRPDKIE PAISNYITGK LGKKFVEPPP FDLSKSYNDS
NSTIPLVFVL SPGADPMASL LKFANDKNMG GANFKSISLG QGQGPIAVKM IQSAMKEGTW
VCLQNCHLAV SWMSTLEKIC EDFSPETCHP DFRLWLTSYP SPMFPVTILQ NGVKMTNEPP
TGLRLNLLQS YLSDPVSDPQ FFSACSNKEL VWEKLLYGVC FFHALVQERK KFGPLGWNIP
YGFNESDLRI SIRQLQLFVN SYEEVPFEAI TYLTGECNYG GRVTDDWDRR LLLTILADFY
NKDIIENPRY MFSPSGKYYS PPKSIYEDYV EFIRNLPATQ HPEVFGMHEN VDISKDLQQT
KLLFDSLILT QGGGSKGGAS SGGDSTLMDI ANDIRTKLPE NFNIDSALNK FPVLYEESMN
TVLVQEMERY NNLCNTIRVS LQNLLKAIKG LVVMDAELEA IAGSLLVGKV PEKWAKRSYP
SLKPLGSYIT DFLTRLKFLQ DWYDTTKPNV FWLSGFFFTQ AFLTGAMQNY ARKYSIPIDL
LSFDYEVLPF DTSATSPEDG VYVHGLFLDG ARWDKKSGVL AEQHPKVLFD SVPIIWIKPK
HCCTFLLLSC TSERKGTLST TGHSTNFVIS IMLPTSKRPQ HWIKRGVAML CQLDN
//
