ID W5MQC5_LEPOC Unreviewed; 1389 AA.
AC W5MQC5;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 57.
DE SubName: Full=Neuropathy target esterase-like {ECO:0000313|Ensembl:ENSLOCP00000010584.1};
GN Name=PNPLA6 {ECO:0000313|Ensembl:ENSLOCP00000010584.1};
OS Lepisosteus oculatus (Spotted gar).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Holostei; Semionotiformes; Lepisosteidae;
OC Lepisosteus.
OX NCBI_TaxID=7918 {ECO:0000313|Ensembl:ENSLOCP00000010584.1, ECO:0000313|Proteomes:UP000018468};
RN [1] {ECO:0000313|Proteomes:UP000018468}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Di Palma F., Alfoldi J., Johnson J., Berlin A., Gnerre S., Jaffe D.,
RA MacCallum I., Young S., Walker B.J., Lander E.S., Lindblad-Toh K.;
RT "The Draft Genome of Lepisosteus oculatus.";
RL Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSLOCP00000010584.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-(9Z-octadecenoyl)-sn-glycero-3-phosphocholine + H2O = (9Z)-
CC octadecenoate + H(+) + sn-glycerol 3-phosphocholine;
CC Xref=Rhea:RHEA:40807, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16870, ChEBI:CHEBI:28610, ChEBI:CHEBI:30823;
CC Evidence={ECO:0000256|ARBA:ARBA00024569};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40808;
CC Evidence={ECO:0000256|ARBA:ARBA00024569};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-hexadecanoyl-sn-glycero-3-phosphate + H2O = H(+) +
CC hexadecanoate + sn-glycerol 3-phosphate; Xref=Rhea:RHEA:49092,
CC ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:57518, ChEBI:CHEBI:57597;
CC Evidence={ECO:0000256|ARBA:ARBA00000355};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:49093;
CC Evidence={ECO:0000256|ARBA:ARBA00000355};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=1-hexadecanoyl-sn-glycero-3-phosphocholine + H2O = H(+) +
CC hexadecanoate + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:40435,
CC ChEBI:CHEBI:7896, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378,
CC ChEBI:CHEBI:16870, ChEBI:CHEBI:72998;
CC Evidence={ECO:0000256|ARBA:ARBA00000597};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:40436;
CC Evidence={ECO:0000256|ARBA:ARBA00000597};
CC -!- CATALYTIC ACTIVITY:
CC Reaction=a 1-acyl-sn-glycero-3-phosphocholine + H2O = a fatty acid +
CC H(+) + sn-glycerol 3-phosphocholine; Xref=Rhea:RHEA:15177,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16870,
CC ChEBI:CHEBI:28868, ChEBI:CHEBI:58168; EC=3.1.1.5;
CC Evidence={ECO:0000256|ARBA:ARBA00000150};
CC PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:15178;
CC Evidence={ECO:0000256|ARBA:ARBA00000150};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane
CC {ECO:0000256|ARBA:ARBA00004643}; Single-pass type III membrane protein
CC {ECO:0000256|ARBA:ARBA00004643}. Membrane
CC {ECO:0000256|ARBA:ARBA00004183}; Single-pass type III membrane protein
CC {ECO:0000256|ARBA:ARBA00004183}.
CC -!- SIMILARITY: Belongs to the NTE family. {ECO:0000256|ARBA:ARBA00006636}.
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DR EMBL; AHAT01007547; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AHAT01007548; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_015204592.1; XM_015349106.1.
DR RefSeq; XP_015204593.1; XM_015349107.1.
DR STRING; 7918.ENSLOCP00000010584; -.
DR Ensembl; ENSLOCT00000010599.1; ENSLOCP00000010584.1; ENSLOCG00000008681.1.
DR GeneID; 102698342; -.
DR KEGG; loc:102698342; -.
DR CTD; 10908; -.
DR eggNOG; KOG2968; Eukaryota.
DR GeneTree; ENSGT00940000159130; -.
DR HOGENOM; CLU_000960_1_0_1; -.
DR InParanoid; W5MQC5; -.
DR OMA; CIEDLWI; -.
DR OrthoDB; 5303733at2759; -.
DR Proteomes; UP000018468; Linkage group LG6.
DR Bgee; ENSLOCG00000008681; Expressed in testis and 13 other cell types or tissues.
DR GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0004622; F:lysophospholipase activity; IBA:GO_Central.
DR GO; GO:0102545; F:phosphatidyl phospholipase B activity; IEA:UniProtKB-EC.
DR GO; GO:0009948; P:anterior/posterior axis specification; IEA:Ensembl.
DR GO; GO:0007409; P:axonogenesis; IEA:Ensembl.
DR GO; GO:0016042; P:lipid catabolic process; IEA:UniProtKB-UniRule.
DR GO; GO:0030514; P:negative regulation of BMP signaling pathway; IEA:Ensembl.
DR GO; GO:0046470; P:phosphatidylcholine metabolic process; IEA:InterPro.
DR CDD; cd00038; CAP_ED; 3.
DR CDD; cd07225; Pat_PNPLA6_PNPLA7; 1.
DR Gene3D; 3.40.1090.10; Cytosolic phospholipase A2 catalytic domain; 2.
DR Gene3D; 2.60.120.10; Jelly Rolls; 3.
DR InterPro; IPR016035; Acyl_Trfase/lysoPLipase.
DR InterPro; IPR000595; cNMP-bd_dom.
DR InterPro; IPR018490; cNMP-bd_dom_sf.
DR InterPro; IPR001423; LysoPLipase_patatin_CS.
DR InterPro; IPR002641; PNPLA_dom.
DR InterPro; IPR014710; RmlC-like_jellyroll.
DR PANTHER; PTHR14226; NEUROPATHY TARGET ESTERASE/SWISS CHEESE D.MELANOGASTER; 1.
DR PANTHER; PTHR14226:SF26; PATATIN-LIKE PHOSPHOLIPASE DOMAIN-CONTAINING PROTEIN 6; 1.
DR Pfam; PF00027; cNMP_binding; 3.
DR Pfam; PF01734; Patatin; 1.
DR SMART; SM00100; cNMP; 3.
DR SUPFAM; SSF51206; cAMP-binding domain-like; 3.
DR SUPFAM; SSF52151; FabD/lysophospholipase-like; 2.
DR PROSITE; PS50042; CNMP_BINDING_3; 3.
DR PROSITE; PS51635; PNPLA; 1.
DR PROSITE; PS01237; UPF0028; 1.
PE 3: Inferred from homology;
KW Hydrolase {ECO:0000256|ARBA:ARBA00022801};
KW Lipid degradation {ECO:0000256|ARBA:ARBA00022963};
KW Lipid metabolism {ECO:0000256|ARBA:ARBA00023098};
KW Membrane {ECO:0000256|SAM:Phobius};
KW Reference proteome {ECO:0000313|Proteomes:UP000018468};
KW Transmembrane {ECO:0000256|SAM:Phobius};
KW Transmembrane helix {ECO:0000256|SAM:Phobius}.
FT TRANSMEM 32..55
FT /note="Helical"
FT /evidence="ECO:0000256|SAM:Phobius"
FT DOMAIN 167..294
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
FT DOMAIN 499..581
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
FT DOMAIN 609..714
FT /note="Cyclic nucleotide-binding"
FT /evidence="ECO:0000259|PROSITE:PS50042"
FT DOMAIN 948..1114
FT /note="PNPLA"
FT /evidence="ECO:0000259|PROSITE:PS51635"
FT REGION 411..440
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 1326..1389
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 411..426
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1326..1351
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1366..1381
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 1389 AA; 155395 MW; EB3854E033E27BDC CRC64;
MGQSNSEPDT DSSDDGLMNI KTFVEDQLQT SMMVGIVIGA GISIILIAIL IFFVLRRVQL
RKLQAQEAPK YRFRKRDKVM FYGRKIMRKV SQSTSSLVGT TASRPRLKKK QKMLNIAKKI
LRFKKEMPTL QMKEPPPSVL EADLTEFDVA NSHLPSEVLY MLKNVRVLGH FEKPLFLELC
KHMVFLQFQQ GEYVFRPGQP DSSIYVVQDG KLELCLTGQD GKDGVVKEVY PGDSVHSLLS
ILDVITGHQK PYRTVSARAA EMSTVLRLPV EAFLSIFEKY PESLVRVVQI IMVRLQRVTV
LALHNYLGLT NELFSHEMQP LRLLPQASHP SRTSPVRHSK RFISTSICED HRDSTAKSGE
LAGGDHVRDG ATVPTLSRAI SMPVDIAGIQ KGLRSDFDMA YERGRISVSL QEDGTASATP
TFSRSVSQEP RERKSVTVEE QPSGVYLYPE EDVLDSLYGP LHGKHANNLF EEAKNEVLKL
MRIEDAALLN GRITLHHAKA GSVLARQGDQ DVSLHFVLSG CLHVYQRMID KLEDVCLFIT
QPGEMVGQLA VLTGEPLIFT IKAVRDCTFL KISKSDFYEI MREQPSVVLS AAHTVAVRMS
AFVRQMDFAI DWMAVEAGRA LYRQGDQSDC TYIVLNGRLR SVIRKANGKK ELVGEYGRGD
LIGVVEALTR QPRATTVHAV RDTELVKLPE GTLNNIKRRY PQVVTRLIHL LGQKILGNLQ
QVRGPLSGSA LGLSAVGTSH DVTNPASNLS TVAVLPICDD VPVNAFNLEL SHALNAIGPT
LLLTSDIIRE RLGASALDSI HEYRLSGWLA QQEDINRIVL YQTDSTMTPW TQRCIRQADC
ILIIGLGDQE PTLGELEQML ENTAVRALKQ LVLLHKEDGP GPARTVEWLN MRSWCSGHLH
LKCPRRVFSR RSPTRLREMY EKVFEKTVDR YSDFSRLARV LTGNSIALVL GGGGARGCSH
VGVIKAMEEA GIPIDIVGGT SIGSFIGALY AEERSAVRTK QRAREWSKSM NSVFKTVLDL
TYPITSMFSG SAFNTSISKV FEDKQIEDLW LPYFNVTTDI TASAMRVHKD GCVWRYVRAS
ASYTPYLPPL CDPKDGHLLV DGCYVNNVPG SLWRYVRASM TLSGYLPPLC DPKDGNLLMD
GGYINNLPAD MARNMGAKTV IAIDVGSQDE TDLCNYGDCL SGWWLLWKRI NPWAEKVKVP
DMAEIQSRLA YVSCVRQLEV VKNSAYCEYI RPPIDRFKTM DFGKFDEIYD VGYQHGKVVF
NAWSRGDIID NMLKDRRSAD FNESKKADIC PGADFTDLAE IVSRIEPVHS YVSAEAEESD
CLTEYEDDGM DTVREEEGEE EEEEPEEQDD QSPGEWGQNG VFEVDELKSV RNRRKKTSDS
NTCSEISDS
//
