ID W5N4W0_LEPOC Unreviewed; 341 AA.
AC W5N4W0;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 44.
DE RecName: Full=tRNA methyltransferase 10 homolog B {ECO:0000256|ARBA:ARBA00035688};
DE EC=2.1.1.221 {ECO:0000256|ARBA:ARBA00012797};
DE AltName: Full=RNA (guanine-9-)-methyltransferase domain-containing protein 3 {ECO:0000256|ARBA:ARBA00035712};
DE AltName: Full=tRNA (guanine(9)-N(1))-methyltransferase TRMT10B {ECO:0000256|ARBA:ARBA00035725};
OS Lepisosteus oculatus (Spotted gar).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC Actinopterygii; Neopterygii; Holostei; Semionotiformes; Lepisosteidae;
OC Lepisosteus.
OX NCBI_TaxID=7918 {ECO:0000313|Ensembl:ENSLOCP00000015669.1, ECO:0000313|Proteomes:UP000018468};
RN [1] {ECO:0000313|Proteomes:UP000018468}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Di Palma F., Alfoldi J., Johnson J., Berlin A., Gnerre S., Jaffe D.,
RA MacCallum I., Young S., Walker B.J., Lander E.S., Lindblad-Toh K.;
RT "The Draft Genome of Lepisosteus oculatus.";
RL Submitted (DEC-2011) to the EMBL/GenBank/DDBJ databases.
RN [2] {ECO:0000313|Ensembl:ENSLOCP00000015669.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=guanosine(9) in tRNA + S-adenosyl-L-methionine = H(+) + N(1)-
CC methylguanosine(9) in tRNA + S-adenosyl-L-homocysteine;
CC Xref=Rhea:RHEA:43156, Rhea:RHEA-COMP:10367, Rhea:RHEA-COMP:10368,
CC ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC ChEBI:CHEBI:73542, ChEBI:CHEBI:74269; EC=2.1.1.221;
CC Evidence={ECO:0000256|ARBA:ARBA00000855};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AHAT01001877; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_015200874.1; XM_015345388.1.
DR AlphaFoldDB; W5N4W0; -.
DR STRING; 7918.ENSLOCP00000015669; -.
DR Ensembl; ENSLOCT00000015699.1; ENSLOCP00000015669.1; ENSLOCG00000012735.1.
DR GeneID; 102692074; -.
DR KEGG; loc:102692074; -.
DR CTD; 158234; -.
DR eggNOG; KOG2967; Eukaryota.
DR GeneTree; ENSGT00530000063169; -.
DR HOGENOM; CLU_034384_8_0_1; -.
DR InParanoid; W5N4W0; -.
DR OMA; ALQAWFP; -.
DR OrthoDB; 2908056at2759; -.
DR Proteomes; UP000018468; Linkage group LG4.
DR Bgee; ENSLOCG00000012735; Expressed in ovary and 13 other cell types or tissues.
DR GO; GO:0005654; C:nucleoplasm; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0052905; F:tRNA (guanosine(9)-N1)-methyltransferase activity; IEA:UniProtKB-EC.
DR GO; GO:0000049; F:tRNA binding; IBA:GO_Central.
DR GO; GO:0002939; P:tRNA N1-guanine methylation; IBA:GO_Central.
DR CDD; cd18100; Trm10euk_B; 1.
DR Gene3D; 3.40.1280.30; -; 1.
DR InterPro; IPR028564; MT_TRM10-typ.
DR InterPro; IPR038459; MT_TRM10-typ_sf.
DR InterPro; IPR047911; Trm10_B_MTase_dom.
DR InterPro; IPR007356; tRNA_m1G_MeTrfase_euk.
DR InterPro; IPR016009; tRNA_MeTrfase_TRMD/TRM10.
DR PANTHER; PTHR13563; TRNA (GUANINE-9-) METHYLTRANSFERASE; 1.
DR PANTHER; PTHR13563:SF19; TRNA METHYLTRANSFERASE 10 HOMOLOG B; 1.
DR Pfam; PF01746; tRNA_m1G_MT; 1.
DR PROSITE; PS51675; SAM_MT_TRM10; 1.
PE 4: Predicted;
KW Methyltransferase {ECO:0000256|ARBA:ARBA00022603};
KW Reference proteome {ECO:0000313|Proteomes:UP000018468};
KW S-adenosyl-L-methionine {ECO:0000256|ARBA:ARBA00022691};
KW Transferase {ECO:0000256|ARBA:ARBA00022679}.
FT DOMAIN 124..321
FT /note="SAM-dependent MTase TRM10-type"
FT /evidence="ECO:0000259|PROSITE:PS51675"
FT REGION 1..39
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 85..116
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..16
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 341 AA; 38757 MW; FF14F390054D62CE CRC64;
MKGTMVERET EKSEHSLSCG MSKGQHKSED PHICGEESSS TQEMFELLRI DVDSETLKIP
RTDSQDSSCS RNVLRKQKNW EKTLAAKKGK RREEKQRRKL KRIEESGNML QSSQHSKRVV
KAITKERLIE AKTVGPRLCI DLSMTDLMSH KEISRLAGQI RRLYGSNRKA ANPFHLYLTG
LREDGLLYKE CLRKNDGFLN YVMDVTEDSW LDLFPLDSVI YLTPDSEHAL EHVDPDKVYI
LGGLVDESIQ KKITYQKAMD SCVCTARLPI QEYMVKKTNA KNFHSKILAI NQVFDILLTF
CETQSWPEAL KAGVPPGKGY VLYGESAPDA HLCAESALET L
//
