ID W5P2N4_SHEEP Unreviewed; 571 AA.
AC W5P2N4;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 54.
DE RecName: Full=Adenosylhomocysteinase {ECO:0000256|RuleBase:RU000548};
DE EC=3.13.2.1 {ECO:0000256|RuleBase:RU000548};
GN Name=AHCYL2 {ECO:0000313|Ensembl:ENSOARP00000004684.1};
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940 {ECO:0000313|Ensembl:ENSOARP00000004684.1, ECO:0000313|Proteomes:UP000002356};
RN [1] {ECO:0000313|Ensembl:ENSOARP00000004684.1, ECO:0000313|Proteomes:UP000002356}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Texel {ECO:0000313|Ensembl:ENSOARP00000004684.1,
RC ECO:0000313|Proteomes:UP000002356};
RX PubMed=20809919; DOI=10.1111/j.1365-2052.2010.02100.x;
RA Archibald A.L., Cockett N.E., Dalrymple B.P., Faraut T., Kijas J.W.,
RA Maddox J.F., McEwan J.C., Hutton Oddy V., Raadsma H.W., Wade C., Wang J.,
RA Wang W., Xun X.;
RT "The sheep genome reference sequence: a work in progress.";
RL Anim. Genet. 41:449-453(2010).
RN [2] {ECO:0000313|Ensembl:ENSOARP00000004684.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=H2O + S-adenosyl-L-homocysteine = adenosine + L-homocysteine;
CC Xref=Rhea:RHEA:21708, ChEBI:CHEBI:15377, ChEBI:CHEBI:16335,
CC ChEBI:CHEBI:57856, ChEBI:CHEBI:58199; EC=3.13.2.1;
CC Evidence={ECO:0000256|RuleBase:RU000548};
CC -!- COFACTOR:
CC Name=NAD(+); Xref=ChEBI:CHEBI:57540;
CC Evidence={ECO:0000256|RuleBase:RU000548};
CC Note=Binds 1 NAD(+) per subunit. {ECO:0000256|RuleBase:RU000548};
CC -!- PATHWAY: Amino-acid biosynthesis; L-homocysteine biosynthesis; L-
CC homocysteine from S-adenosyl-L-homocysteine: step 1/1.
CC {ECO:0000256|RuleBase:RU000548}.
CC -!- SIMILARITY: Belongs to the adenosylhomocysteinase family.
CC {ECO:0000256|ARBA:ARBA00007122, ECO:0000256|RuleBase:RU004166}.
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DR EMBL; AMGL01089006; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01089007; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01089008; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01089009; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01089010; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01089011; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01089012; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01089013; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01089014; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AMGL01089015; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR RefSeq; XP_014950865.1; XM_015095379.1.
DR AlphaFoldDB; W5P2N4; -.
DR SMR; W5P2N4; -.
DR STRING; 9940.ENSOARP00000004684; -.
DR PaxDb; 9940-ENSOARP00000004684; -.
DR Ensembl; ENSOART00000004765.1; ENSOARP00000004684.1; ENSOARG00000004375.1.
DR GeneID; 101114101; -.
DR CTD; 23382; -.
DR eggNOG; KOG1370; Eukaryota.
DR OMA; HIIWPDA; -.
DR OrthoDB; 120477at2759; -.
DR UniPathway; UPA00314; UER00076.
DR Proteomes; UP000002356; Chromosome 4.
DR Bgee; ENSOARG00000004375; Expressed in adult mammalian kidney and 55 other cell types or tissues.
DR ExpressionAtlas; W5P2N4; baseline.
DR GO; GO:0016787; F:hydrolase activity; IEA:UniProtKB-KW.
DR GO; GO:0006730; P:one-carbon metabolic process; IEA:UniProtKB-KW.
DR CDD; cd00401; SAHH; 1.
DR Gene3D; 3.40.50.1480; Adenosylhomocysteinase-like; 3.
DR Gene3D; 3.40.50.720; NAD(P)-binding Rossmann-like Domain; 1.
DR InterPro; IPR042172; Adenosylhomocyst_ase-like_sf.
DR InterPro; IPR000043; Adenosylhomocysteinase-like.
DR InterPro; IPR015878; Ado_hCys_hydrolase_NAD-bd.
DR InterPro; IPR036291; NAD(P)-bd_dom_sf.
DR InterPro; IPR020082; S-Ado-L-homoCys_hydrolase_CS.
DR NCBIfam; TIGR00936; ahcY; 1.
DR PANTHER; PTHR23420; ADENOSYLHOMOCYSTEINASE; 1.
DR PANTHER; PTHR23420:SF2; ADENOSYLHOMOCYSTEINASE 3; 1.
DR Pfam; PF05221; AdoHcyase; 1.
DR Pfam; PF00670; AdoHcyase_NAD; 1.
DR SMART; SM00996; AdoHcyase; 1.
DR SMART; SM00997; AdoHcyase_NAD; 1.
DR SUPFAM; SSF52283; Formate/glycerate dehydrogenase catalytic domain-like; 1.
DR SUPFAM; SSF51735; NAD(P)-binding Rossmann-fold domains; 1.
DR PROSITE; PS00738; ADOHCYASE_1; 1.
DR PROSITE; PS00739; ADOHCYASE_2; 1.
PE 3: Inferred from homology;
KW Endoplasmic reticulum {ECO:0000256|ARBA:ARBA00022824};
KW Hydrolase {ECO:0000256|RuleBase:RU000548};
KW NAD {ECO:0000256|ARBA:ARBA00023027, ECO:0000256|RuleBase:RU000548};
KW One-carbon metabolism {ECO:0000256|ARBA:ARBA00022563,
KW ECO:0000256|RuleBase:RU000548};
KW Reference proteome {ECO:0000313|Proteomes:UP000002356}.
FT DOMAIN 330..491
FT /note="S-adenosyl-L-homocysteine hydrolase NAD binding"
FT /evidence="ECO:0000259|SMART:SM00997"
FT REGION 1..144
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..28
FT /note="Pro residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 46..64
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 102..126
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ SEQUENCE 571 AA; 62826 MW; B7B458766EF6A46B CRC64;
MAPPAGGGDP EAPAPAAERP PAPGPGSGPA AALSPAAGKV PQASAMKRSD PHHQHQRHRD
GGEALVSPDG TVTEAPRTVK KQIQFADQKQ EFNKRPTKIG RRSLSRSISQ SSTDSYSSAA
SYTDSSDDET SPRDKQQKNS KGNSDFCVKN IKQAEFGRRE IEIAEQEMPA LMALRKRAQG
EKPLAGAKIV GCTHITAQTA VLMETLGALG AQCRWAACNI YSTLNEVAAA LAESGFPVFA
WKGESEDDFW WCIDRCVNVE GWQPNMILDD GGDLTHWIYK KYPNMFKKIK GIVEESVTGV
HRLYQLSKAG KLCVPAMNVN DSVTKQKFDN LYCCRESILD GLKRTTDMMF GGKQVVVCGY
GEVGKGCCAA LKAMGSIVYV TEIDPICALQ ACMDGFRLVK LNEVIRQVDI VITCTGNKNV
VTREHLDRMK NSCIVCNMGH SNTEIDVASL RTPELTWERV RSQVDHVIWP DGKRIVLLAE
GRLLNLSCST VPTFVLSITA TTQALALIEL YNAPEGRYKQ DVYLLPKKMD EYVASLHLPT
FDAHLTELTD EQAKYLGLNK NGPFKPNYYR Y
//
