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Database: UniProt
Entry: W5PUE5_SHEEP
LinkDB: W5PUE5_SHEEP
Original site: W5PUE5_SHEEP 
ID   W5PUE5_SHEEP            Unreviewed;      1010 AA.
AC   W5PUE5;
DT   16-APR-2014, integrated into UniProtKB/TrEMBL.
DT   16-APR-2014, sequence version 1.
DT   10-APR-2019, entry version 32.
DE   RecName: Full=Trifunctional purine biosynthetic protein adenosine-3 {ECO:0000256|RuleBase:RU363089};
DE   Includes:
DE     RecName: Full=Phosphoribosylamine--glycine ligase {ECO:0000256|RuleBase:RU363089};
DE              EC=6.3.4.13 {ECO:0000256|RuleBase:RU363089};
DE     AltName: Full=Glycinamide ribonucleotide synthetase {ECO:0000256|RuleBase:RU363089};
DE              Short=GARS {ECO:0000256|RuleBase:RU363089};
DE     AltName: Full=Phosphoribosylglycinamide synthetase {ECO:0000256|RuleBase:RU363089};
DE   Includes:
DE     RecName: Full=Phosphoribosylformylglycinamidine cyclo-ligase {ECO:0000256|RuleBase:RU363089};
DE              EC=6.3.3.1 {ECO:0000256|RuleBase:RU363089};
DE     AltName: Full=AIR synthase {ECO:0000256|RuleBase:RU363089};
DE              Short=AIRS {ECO:0000256|RuleBase:RU363089};
DE     AltName: Full=Phosphoribosyl-aminoimidazole synthetase {ECO:0000256|RuleBase:RU363089};
DE   Includes:
DE     RecName: Full=Phosphoribosylglycinamide formyltransferase {ECO:0000256|RuleBase:RU363089};
DE              EC=2.1.2.2 {ECO:0000256|RuleBase:RU363089};
DE     AltName: Full=5'-phosphoribosylglycinamide transformylase {ECO:0000256|RuleBase:RU363089};
DE     AltName: Full=GAR transformylase {ECO:0000256|RuleBase:RU363089};
DE              Short=GART {ECO:0000256|RuleBase:RU363089};
GN   Name=GART {ECO:0000313|Ensembl:ENSOARP00000014076};
OS   Ovis aries (Sheep).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Laurasiatheria; Cetartiodactyla; Ruminantia;
OC   Pecora; Bovidae; Caprinae; Ovis.
OX   NCBI_TaxID=9940 {ECO:0000313|Ensembl:ENSOARP00000014076, ECO:0000313|Proteomes:UP000002356};
RN   [1] {ECO:0000313|Ensembl:ENSOARP00000014076, ECO:0000313|Proteomes:UP000002356}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Texel {ECO:0000313|Ensembl:ENSOARP00000014076};
RX   PubMed=20809919; DOI=10.1111/j.1365-2052.2010.02100.x;
RA   Archibald A.L., Cockett N.E., Dalrymple B.P., Faraut T., Kijas J.W.,
RA   Maddox J.F., McEwan J.C., Hutton Oddy V., Raadsma H.W., Wade C.,
RA   Wang J., Wang W., Xun X.;
RT   "The sheep genome reference sequence: a work in progress.";
RL   Anim. Genet. 41:449-453(2010).
RN   [2] {ECO:0000313|Ensembl:ENSOARP00000014076}
RP   IDENTIFICATION.
RG   Ensembl;
RL   Submitted (FEB-2014) to UniProtKB.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=(6S)-10-formyltetrahydrofolate + N(1)-(5-phospho-D-
CC         ribosyl)glycinamide = (6S)-5,6,7,8-tetrahydrofolate + H(+) +
CC         N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide;
CC         Xref=Rhea:RHEA:15053, ChEBI:CHEBI:15378, ChEBI:CHEBI:57453,
CC         ChEBI:CHEBI:57454, ChEBI:CHEBI:58426, ChEBI:CHEBI:58457;
CC         EC=2.1.2.2; Evidence={ECO:0000256|RuleBase:RU363089};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + ATP
CC         = 5-amino-1-(5-phospho-beta-D-ribosyl)imidazole + ADP + H(+) +
CC         phosphate; Xref=Rhea:RHEA:23032, ChEBI:CHEBI:15378,
CC         ChEBI:CHEBI:30616, ChEBI:CHEBI:43474, ChEBI:CHEBI:58478,
CC         ChEBI:CHEBI:137981, ChEBI:CHEBI:456216; EC=6.3.3.1;
CC         Evidence={ECO:0000256|RuleBase:RU363089};
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-phospho-D-ribosylamine + ATP + glycine = ADP + H(+) +
CC         N(1)-(5-phospho-D-ribosyl)glycinamide + phosphate;
CC         Xref=Rhea:RHEA:17453, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:43474, ChEBI:CHEBI:57305, ChEBI:CHEBI:58089,
CC         ChEBI:CHEBI:58457, ChEBI:CHEBI:456216; EC=6.3.4.13;
CC         Evidence={ECO:0000256|RuleBase:RU363089};
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC       5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-
CC       phospho-D-ribosyl)glycinamide: step 2/2.
CC       {ECO:0000256|RuleBase:RU363089}.
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC       N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-
CC       ribose 1-diphosphate: step 2/2. {ECO:0000256|RuleBase:RU363089}.
CC   -!- PATHWAY: Purine metabolism; IMP biosynthesis via de novo pathway;
CC       N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-
CC       phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
CC       {ECO:0000256|RuleBase:RU363089}.
CC   -!- SIMILARITY: In the C-terminal section; belongs to the GART family.
CC       {ECO:0000256|RuleBase:RU363089}.
CC   -!- SIMILARITY: In the N-terminal section; belongs to the GARS family.
CC       {ECO:0000256|RuleBase:RU363089}.
CC   -!- SIMILARITY: In the central section; belongs to the AIR synthase
CC       family. {ECO:0000256|RuleBase:RU363089}.
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DR   EMBL; AMGL01005687; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   RefSeq; XP_004002809.1; XM_004002760.2.
DR   RefSeq; XP_012041570.1; XM_012186180.2.
DR   RefSeq; XP_012041576.1; XM_012186186.1.
DR   RefSeq; XP_012041580.1; XM_012186190.1.
DR   RefSeq; XP_012041584.1; XM_012186194.2.
DR   RefSeq; XP_012041590.1; XM_012186200.2.
DR   RefSeq; XP_014947848.1; XM_015092362.1.
DR   RefSeq; XP_014947850.1; XM_015092364.1.
DR   STRING; 9940.ENSOARP00000014076; -.
DR   Ensembl; ENSOART00000014283; ENSOARP00000014076; ENSOARG00000013139.
DR   GeneID; 101121731; -.
DR   KEGG; oas:101121731; -.
DR   CTD; 2618; -.
DR   GeneTree; ENSGT00390000000292; -.
DR   KO; K11787; -.
DR   OMA; LLERHNC; -.
DR   OrthoDB; 105366at2759; -.
DR   UniPathway; UPA00074; UER00125.
DR   Proteomes; UP000002356; Chromosome 1.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0004637; F:phosphoribosylamine-glycine ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0004641; F:phosphoribosylformylglycinamidine cyclo-ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0004644; F:phosphoribosylglycinamide formyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006189; P:'de novo' IMP biosynthetic process; IEA:UniProtKB-UniRule.
DR   GO; GO:0009113; P:purine nucleobase biosynthetic process; IEA:UniProtKB-UniRule.
DR   CDD; cd08645; FMT_core_GART; 1.
DR   CDD; cd02196; PurM; 1.
DR   Gene3D; 3.30.1330.10; -; 1.
DR   Gene3D; 3.30.1490.20; -; 1.
DR   Gene3D; 3.90.600.10; -; 1.
DR   Gene3D; 3.90.650.10; -; 1.
DR   HAMAP; MF_00741; AIRS; 1.
DR   HAMAP; MF_00138; GARS; 1.
DR   HAMAP; MF_01930; PurN; 1.
DR   InterPro; IPR011761; ATP-grasp.
DR   InterPro; IPR013815; ATP_grasp_subdomain_1.
DR   InterPro; IPR002376; Formyl_transf_N.
DR   InterPro; IPR036477; Formyl_transf_N_sf.
DR   InterPro; IPR004607; GART.
DR   InterPro; IPR001555; GART_AS.
DR   InterPro; IPR016185; PreATP-grasp_dom_sf.
DR   InterPro; IPR020561; PRibGlycinamid_synth_ATP-grasp.
DR   InterPro; IPR000115; PRibGlycinamide_synth.
DR   InterPro; IPR020560; PRibGlycinamide_synth_C-dom.
DR   InterPro; IPR037123; PRibGlycinamide_synth_C_sf.
DR   InterPro; IPR020559; PRibGlycinamide_synth_CS.
DR   InterPro; IPR020562; PRibGlycinamide_synth_N.
DR   InterPro; IPR010918; PurM-like_C_dom.
DR   InterPro; IPR036676; PurM-like_C_sf.
DR   InterPro; IPR016188; PurM-like_N.
DR   InterPro; IPR036921; PurM-like_N_sf.
DR   InterPro; IPR004733; PurM_cligase.
DR   InterPro; IPR011054; Rudment_hybrid_motif.
DR   PANTHER; PTHR10520; PTHR10520; 1.
DR   Pfam; PF00586; AIRS; 1.
DR   Pfam; PF02769; AIRS_C; 1.
DR   Pfam; PF00551; Formyl_trans_N; 1.
DR   Pfam; PF01071; GARS_A; 1.
DR   Pfam; PF02843; GARS_C; 1.
DR   Pfam; PF02844; GARS_N; 1.
DR   SMART; SM01210; GARS_C; 1.
DR   SUPFAM; SSF51246; SSF51246; 1.
DR   SUPFAM; SSF52440; SSF52440; 1.
DR   SUPFAM; SSF53328; SSF53328; 1.
DR   SUPFAM; SSF55326; SSF55326; 1.
DR   SUPFAM; SSF56042; SSF56042; 1.
DR   TIGRFAMs; TIGR00877; purD; 1.
DR   TIGRFAMs; TIGR00878; purM; 1.
DR   TIGRFAMs; TIGR00639; PurN; 1.
DR   PROSITE; PS50975; ATP_GRASP; 1.
DR   PROSITE; PS00184; GARS; 1.
DR   PROSITE; PS00373; GART; 1.
PE   3: Inferred from homology;
KW   ATP-binding {ECO:0000256|PROSITE-ProRule:PRU00409,
KW   ECO:0000256|RuleBase:RU363089};
KW   Complete proteome {ECO:0000313|Proteomes:UP000002356};
KW   Ligase {ECO:0000256|RuleBase:RU363089};
KW   Manganese {ECO:0000256|RuleBase:RU363089};
KW   Metal-binding {ECO:0000256|RuleBase:RU363089};
KW   Multifunctional enzyme {ECO:0000256|RuleBase:RU363089};
KW   Nucleotide-binding {ECO:0000256|PROSITE-ProRule:PRU00409,
KW   ECO:0000256|RuleBase:RU363089};
KW   Purine biosynthesis {ECO:0000256|RuleBase:RU363089};
KW   Reference proteome {ECO:0000313|Proteomes:UP000002356}.
FT   DOMAIN      111    318       ATP-grasp. {ECO:0000259|PROSITE:PS50975}.
SQ   SEQUENCE   1010 AA;  107858 MW;  583329AC69D68780 CRC64;
     MAARVLVIGN GGREHTLAWK LAQSTHVKQV LVTPGNAGTA CSEKISNTDI SISDHTALAQ
     FCKDEKIEFV VVGPEAPLAA GIVGNLNSVG VRCFGPTAEA AQLESSKRFA KEFMDRHGIS
     TARWKAFTKP EKACDFIMSA DFPALVVKAS GLAAGKGVII AKSKEEACDA VREIMQGKAF
     GEAGETVVIE ELLEGEEVSC LCFTDGRTVA PMPPAQDHKR LLEGDQGPNT GGMGAYCPAP
     QVSKDLLLKI KNTILQRTVD GMQEEGMPYT GVLYAGIMLT KNGPKVLEFN CRFGDPECQV
     ILPLLKSDLY EVIQSTLDGL LCTSLPVWLD NCAAVTVVMA SKGYPGDYTK GVEITGFPEA
     RALGLEVFQA GTALKDGKVV TNGGRILSVT AIRENLISAL EQARKGLAAI KFEGAVYRKD
     IGFRAIAFLQ QTRGLTYKES GVDIAAGNML VQKIKPLAKA TSRPGCDVDL GGFAGLFDLK
     AAGFTDPLLA CGTDGVGTKL KIAQQCGKHE TIGQDLVAMC VNDILAQGAE PLFFLDYFSC
     GKLDLHTTEA VIAGIAQACR KAGCALLGGE TAEMPDMYPP GEYDLAGFAV GAMERDQKLP
     HLERITEGDA VIGIASSGLH SNGFSLVRKI VANSSLQYSS PAPVGCGDQT LGDLLLTPTR
     IYSHSLLPVL RSGRVKAVAH ITGGGLLENI PRVLPQKLGV NLDAQTWRVP RIFSWLQQEG
     HLSEEEMART FNCGIGAALV VSEDLVKQTL QDIEQHQQEA WVIGRVVACP EGSPRVKVEH
     LIETMQINGS VLENGTLRNH ISVQPKKARV AVLISGTGSN LQALIDSTRE PGSLAHIVVV
     ISNKAAVAGL DKAEKAGIPT RVINHKLYKN RAAFDNAVDE VLEEFSTDIV CLAGFMRILS
     GPFVRKWNGK MLNIHPSLLP SFKGSNAHEQ VLDAGVTITG CTVHFVAEDV DAGQIILQEA
     VPVKRGDTVE TLSERVKLAE HKIFPSALQL VASGAVCLGE NGRICWVTED
//
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