ID W5PUE6_SHEEP Unreviewed; 1251 AA.
AC W5PUE6;
DT 16-APR-2014, integrated into UniProtKB/TrEMBL.
DT 16-APR-2014, sequence version 1.
DT 27-MAR-2024, entry version 55.
DE RecName: Full=Apoptotic protease-activating factor 1 {ECO:0000256|PIRNR:PIRNR037646};
DE Short=APAF-1 {ECO:0000256|PIRNR:PIRNR037646};
GN Name=APAF1 {ECO:0000313|Ensembl:ENSOARP00000014077.1};
OS Ovis aries (Sheep).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC Caprinae; Ovis.
OX NCBI_TaxID=9940 {ECO:0000313|Ensembl:ENSOARP00000014077.1, ECO:0000313|Proteomes:UP000002356};
RN [1] {ECO:0000313|Ensembl:ENSOARP00000014077.1, ECO:0000313|Proteomes:UP000002356}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Texel {ECO:0000313|Ensembl:ENSOARP00000014077.1,
RC ECO:0000313|Proteomes:UP000002356};
RX PubMed=20809919; DOI=10.1111/j.1365-2052.2010.02100.x;
RA Archibald A.L., Cockett N.E., Dalrymple B.P., Faraut T., Kijas J.W.,
RA Maddox J.F., McEwan J.C., Hutton Oddy V., Raadsma H.W., Wade C., Wang J.,
RA Wang W., Xun X.;
RT "The sheep genome reference sequence: a work in progress.";
RL Anim. Genet. 41:449-453(2010).
RN [2] {ECO:0000313|Ensembl:ENSOARP00000014077.1}
RP IDENTIFICATION.
RG Ensembl;
RL Submitted (NOV-2023) to UniProtKB.
CC -!- FUNCTION: Oligomeric Apaf-1 mediates the cytochrome c-dependent
CC autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the
CC activation of caspase-3 and apoptosis. This activation requires ATP.
CC {ECO:0000256|PIRNR:PIRNR037646}.
CC -!- SUBUNIT: Monomer. Oligomerizes upon binding of cytochrome c and dATP.
CC {ECO:0000256|PIRNR:PIRNR037646}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000256|PIRNR:PIRNR037646}.
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DR EMBL; AMGL01081824; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR AlphaFoldDB; W5PUE6; -.
DR SMR; W5PUE6; -.
DR STRING; 9940.ENSOARP00000014077; -.
DR PaxDb; 9940-ENSOARP00000014077; -.
DR Ensembl; ENSOART00000014284.1; ENSOARP00000014077.1; ENSOARG00000013137.1.
DR eggNOG; KOG4155; Eukaryota.
DR eggNOG; KOG4658; Eukaryota.
DR HOGENOM; CLU_005071_1_0_1; -.
DR OMA; YHMANAN; -.
DR Proteomes; UP000002356; Chromosome 3.
DR Bgee; ENSOARG00000013137; Expressed in thymus and 55 other cell types or tissues.
DR GO; GO:0043293; C:apoptosome; IEA:InterPro.
DR GO; GO:0043531; F:ADP binding; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0097190; P:apoptotic signaling pathway; IEA:InterPro.
DR GO; GO:0042981; P:regulation of apoptotic process; IEA:InterPro.
DR CDD; cd08323; CARD_APAF1; 1.
DR CDD; cd00200; WD40; 2.
DR Gene3D; 1.25.40.370; -; 1.
DR Gene3D; 1.10.533.10; Death Domain, Fas; 1.
DR Gene3D; 1.10.8.430; Helical domain of apoptotic protease-activating factors; 1.
DR Gene3D; 3.40.50.300; P-loop containing nucleotide triphosphate hydrolases; 1.
DR Gene3D; 1.10.10.10; Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain; 1.
DR Gene3D; 2.130.10.10; YVTN repeat-like/Quinoprotein amine dehydrogenase; 2.
DR InterPro; IPR017251; Apaf-1.
DR InterPro; IPR041452; APAF1_C.
DR InterPro; IPR037963; APAF1_CARD_dom.
DR InterPro; IPR048975; APAF1_WHD.
DR InterPro; IPR042197; Apaf_helical.
DR InterPro; IPR024977; Apc4-like_WD40_dom.
DR InterPro; IPR001315; CARD.
DR InterPro; IPR011029; DEATH-like_dom_sf.
DR InterPro; IPR020472; G-protein_beta_WD-40_rep.
DR InterPro; IPR002182; NB-ARC.
DR InterPro; IPR027417; P-loop_NTPase.
DR InterPro; IPR015943; WD40/YVTN_repeat-like_dom_sf.
DR InterPro; IPR019775; WD40_repeat_CS.
DR InterPro; IPR036322; WD40_repeat_dom_sf.
DR InterPro; IPR001680; WD40_rpt.
DR InterPro; IPR036388; WH-like_DNA-bd_sf.
DR PANTHER; PTHR22845; APOPTOTIC PROTEASE-ACTIVATING FACTOR 1; 1.
DR PANTHER; PTHR22845:SF5; APOPTOTIC PROTEASE-ACTIVATING FACTOR 1; 1.
DR Pfam; PF12894; ANAPC4_WD40; 1.
DR Pfam; PF21296; APAF-1-like_WHD; 1.
DR Pfam; PF17908; APAF1_C; 1.
DR Pfam; PF00619; CARD; 1.
DR Pfam; PF00931; NB-ARC; 1.
DR Pfam; PF00400; WD40; 8.
DR PIRSF; PIRSF037646; Apop_pept_activating-1; 1.
DR PRINTS; PR00364; DISEASERSIST.
DR PRINTS; PR00320; GPROTEINBRPT.
DR SMART; SM00320; WD40; 13.
DR SUPFAM; SSF47986; DEATH domain; 1.
DR SUPFAM; SSF52540; P-loop containing nucleoside triphosphate hydrolases; 1.
DR SUPFAM; SSF50978; WD40 repeat-like; 2.
DR PROSITE; PS50209; CARD; 1.
DR PROSITE; PS00678; WD_REPEATS_1; 3.
DR PROSITE; PS50082; WD_REPEATS_2; 9.
DR PROSITE; PS50294; WD_REPEATS_REGION; 6.
PE 4: Predicted;
KW Apoptosis {ECO:0000256|ARBA:ARBA00022703, ECO:0000256|PIRNR:PIRNR037646};
KW ATP-binding {ECO:0000256|PIRNR:PIRNR037646, ECO:0000256|PIRSR:PIRSR037646-
KW 50}; Cytoplasm {ECO:0000256|PIRNR:PIRNR037646};
KW Nucleotide-binding {ECO:0000256|PIRNR:PIRNR037646,
KW ECO:0000256|PIRSR:PIRSR037646-50};
KW Reference proteome {ECO:0000313|Proteomes:UP000002356};
KW Repeat {ECO:0000256|ARBA:ARBA00022737};
KW WD repeat {ECO:0000256|ARBA:ARBA00022574, ECO:0000256|PROSITE-
KW ProRule:PRU00221}.
FT DOMAIN 3..92
FT /note="CARD"
FT /evidence="ECO:0000259|PROSITE:PS50209"
FT REPEAT 613..654
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 655..696
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 697..740
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 741..782
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 880..912
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 1001..1033
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 1042..1082
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 1083..1117
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT REPEAT 1125..1166
FT /note="WD"
FT /evidence="ECO:0000256|PROSITE-ProRule:PRU00221"
FT BINDING 156..163
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PIRSR:PIRSR037646-50"
FT BINDING 267
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000256|PIRSR:PIRSR037646-50"
SQ SEQUENCE 1251 AA; 142092 MW; 21CEB741DD504AA7 CRC64;
GKMDAKARNC LLQHREALER DIKTSYIMDH MISNGVLTVS EEEKVKNEPT QRQRAAMLIK
MILKKDNHSY ISFYNALLHE GYKDLAALLH GGIPVVSSSD GKDSVSGITS YVRTVLCEGG
VPQRPVVFVT RKKLVNAIQQ KLSKLNGEPG WVTIYGMAGC GKSVLAAEAV RDHSFLEDCF
PGGVHWVSVG KQDKSGLLMK LQNLCARLDQ DESFSQRLPL NIEEAKDRLR VLMLRKHPRS
LLILDDIWDP WVLKAFDNQC QILLTTRDKS VTDSVMGPKY VVPVESGLGK EKALEILSLF
VNMKKADLPE QAHSIIQECK GSPLVVSLIG ALLRDFPNRW EYYLRQLQNK QFKRIRKSSS
YDYEALDEAM SISVEMLRED IKDYYTDLSI IQKDVKVPTK VLCILWDLEM EEVEDILQEF
VNKSLLFCDR NGKSFLYYLH DLQVDFLTEK NRSQLQDLHK KMVTQFQRHH QLHSLTPDQE
DCMYWYNFLA YHMASANMHK ELCALMFSLD WIKAKTELVG PAHLIHEFVE YRHILDEKDG
TDCENFQEFL SLNGHLLGRQ PFPNIVQLGL CELETSEVYR QAKLQAKQEV DNGMLYLEWV
NKKNMKNLSR LVVRPHTDAV YHACFSEDGQ RIASCGADKT LQVFKAETGE KLLEIKAHED
EVLCCAFSAD DRFIATCSVD KKVKIWNSMT GELVRIYDEH SEQVNCCHFT NNSSHLLLAT
GSNDYFLKLW DLNQEECRNT MFGHTNSVSH CRFSPDDQVL ASCSADGTLK LWDVKSANER
KSINVKQFFL NSEEPQEDLE VIVKCCSWSA DGAKIMVAAK NKVFLFDIHT SGLLAEIHMG
HHSTVQYCDF SPHNHLAVVA LSQYCVELWS MDSGLKVADC RGHLSWVHCV MFSPDGSSFL
TSSDDQTVRL WETKKVCKNS ATVLKQEIDV VFQENEVTVL AVDNIRRLQL INGNTGQIDY
LTEAQVSCCC LSPHLQYLAF GAEDGAIQIL ELLNNRIFQS RNAHKTTVRH IQFTADGKTL
ISSSDDSAVQ IWNWQSEDPF FLQAHQETVK DFKLLKNSRL LSWSFDGTVK VWNITTRRIE
KDFVCHQDTV LSCDISSDGA KFSSTSADKT AKIWSFELLS PLHELRGHKG CVRCSSFSVD
NTMLATGDDN GEIRIWNVSN GELLHLCAPI SVEEGASTHG GWVTDLCFSP DTKMLVSAGG
YLKWWNVLTG ESSQTFYTNG TNLKKIHVSP DFKTYVTVDN LGILYILKIL E
//
